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1.
China Journal of Chinese Materia Medica ; (24): 1543-1547, 2015.
Artigo em Chinês | WPRIM | ID: wpr-351311

RESUMO

This research established an HPLC method for determination of six C-Glycoside flavones of warer-soluble total flavonoids from Isodon lophanthoides var. gerardianus (Benth.) H. Hara, and studied the antitumor activity of the warer-soluble total flavonoids. The HPLC system consisted of Kromasil 100-5 C18 (4.6 mm x 250 mm, 5 microm) column and a solution system of methanol, acetonitrile and 0.5% formic acid gradient elution at a flow rate of 0. 8 mL x min(-1) and the wavelength of detector was at 334 nm. The column temperature was 25 degrees C. The antitumor activity of water-soluble flavonoids was assayed using HepG2 cell as the tested cell. The linear ranges of vicenin II, vicenin III, isoschaftoside, schaftoside, vitexin, 6, 8-di-C-a-L-arabinosylapigenin were 0.25-2.53, 0.12-1.20, 0.37-3.69, 0.16-1.63, 0.19-1.92, 0.14-1.42 microg, respectively. The average recoveries (n = 6) were 99.6% (RSD 0.87%), 100.2% (RSD 2.0%), 99.6% (RSD 1.8%), 97.9% (RSD 1.5%), 98.8% (RSD 1.2%), 98.6% (RSD 1.2%), respectively. After exposure in 24, 48, 72 h, the total flavonoids showed inhibitory effect on the proliferation of HepG2 cells with IC50 as the evaluation index, the IC50 values of 1.89, 1.71, 1.51 g x L(-1), respectively. The method is quick, simple and accurate with good re- producibility, and can be used for determination of vicenin II, vicenin III, isoschaftoside, schaftoside, vitexin, 6, 8-di-C-a-L-arabino- sylapigenin in the warer-soluble total flavonoids from L lophanthoides var. gerardianus. The warer-soluble total flavonoids from L lophanthoides have inhibitory effect on the proliferation of HepG2 cells.


Assuntos
Humanos , Antineoplásicos Fitogênicos , Farmacologia , Linhagem Celular Tumoral , Proliferação de Células , Cromatografia Líquida de Alta Pressão , Medicamentos de Ervas Chinesas , Farmacologia , Flavonas , Farmacologia , Isodon , Química , Monossacarídeos , Farmacologia
2.
Chinese Traditional and Herbal Drugs ; (24): 3219-3223, 2014.
Artigo em Chinês | WPRIM | ID: wpr-854781

RESUMO

Objective: To investigate the water-soluble constituents of Isodon lophanthoides var. gerardianus. Methods: Compouds were isolated and purified by D-101, Sephadex LH-20, and ODS column chromatographies. The chemical structures were elucidated on the basis of spectral data and physicochemical properties. Results: Twelve compounds were isolated and identified as vitexin II (1), vicenin III (2), isoschaftoside (3), schaftoside (4), vitexin (5), 6, 8-di-C-α-L-arabinosylapigenin (6), apigenin-7-O-glucuronide (7), apigenin 6-C-β-L-arabin-osyl-8-C-α-L-arabinopyranoside (8), apigenin 6-C-β-D-xylopyranosyl-8-C-α-L-arabinopyranoside (9), caffeic acid (10), rosmarinic (11), and rutin (12). Conclusion: Compounds 1-9 are separated from I. lophanthoides var. gerardianus for the first time, and constituents of the water-soluble parts of I. lophanthoides var. gerardianus are studied systematically for the first time. Compounds 1, 6, and 11 exert antiproliferative effects on HepG2 cells.

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