Progress in genetic and epigenetic research on in-stent restenosis after percutaneous coronary interventions / 中华医学遗传学杂志

Coronary heart disease is one of the most important causes of death in human, and consumes vast medical resources. Percutaneous coronary intervention (PCI) has been a significant breakthrough for its treatment. However, clinical application has been hampered by in-stent restenosis (ISR). Although drug eluting stent (DES) has reduced the occurrence of restenosis, incidence of ISR is still about 5% to 10%. The main reasons for restenosis after PCI are hyperplasia of vascular endothelial cells and smooth muscle cell migration. The exact mechanism of personalized differences in restenosis is not clear yet, but there may be a variety of risk factors. In addition to aging, smoking and diabetes, an increasing number of studies have found that genetic and epigenetic factors play an important role in ISR. In this article, authors have reviewed genetic and epigenetic factors on the progression of ISR, which may help to determine the genetic risk factors in patients with ISR after PCI.

Kinetic turbidimetric limulus assay of bacterial endotoxin in the replacement solution of on-line hemodiafiltration / 南方医科大学学报

<p><b>OBJECTIVE</b>To determine bacterial endotoxin in the replacement solution of on-line hemodiafiltration (on-line HDF) using kinetic turbidimetric limulus test.</p><p><b>METHODS AND RESULTS</b>Validation test was performed with the replacement solution of on-line HDF in which quantified standard endotoxin was added. The recovery rates of endotoxin from the replacement solution and its dilutions at 1/5, 1/10, and 1/20 were 58.17%, 106.7%, 99.00% and 98.79%, respectively, suggesting that the optimal dilution was at 1/10. Standard endotoxin was added into the replacement solution of on-line HDF of 3 batches (040408, 040511,040527), and the recovery rates in their dilution at 1/10 were 76.32%, 99.00% and 96.24%, respectively. The standard endotoxin in the working curve was 1.00, 0.125, and 0.0156 Eu/ml (endotoxin unit/ml), and the dilution at 1/10 of the replacement solution is effective to eliminate the interference in limulus test.</p><p><b>CONCLUSION</b>Kinetic turbidimetric limulus test provide a means to detect endotoxin in the replacement solution of on-line HDF.</p>

Application of the kinetic turbidimetric limulus test to ceftazidime injection / 中国药理学通报

AIM: The kinetic turbidimetric Limulus test was used in the endotoxin assay of Ceftazidime injection. METHODS: The validation test was fulfiled, when the amount of endotoxin spiked in Ceftazidime injection sample (Lot. No. 010202) in which concentration of its dilution solution were 10, 5,2,1,0.5 g·L-1. They were recovered in 60.08%, 82.90%, 85.22%, 94.00%, 92.67%; with the amount of endotoxin spiked in Ceftazidime sample (Lot. No. 010103, 010104, 010202), their 1 g·L-1 dilution were recovered in 109.9%, 101.7% and 86.56%. RESULTS: With standard endotoxin working curve is 5.00, 0.500, 0.0500 ZU·L-1. The 1 g·L-1 solution to Ceftazidime was effective to eliminate the interference in Limulus test. CONCLUSION: The kinetic turbidimetric Limulus test provide a new way to the detection of endotoxin in Ceftazidime injection.