Biochemical effect of some feed additives and immunological response in broilder chickens

This study was investigated the effects of dietary supplementation with prebiotics beta-glucan, MOS 500 and Bio MOS [registered sign] on the broilers performance, biochemical parameters and immune response for 5 weeks. One hundred and twenty 1-day-old broiler chickens were randomly assigned to 4 dietary treatment groups as follows: control, beta-glucan [1g/kg], MOS 500 [0.5g/kg] and Bio MOS [registered sign] [1g/kg]. Each treatment was divided into three replicates [ten birds/ replicate]. The overall BW gains of birds treated with prebiotics were significantly [P <0.01] higher than those of the control group. During the experiment, the birds fed supplemental Bio MOS [registered sign] gave the best FCR followed by beta-glucan MOS 500 and then the control group. Addition of these prebiotics were improved the levels of serum total protein and globulin which in turn support the immune function against NDV and IBDV. In addition to, supplementation of broiler chicks by beta-glucan, MOS 500 and Bio-MOS [registered sign] had no effect on the liver transmination enzymes and some kidney function which represent the non pathological metabolism of the liver and kidneys indicating their safety supplementation in broiler feed

Effect of dietary enzymes supplementation on growth performance, immune response, some blood parameters and carcass quality of broiler chicks

One hundred and twenty, day old age healthy cobb broiler chicks were used to study the influence of dietary supplementation of enzymes on growth performance, immune response, some blood parameters and carcass traits of broiler chickens. Broiler chicks were allotted into four groups; chicks of group 1 [the control] were fed on basal diet only, chicks of group 2 were fed on the basal diet supplemented by Deltazyme plus [registered sign] at 0.5 g/kg diet, chicks of group 3 received 95%basal diet +5% wheat bran and chicks of group 4 received the same diet of group 3 supplemented with Deltazyme plus [registered sign] supplementation improved significantly [P 0.05] increased in chicks of group 4 when compared with those of group 3. Dietary enzymes supplementation [groups 2 and 4] reduced daily feed intake when compared with those groups 1 and 3 respectively. Dietary supplementation of Deltazyme plus [registered sign] at 0.5 g/kg diet improved significantly [P

Detection of reticuloendotheliosis virus as contaminant of fowl pox vaccines

This study was designated to detect reticuloendotheliosis virus [REV] as a contaminant in fowl pox vaccines. A total of 30 fowl pox vaccine samples were examined for the presence of REV using the in vitro and in vivo methods. In the in vitro test, the fowl pox vaccine samples were inoculated into chicken embryo fibroblast [CEF] cultures prepared from SPF embryonated chicken eggs and examined by PCR test for detection of REV. In the in vivo test, each fowl pox vaccine sample was inoculated in five days old SPF chicks and kept under observation up to 12 weeks post inoculation [P1]; serum samples were collected at 15th, 30th, and 45th days P1 for the detection of antibodies against REV by commercial ELISA kit, and tissue samples were collected at 8th and 12th weeks PI for histopathological examination. Results revealed that: only one imported vaccine sample gave positive results by PCR test a product of 291- bp was obtained by the vaccine sample. Serum samples collected from positive vaccine batch were tested for REV antibodies by ELISA test and the sera were positive. Histopathological examination for liver, spleen and bursa of fabricius revealed the presence of tumor cells in the examined organs and these changes confirmed the results obtained by PCR and ELISA tests and indicated that the sample is contaminated with REV. The data clearly indicated that screening of all commercial poultry vaccines to be virus free is an important factor in assuring the biosafety of animal vaccines

Eimeria tenella infection during aflatoxicosis and administration of anticoccidial drugs

To investigate the effect[s] of aflatoxicosis on E. tenella infection, one day-old, male LSL Brown chicks were randomly allotted into 10 equal treatments. Each treatment had 3 replicates with 10 birds/replicate. Aflatoxicosis was induced by contaminating the starter ration with 5 ppm of aflatoxin during the first two weeks of age, followed by 2.5 ppm for the third week. Coccidia infection was done by intracrop administration of 20.000 living sporulated E.tenella oocysts/bird at 14 days of age. Lasaloci and clopidol were prophylactically used as anticoccidial agents at doses of 75 and 125 ppm, respectively, from one day old to the 35th day of age. The main findings were as follows: Aflatoxin treatment of non-infected birds significantly reduced body weignts compared to non-toxicosed and infected or non-infected birds. Combined aflatoxicosis and caecal coccidiosdis caused more depression of body weight and more mortality than either alone. E. tenella infection resulted in 23.33% mortality and clopidol medicated birth showed 6.67% mortality. However, lasalocid prevented completely mortality due to E. tenella infection. Aflatoxin alone in ration caused 20% mortality, while aflatoxin with E. tenella caused 36.67% mortality. Lasalocid and clopidol-medicated birds treated with aflatoxin had 10% and 16.67% mortalities respectively. Lasalocid treated surpassed clopidol-treated birds in the reductiojn of oocyst production and lesion scores. Aflatoxin did not interfere with the life cycle of E. tenella as judged by the daily output of oocysts

contribution towards the virulence of candida species for chicken embryos

In this work C. albicans, C. tropicalis, C. krusei, C. parapsillosis and C. guilliermondi, were isolated and identified. Chicken embryos were used for studies of the virulence of C and ida species by inoculation in the yolk sac or on the chorioallantoic membrane. The above-mentioned C and ida species showed higher percentage of embryonic death and positive reteroculturing. The source of isolates had no effect on death rate whether yolk sac or chorioallantoic membrane routes were used, while the effect of routes on death rate was significant at [5% level] as shown from the statistical analysis of the obtained data

Incidence of salmonellae in poultry feeds and by-products

A total of 192 broiler ration samples collected from feeders and feed storage rooms yielded the following serotypes: S. gallinarum- pullorum [4], S. typhimurium [1] and S. norwish [1]. Furthermore, six isolates of S. typhimurium and one isolate of S. newport were recovered from 87 broiler concentrates, fish meal and blood meal