RESUMO
The ethyl acetate part of the alcoholic extract of Cordia dichotoma fruits was purified by a combination of normal-phase silica gel column chromatography, Sephadex LH-20 gel column chromatography and semi-preparative HPLC, and the structure was identified by modern spectroscopic techniques (UV, IR, MS, NMR). A total of 10 compounds were isolated and identified as cordilide (1), (S)-2-hydroxy-3-(4′-hydroxyphenyl) propanoic acid (2), vanillic acid (3), p-coumaric acid (4), 3-hydroxy-1-(4-hydroxy-3-methoxyphenyl)propan-1-one (5), benzoic acid (6), p-hydroxypropiophenone (7), p-hydroxyacetophenone (8), 5′-methoxyevofolin B (9) and vanillin (10). Among them, compound 1 is a pair of new phenylpropanoid enantiomers, and compounds 3, 6, 8 and 9 were isolated for the first time from the genus.
RESUMO
Silica gel column chromatography, reversed phase C18 column chromatography, Sephadex LH-20 gel column chromatography, high performance liquid chromatography and medium performance semi preparative liquid chromatography were performed to separate and purify the chemical constituents of Hypericum lagarocladum N. Robson. Spectroscopic methods such as MS and NMR combined with physicochemical properties were applied in identifying the structures of the isolated compounds. A total of 11 compounds were isolated and identified as hyperlagarone A (1), hyperpatulone E (2), hyperbeanol G (3), uralione D (4), tomoeone F (5), pyramidatone A (6), tomoeone A (7), tomoeone B (8), hyperbeanol C (9), hyperbeanol A (10), and hypercohone G (11), respectively. Compound 1 is a new polycyclic polyprenylated acylphloroglucinol derivative, and compounds 2-11 are isolated from this plant for the first time. 11 compounds were tested for glucose uptake in L6 cells, and the results showed that compounds 7 and 8 had significant effect on glucose uptake.
RESUMO
This study was aimed to explore monocyte chemotactic protein 1 (MCP-1) and interleukin 8 (IL-8) expressions in human umbilical vein endothelial cells (HUVECs) affected by TNF-alpha and its molecular mechanism. RT-PCR assay was used to detect the expression of MCP-1 and IL-8 mRNA in HUVECs at various times following stimulation with TNF-alpha; the nuclear factor-kappa B (NF-kappaB) activation in HUVECs were detected by immunofluorescence. The results showed that after stimulation with NF-kappaB, the expression levels of MCP-1 and IL-8 mRNA in HUVECs increased, and changed along with different time; the expression of IL-8 mRNA reached to peak level at 8 hours and the expression of MCP-1 mRNA reached to peak level at 12 hours; the expression of NF-kappaB p65 protein began increasing at 30 minutes and reached to peak level at 1 hour; then the staining of cytoplasm gradually weakened, while staining of nuclei was enhanced, which indicated significant nuclear translocation of NF-kappaB p65. It is concluded that the TNF-alpha induces expressions of IL-8 mRNA, MCP-1 mRNA and NF-kappaB in HUVECs, and NF-kappaB activities signal pathway may play a role in IL-8 mRNA and MCP-1 mRNA expressions.