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Chinese Journal of Natural Medicines (English Ed.) ; (6): 234-240, 2017.
Artigo em Inglês | WPRIM | ID: wpr-812119

RESUMO

The present study was designed to develop a sensitive and selective high performance liquid chromatography-tandem mass spectrometric method for the determination of Camellianin A in HepG2 cells. The extraction of Camellianin A was achieved using 15% trichloroacetic acid and then separated on a C column interfaced with a triple quadrupole tandem mass spectrometer in multiple reaction monitoring mode. The mobile phase was consisted of methanol-water (0.1% formic acid) (55 : 45, V/V). The total run time was 5.0 min. The method was linear in the concentration range of 0.25-250.0 ng·mL. The lower limit of quantification was 0.25 ng·mL. The intra- and inter-day relative standard deviations of entire concentration range were less than 9.3%. The proposed HPLC-MS/MS method was successfully applied to detect the intracellular concentration of Camellianin A in HepG2 cells.


Assuntos
Humanos , Cromatografia Líquida de Alta Pressão , Métodos , Flavonoides , Química , Células Hep G2 , Estrutura Molecular , Espectrometria de Massas por Ionização por Electrospray , Métodos , Espectrometria de Massas em Tandem , Métodos
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