RESUMO
@#Immunotherapy cytokine preparations have important clinical value in anti-infection,anti-tumor,anti-rejection,autoimmune diseases and tissue repair,while their clinical application is limited due to the adverse reactions caused by short half-life,non-specific and adverse biodistribution of early cytokine preparations. With the in-depth understanding of the principle of cytokine-receptor interaction and cytokine-mediated signaling pathways,cytokine engineering for modification has become an effective cytokine design route. This paper mainly summarized the engineering design technology of cytokines,such as the design,targeting and delivery of recombinant cytokines,mRNA expression and cell technology,as well as the possible development directions of cytokine engineering in the future and the challenges in the process of clinical transformation,aiming to provide relevant technical references for the research and development of cytokine genetic engineering technology.