Term structure, code formation and application tools of the clinical care classification system / 中华医学图书情报杂志

The term structure of the clinical care classification system(CCC) is consisted of nursing model, nursing components, nursing terms and term modifiers. Briefly described in this paper are its code formation, code genera-tion steps and application tools.

Comparison of hand hygiene compliance rates between doctors and nurses / 中国感染控制杂志

Objective To understand the current status of hand hygiene(HH) compliance among doctors and nurses,and strengthen the management of HH of health care workers(HCWs).Methods On-the-spot observation of HH implementation in clinical departments of the whole hospital was performed by a single-blind method,data were collected for statistical analysis.Results HH compliance rate of all clinical departments was 69.28％ (309/446),HH compliance rate of intensive care unit was the highest(87.93％),department of internal medicine was the lowest (63.03％),there were significant differences among different departments(P =0.001).Compliance rate of HH of nurses was higher than that of doctors(74.47 ％ vs 39.39 ％),difference was significant(P＜0.001);of different indications for HH,the lowest compliance rate was before contact patients(43.04％),HH of doctors after contact with patients and patients' surrounding were both lower than nurses,difference was significant(both P＜0.05).Conclusion HH compliance rate of doctors is lower than nurses,compliance rate of HH is the lowest before contact patients,awareness of HH among doctors and nurses before contact patients should be emphasized,targeted training should be conducted,thus reduce the incidence of healthcare-associated infection.

Construction and expression of non-structural protein gene 3-4b of HCV 1b based on the adenoassociated virus vector / 中华实验和临床病毒学杂志

<p><b>OBJECTIVE</b>To clone 1b type of HCV NS3-4b Gene and express in HEK 293 cells, lay the foundation for further study of the HCV NS3-4b recombinant adeno-associated virus vaccine and its dendritic cell vaccine.</p><p><b>METHODS</b>HCV 1b patients' serum was collected, and full length NS3-4b segment was amplified by RT-PCR and cloned into adeno-associated virus' expression vector pAAV. CMV. EGFP in order to express in HEK 293 cells. At last, it was validated whether express or not by Western Blot.</p><p><b>RESULTS</b>The 1b type gene NS3-4b were amplified and consistent to the expected size (2838 bp), the recombinant plasmid has been confirmed its successful restructured by double enzyme and sequencing, at last, Western Blot map can see objective protein expression after it transfect HEK 293 cells.</p><p><b>CONCLUSION</b>The adeno-associsted virus recombination HCV NS3-4b plasmid have successfully constructed and it can express in eukaryotic cells.</p>

Comparative study on the clinical characteristics of HBV infection patients with different pathologic inflammation grade / 中华实验和临床病毒学杂志

<p><b>OBJECTIVE</b>The aim of this study was to compare the biochemical and virological characteristics among patients infected with hepatitis B virus (HBV) according to pathologic inflammation grade.</p><p><b>METHODS</b>428 patients with chronic HBV infection accept liver biopsy, liver function test, HBeAg detection and HBV DNA levels detection. They were studied and subdivided into four groups according to pathologic inflammation grade. The biochemical and virological characteristics were studied. Univariate analysis was performed with the SPSS 16.0.</p><p><b>RESULTS</b>In different inflammation grading group, mean age and sex composition were no difference. Serum levels of ALT was highest in group G3 and lowet in group G0-1, there was statistically significant among groups (P = 0.005); AST and TBil were all highest in group G4 and lowest in group G0-1, statistically significant also found among groups (P = 0.000 & 0.004). Serum levels of ALB and PTA were all highest in group G0-1 and lowest in group G4, had statistically significant among groups (P = 0.000 & 0.000). There was no difference of HBV DNA level and percentage of HBeAg (+) among four groups (P = 0.565 & 0.065).</p><p><b>CONCLUSIONS</b>The serum AST, TBil, ALB and PTA were different and can partly reflect the inflammation degree of liver damage in patients with HBV infection. ALT and PTA can reflect the inflammation degree of G0-1, G2 and G3; AST, TBil, ALB and PTA reflect the G3 and G4. HBV DNA level and HBeAg status can not indicate the inflammation degree in HBV infection patients.</p>

The levels of HBsAg can predict the efficacy of peglated interferon-alpha 2a combined with adefovir dipivoxil, in HBeAg-positive chronic hepatitis B patients / 中华实验和临床病毒学杂志

<p><b>OBJECTIVE</b>To investigate the levels of HBsAg in predicting the efficacy of peglated interferon-alpha 2a combined with adefovir dipivoxil (ADV), in HBeAg-positive chronic hepatitis B patients.</p><p><b>METHODS</b>This trial enrolled 62 HBeAg-positive chronic hepatitis B patients with detectable HBsAg for at least 6 months prior to screening, serum HBV DNA levels of at least 100 000 IU/ml. The efficacy assessment: viral suppression below 100 IU/ml. The patients with HBV DNA < or = 100 IU/ml after 24 weeks therapy were divided into group A, in which monotherapy continued; While the rest were divided into group B, in which ADV was combined until week 48. In group B, at the end-of-treatment, the patients with HBV DNA < or = 100 IU/ml were divided into group B1, the rest were divided into group B2.</p><p><b>RESULTS</b>There was no significant difference on the baseline characteristics of patients between B1 and B2. There was significant difference on the levels of HBsAg at 12-week and 24-week between B1 and B2; while there was no significant difference on the levels of HBeAg.</p><p><b>CONCLUSIONS</b>The levels of HBsAg at 12-week and 24-week would be predictors to evaluate the efficacy of combined therapy in HBeAg-positive chronic hepatitis B patients.</p>

Expression of fibroblast activation protein in HBV related hepatocellular carcinoma / 中华实验和临床病毒学杂志

<p><b>OBJECTIVE</b>To analyze the gene expression level of fibroblast activation protein in HBV related hepatocellular carcinoma patients and discuss its clinical significance.</p><p><b>METHODS</b>FAP gene expression in 33 hepatocellular carcinoma patients cancer tissues, peficancerous tissues, distant relative normal liver tissues and 13 normal liver tissues were examined by reverse transcription PCR; and real-time fluorescent quantitative PCR (qRT-PCR) was used to quantify their expression.</p><p><b>RESULTS</b>FAP were expressed in all the tissues,the relative expression values in cancer tissues, peficancerous tissues and distant relative normal liver tissues were 5.14 +/- 6.69, 1.58 +/- 0.96, 1.63 +/- 0.94, respectively, the differences were statistically significant (F = 4.401, P < 0.05); and in TNM stage I, II, IIII, they were 2.89 +/- 3.35, 4.15 +/- 4.69, 10.09 +/- 9.51 respectively; in well-differentiated, differentiated and poorly differentiated hepatocellular carcinoma were 1.62 +/- 1.74, 3.84 +/- 3.79, 1.26 +/- 13.34 respectively. The differences were all statistically significant (P < 0.05).</p><p><b>CONCLUSION</b>FAP may play an important role in the occurrence and development of HBV related hepatocellular carcinoma.</p>