Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Adicionar filtros








Intervalo de ano
1.
The Korean Journal of Laboratory Medicine ; : 138-146, 2010.
Artigo em Coreano | WPRIM | ID: wpr-151628

RESUMO

BACKGROUND: We developed and evaluated the utility of a multiplex real-time PCR assay that uses melting curve analysis and allows simultaneous identification of vancomycin-resistant genotypes and clinically relevant enterococci. METHODS: The specificity of the assay was tested using 4 reference strains of vancomycin-resistant enterococci (VRE) and 2 reference strains of vancomycin-susceptible enterococci. Ninety-three clinical isolates of enterococci with different glycopeptide-resistant phenotypes were genotyped and identified using a multiplex real-time PCR assay and melting curve analysis. RESULTS: Representative melting curves were obtained for Enterococcus faecium, Enterococcus faecalis, vanA-containing E. faecium, vanB-containing E. faecalis, Enterococcus gallinarum, and Enterococcus casseliflavus. Phenotypic and genotypic analysis of the isolates revealed same results for 82 enterococcal isolates, while in 4 isolates, the glycopeptide-resistant phenotypes were inconsistent with the glycopeptide-resistant genotypes and in the 4 other isolates, species could not be accurately identified. Three isolates with mixed strains, which were detected by the PCR assay, could not be correctly identified using phenotypic methods. CONCLUSIONS: VRE genotyping and identification of clinically relevant enterococci were rapidly and correctly performed using multiplex real-time PCR assay and melting curve analysis.


Assuntos
Proteínas de Bactérias/genética , Carbono-Oxigênio Ligases/genética , DNA Bacteriano/genética , Enterococcus/genética , Enterococcus faecalis/genética , Enterococcus faecium/genética , Genótipo , Desnaturação de Ácido Nucleico , Peptídeo Sintases/genética , Fenótipo , Reação em Cadeia da Polimerase , Resistência a Vancomicina/genética
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA