Analysis on Polymorphism of Platelet Antigen Gene in Shandong Han Population / 中国实验血液学杂志

OBJECTIVE@#To study the Polymorphism of the human platelet antigen(HPA) gene 1-17 and human leukocyte antigen(HLA) gene-A and B locus in Shandong Han population.@*METHODS@#A total of 962 samples from routine voluntary platelet donors were genotyped for HPA1-17 system and HLA-A site, B by PCR-SSP and PCR-SSOP respectively．Gene frequencies were calculated by counting. HPA1-17 and HLA genotype combinations were analyzed by Arelequin 3.5.@*RESULTS@#The gene frequencies of HPA-la, -1b, HPA-2a, -2b, HPA-3a, -3b, HPA-4a, -4b, HPA-5a, -5b, HPA-6a, -6b, HPA-15a, -15b were 0.9918, 0.0082, 0.9419, 0.0592, 0.5841, 0.4174, 0.9969, 0.0031, 0.9892, 0.0108, 0.9835, 0.0175,0.5488 and 0.4512, respectively． The most common HPA genotype combination was HPA-(1, 2, 4, 5, 6, 7-14, 16, 17) aa-3ab-15ab (0.2048). Moreover, HLA-A*2(0.3094) and HLA-B*13(0.1513) showed the highest frequency in their respective locus. The most common HLA genotype combination was HLA-A*2-B*13(0.1397) .@*CONCLUSION@#Distributions of HPA and HLA show high polymorphism in Shandong Han population. The ethnic and territorial difference of HPA distribution is also confirmed. It is imperative to establish local genetic database of volunteer platelet donors.

Prevalence of thyroid nodules and influencing factors among employees of a company in Qingdao / 中华预防医学杂志

<p><b>OBJECTIVE</b>To investigate the prevalence of thyroid nodules and influencing factors among employees of a company in Qingdao.</p><p><b>METHODS</b>Through questionnaires, physical examination, thyroid ultrasonography and urinary iodine test, personal health information and examinations of thyroid nodules of 9612 serving and retired employees in a company in 2010 who had lived in Qingdao for more than 5 years were collected to investigate the prevalence of thyroid nodules and its influencing factors.</p><p><b>RESULTS</b>The prevalence of thyroid nodules was 36.56% (3514/9612). The prevalence rates of single nodules and multiple nodules were 18.39% (1768/9612) and 18.16% (1746/9612) respectively. With increasing urinary iodine level, the prevalence of multiple thyroid nodules decreased from 25.51% (366/14350)(urinary iodine < or = 100 microg/L) to 12.99% (214/1648) (urinary iodine > 300 microg/L) showing a clear downward trend (chi2 = 67.11, P < 0.01). The prevalence rates of thyroid nodules among males and females were 31.28% (2283/7299) and 53.22% (1231/2313) (P < 0.01) respectively. With increasing age, the prevalence of thyroid nodules gradually increased from 7.95% (67/843) (19 - 29 years old) to 58.81% (267/454) ( >70 years old) among males and increased from 23.74% (33/139) (19 - 29 years old) to 80.38% (127/158) ( >70 years old) among females. The prevalence of thyroid nodules among males and females showed an increasing trend with age (male: chi2 = 434.12, P < 0.01; female: chi2 = 40.74, P < 0.01). The results of logistic regression showed that compared with males, females were more susceptible to thyroid nodules (OR = 2.809, 95% CI: 2.444 - 3.228). Smoking (OR = 1.394, 95% CI: 1.249 - 1.556), hypertension (OR = 1.155, 95% CI: 1.040 - 1.282), diabetes ( OR = 1.228, 95% CI: 1.039 - 1.452) and overweight (OR = 1.199, 95% CI: 1.078 - 1.333) might be risk factors for thyroid nodules after adjusting for age and gender.</p><p><b>CONCLUSION</b>The prevalence of thyroid nodules was high among the employees. In order to reduce the thyroid nodules, smoking cessation and weight control should be promoted. Blood pressure and glucose level should be actively controlled for patients with hypertension and (or) diabetes.</p>

Comparison of radiography and magnetic resonance imaging in detecting arthropathies in patients with hemophilia / 中国医学科学院学报

<p><b>OBJECTIVE</b>To compare magnetic resonance imaging (MRI) and radiography in detecting arthropathies in patients with hemophilia.</p><p><b>METHODS</b>Of 41 symptomatic joint images in the 14 patients with hemophilia, each joint was examined with both radiography and MRI within the same day. Imaging findings with both two modes were compared.</p><p><b>RESULTS</b>Soft tissue swelling or joint effusion was observed in 33 joints by radiographs and in 34 joints by MRI. Joint erosions were demonstrated in 34 joints by MRI and in 20 joints by radiographs. Joint cysts were shown in 21 joints by MRI and in 9 joints by radiographs. Significant differences in the detection of erosion and cyst were found between radiography and MRI (P < 0. 05). MRI showed improvement for detecting more foci of both erosion and cyst than radiography. Bone marrow edema in 14 joints, hemorrhage in 34 joints, and synovial hypertrophy in 27 joints were revealed only by MRI.</p><p><b>CONCLUSION</b>MRI is superior to conventional radiography in detecting the abnormal changes, and should be considered as the imaging mode of choice in evaluating hemophilic arthropathies.</p>

Application of multi-slice spiral CT in the diagnosis of pulmonary hypertension / 中国医学科学院学报

<p><b>OBJECTIVE</b>To evaluate the utility of multi-slice spiral CT in the diagnosis of pulmonary hypertension.</p><p><b>METHODS</b>The clinical and multi-slice spiral CT data of 22 patients with pulmonary hypertension were retrospectively analyzed. The diagnoses included chronic thromboembolic pulmonary hypertension (CTEPH) (n = 9), primary pulmonary hypertension (PPH) (n = 7), connective tissue diseases (n = 2), portal-pulmonary hypertension (n = 2), eosinophilia-myalgia syndrome (n = 1), and fibrosing mediastinitis (n = 1). Lung parenchyma, such as ground-glass opacity, mosaic attenuation and septal thickness, mediastinum, pulmonary vascular, right ventricular enlargement and interventricular septum displacement were evaluated on CT.</p><p><b>RESULTS</b>CT pulmonary angiography (CTPA) accurately diagnosed 95% of pulmonary hypertension and the signs of right ventricular dysfunction. Pulmonary embolism was found in 9 patients. The incidence of mosaic attenuation was significantly higher in CTECH than in PPH (P = 0.034).</p><p><b>CONCLUSION</b>CTPA can accurately diagnose pulmonary hypertension and thereby provide useful information for the etiological diagnosis.</p>

Techniques and clinical applications of 16 multislice helical CT in colonic diseases / 中国医学科学院学报

<p><b>OBJECTIVE</b>To evaluate the techniques and clinical applications of 16 multislice helical CT in colonic lesions.</p><p><b>METHODS</b>Eighty-one patients including 54 colorectal carcinomas, 5 adenomas, 1 non-Hodgkin's lymphoma, 6 inflammatory bowel diseases, and other 15 cases underwent volume scanning using 16 multislice helical CT. Four types of reconstruction included multiple planar reconstruction, shaded surface display, raysum, and CT virtual colonoscopy.</p><p><b>RESULTS</b>Complete colon could be shown in all patients. The lesions' morphology, number, size, location, intestinal cavity, pericolonic changes, and other abdominal organs were satisfactorily shown by CT.</p><p><b>CONCLUSIONS</b>Sixteen multislice helical CT colonography is a valuable imaging technique for detecting colonic diseases. It is effective in diagnosis and treatment planning. It can display the portions of colon that is inaccessible at colonoscopy.</p>

Inhibitors of Na+/H+ and Na+/Ca2+ exchange depress intracellular calcium elevation induced by ischemia/reperfusion in rat cardiac myocytes / 生理学报

An increase in cytosolic free calcium has been shown to occur during ischemia in perfused hearts and plays a pivotal role in ischemia/reperfusion injury. The objective of this study was to investigate the contributions of Na(+)/H(+) exchange and Na(+)/Ca(2+) exchange to changes in intracellular calcium ([Ca(2+)](i)) during simulated ischemia and reperfusion in quiescent isolated rat cardiac myocytes. [Ca(2+)](i) was measured by laser confocal microscope using the fluorescent indicator Fluo 3 and expressed as the corrected intensity of Fluo 3 fluorescence. [Ca(2+)](i) increased to 140.3+/-13.0% (P<0.05 vs preischemic control 100%) after 5 min simulated ischemia, and remained at high level of 142.8+/-15.5% (P<0.05) after the following 15 min reperfusion. The increase in [Ca(2+)](i) during simulated ischemia and reperfusion was suppressed by 100 micromol/L amiloride (inhibitor of Na(+)/H(+) exchanger), 5 mmol/L NiCl2 (inhibitor of Na(+)/Ca(2+) exchanger) and calcium-free solution; [Ca(2+)](i) was 101.4+/-16.3%, 110.4+/-11.1% and 107.1+/-10.8%, respectively, after 5 min simulated ischemia, and 97.8+/-14.3%, 106.2+/-14.5% and 106.6+/-15.7%, respectively, after 15 min reperfusion. Compared with control cells, the amplitude of spontaneous calcium oscillation was lessened in cells treated with Ca-free perfusion and NiCl2 during reperfusion. In addition, no calcium oscillation was observed in cells pretreated with amiloride. These results suggest that Na(+)/H(+) exchange and Na(+)/Ca(2+) exchange are activated during simulated ischemia in isolated quiescent cardiac myocytes, leading to the elevation of [Ca(2+)](i) induced by simulated ischemia and reperfusion.

BmK I, an alpha-like scorpion neurotoxin, specifically modulates isolated rat cardiac mechanical and electrical activity / 生理学报

In this study, cardiotonic and cardiotoxic effects of Buthus martensi Karsch (BmK) I, a modulator of voltage-gated sodium channels, were investigated on the isolated rat hearts. The results showed that BmK I evoked complex effects characterized by a change in both cardiac mechanical and electrical activity. Langendorff perfusion showed that: (1) maximal left ventricular developed pressure (LVDP(max)) and dp/dt(max) were markedly increased by BmK I (0.5-10 micromol/L) in a dose-dependent manner (n=6, P<0.05), positive chronotropic effects were also induced by BmK I (n=6, P<0.05); (2) negative inotropic action and bradycardia could be elicited at a larger dose of BmK I (20 micromol/L); (3) the coronary flow varied inversely with the positive inotropic effects, coronary flow reduced during positive inotropic effects from 14.5 to 8.6 ml/min after administration of 500 nmol/L BmK I (n=6, P<0.05). In addition, tachycardia and complex cardiac arrhythmias were induced by BmK I (0.5-10 micromol/L). The modulating of BmK I on the heart mechanical, electrical activity could be partially recovered after washing. As propranolol was applied to block the release of catecholamines before administration of BmK I, suggesting that the changes in cardiac mechanical and electrical activity induced by BmK I might not due to catecholamine release from the nerve terminal and subsequent stimulation of the beta-adrenoceptor but attributable to the modulation of BmK I on cardiac voltage-gated sodium channels.

Comparison of radiography,CT and MR imaging in detection of arthropathies in patients with hemophilia / 中华放射学杂志

Objective To compare MR,CT,and radiography in the detection of arthropathies in patients with hemophilia.Methods Forty-one symptomic joint images in the 14 patients with hemophilia, aged from 11 to 24 years,were used in this study.Each joint had the examinations of radiography,CT and MR within one day.The severity of each joint was staged using conventional radiographic classification. Severe HA patients with stage 5 were excluded from the study.Imaging findings of soft tissue swelling, osteoporosis,epiphyseal overgrowth,joint erosion,cyst,joint space narrowing,bone marrow,joint effusion, hemorrhage,synovial hypertrophy,widened intercondylar notch as well as anterior and posterior crueiate ligaments(only for knee joint)were used for the all imaging comparison.Results The 41 symptomatic joints in 14 patients with hemophilia were classified by radiographic criteria into stage 0(n=5),stage 1(n=7),stage 2(n=6),stage 3(n=8)and stage 4(n=15).Soft tissue swelling or joint effusion was observed in 33 joints by radiographs,in 34 joints by both CT and MR.Joint erosions were demonstrated in 34 joints by MR,in 33 joints by CT and 20 joints by radiographs.Joint cysts were shown in 21 joints by MR,in 18 joints by CT and 9 joints by radiographs.Significant differences in detection of erosion and cyst were found between radiography with either CT(P0.05).MR showed improvement for detecting nlore loci of both erosion and cyst than CT and radiography,and also CT showed the improvement than radiography.Bone marrow edema 14 joints, hemon'hage in 34 joints and synovial hypertrophy in 27 joints were revealed on MR images.Conclusion MRI is superior to CT and conventional radiography in detecting the abnormal changes and should be considered as the first choice among the imaging modafities in evaluating hemophilic arthropathies.

Initial experiences about 16-detector row CT coronary angiography / 中华放射学杂志

70 bpm during scan),the proportion of segments that could not be assessed because of motion artifact were 0.1%(1/759),1.1%(7/649),2.5% (10/407),42.6%(103/242),and 75.5%(108/143),respectively.With conventional selective coronary angiography as the golden standard,the sensitivity,specificity,positive and negative prediction values to detect≥50% stenotic lesions in the assessable segments were 79.2%,96.0%,83.8%,and 94.6%,respectively.There was a significant correlation between the number of segments per patient not assessable because of motion artifact and heart rate during the scan(r=0.655,P=0.000).Conclusion MSCT is capable of achieving high accuracy for detection of coronary artery stenosis,and is a reliable technique to diagnose coronary artery disease.