EvaluationofMRperfusionweightedimagingofthebrainchronicmountainsickness / 实用放射学杂志

Objective Toinvestigatethehemodynamicchangesofthegraymatterandwhitematterinpatientswithchronicmountain sickness(CMS).Methods 14CMSpatientsand12healthyvolunteerswererecruitedinthisstudy.Allofthem wereperformedbyroutineMRI andperfusionweightedimaging(PWI)scans.Aftertheappropriatepost-processing,therelevanthemodynamicparametersofdifferent graymatternucleiandwhitematteroffrontallobewereobtained,andthedifferenceofeachvaluebetweenthetwogroupswascompared.Results Therelativecerebralbloodflow (rCBF)decreasedinthebraingraymatterandwhitematterinCMSpatientswhencomparedwith normalgroup(P<0.05)[rCBFofthespleniumofthecorpuscallosum was(40.57±18.89)mL·100g-1·min-1forCMSpatients, and (64.56±18.55)mL·100g-1·min-1fornormalgroup],andatthesametime,meantransittime (MTT)andtimetopeak (TTP)werebothprolonged(P<00.5)[MTTofthespleniumofthecorpuscallosumwas(66.3±19.5)sforCMSpatients,and(48.0±2.17)sfor normalgroup;TTPofthespleniumofthecorpuscallosum was(26.86±4.67)sforCMSpatients,and (23.17±3.01)sfornormal group].Conclusion Collateralcirculation mayexistinCMSpatients’brain.Therearecerebralhemodynamicdifferencesbetween CMSpatientsandhealthyvolunteers.MRPWIisanimportantwayfordiagnosisofCMS.

The imaging features of osseous metastasis of hepatic alveolar hydatida / 中华放射学杂志

Objective To investigate the CT and MR imaging feature of osseous metastasis of hepatic alveolar hydatida.Methods CT and MR imaging features of osseous metastasis of hepatic alveolar hydatid were retrospectively analyzed in 14 cases confirmed by clinic and radiology. Results Of the 10 vertebral metastasises,three vertebral bodies were involved in 6 cases,two adjacent vertebral bodies were involved in 2 cases, single vertebral body was involved in 1 case, corresponding vertebral accessory was involved in 9 cases,irregular mass was formed in paravertebral muscles with ill-defined margins in 9 cases, adjacent ribs were involved in 6 cases. On CT scans, the lesions of vertebral body showed osteolytic and geographical bone destruction with bone sclerosis. The lesions of vertebral accessory showed swelling and osteolytic bone destruction, small cystic density and calcification of cystic wall and irregular calcification were found in 7 cases.The lesions of scapula,costal cartilage,ribs and pelvis showed swelling and osteolytic bone destruction,and bone sclerosis,and revealed cystic density with calcified speckles.On MR scans,the lesions of vertebral body and paravertebral muscles showed high and low mixed signal,with hypointensity on T1WI and T2WI, vertebral accessory and adjacent ribs were involved in 6 cases. Small cyst with T2 hyperintensity was found in 4 cases. The lesions of ribs and pelvis showed swelling and osteolytic bone destruction and adjacent medullary appeared hypointensity on T1WI and T2WI, cystic signal of T2 hyperintensity was identified. There were 5 cases of pulmonary metastasis and 6 cases of retroperitoneal metastasis. Conclusion Imaging features of osteolytic,geographical bone destruction with bone sclerosis, small cystic density or cystic signal and calcified speckles or arc calcification in mass are helpful for the diagnosis of osseous metastasis of hepatic alveolar hydatid.

Study on the characteristics of"indulgence of vein"in patients with hepatic alveolar echinococcosis / 实用放射学杂志

Objective To explore the physiological characteristics of vascular invasion of hepatic alveolar echinococcosis(HAE) and the growth pattern of lesion.Methods 120 cases with HAE were all scanned by 256 slices MSCT,then the images were taken three-dimensional reconstruction.A comparison between pathology and images were made for the evaluation of vascular invasion.Results Pathological examination showed that the invasion rate of intrahepatic vein(left hepatic vein,middle hepatic vein,right hepatic vein, inferior vena cava),portal vein and hepatic artery were 34.38%,31.11%,22.50%,respectively.176 branches of the intrahepatic vein, 67 branches of the hepatic artery and 127 branches of portal vein were involved.Combined with the pathology,the Kappa values were 0.868,0.725 and 0.844.Conclusion HAE is easy to involve the intrahepatic veins as it grows,considering its"indulgence of vein"feature.In order to improve the targeting effect,the mode of administration could be changed.MSCT can exactly evaluate the invasion of intrahepatic vessels,providing important basis for clinical treatment.

The value of DWI in hepatic alveolar echinococcosis growth activity in children / 实用放射学杂志

Objective To assess the value of magnetic resonance diffusion weighted imaging(DWI) and apparent diffusion coefficients(ADC) values of hepatic alveolar echinococcosis(HAE) in children.Methods 20 cases of children(≤14 years) with HAE were collected in this restrospective study.PNM staging was determined, the HAE peripheral area of DWI lesions with different P stages was observed, and the ADC value of the peripheral area was measured.The comparison of alveococcus lesions in different stages of DWI with continuous edge degree and ADC value difference was done to evaluate the growth activity.Results There were 5 cases of P1 lesions, 7 cases of P2 lesions, 2 cases of P3 lesions and 6 cases of P4 lesions.DWI features of peripheral area were as follows: High signal ring band between HAE lesion edge and adjacent normal hepatic parenchyma was observed.P1 lesions showed almost complete obviously high signal peripheral area, indicating the most active proliferation, P2 and P3 lesions of peripheral area were continuous and with high signal, and still had obvious growth activity.P4 lesions of peripheral area were not continuous, while the signal decreased, indicating the activity also decreased.The highest ADC value was detected in P1 lesions group of and the ADC value of P2 lesions group were lower than P1, and the ADC value of P4 lesions group were the lowest.P3 lesions samples were too small and thus no statistical analysis was done.Differences of ADC value between the three groups were statistically significant (P<0.05).Conclusion DWI image features could be used to assesse the growth activity of HAE in children with different stages to a certain extent.ADC values measurement provides important reference value for evaluating the growth activity at various stages of the lesions.

MR signal and spectroscopy analysis of lumbar spine bone marrow in Tibetan / 实用放射学杂志

Objective To investigate the MR signal intensity and spectroscopy characteristics of lumbar bone marrow in normal adult of Tibetan and Han nationality in high altitude.Methods According to the inclusion criteria,lumbar MRI examinations in Tibetan and Chinese volunteers(each 50 cases)were obtained.For each inspector,the lumbar 3 vertebra was selected,TFE sequence was used to measure the signal strength,T2map was used to measured the T2time,and 1H-MRS was used to measure the lumbar bone marrow spectrum signal.The measured data were statistically analyzed.Results Compared with the Han nationality in Xining area,T1-TFE sequence of Tibetan showed obvious low signal in the lumbar vertebra.The relative signal intensity were significantly different(P=0.001).For measurement of T2time,there was no significant differences(P=0.061).The spectrum analysis showed a line of low fat high water for Tibetan,and a line of low water high fat for Han nationality in Xining area.There were significant differences for water peak intensity,Width, Height and peak Area,but no significant differences in Lipid peak.There were significant differences(P<0.05)on Lipid water absorption ratio, fat water ratio,fat fraction between the two groups.Conclusion The Tibetan shows a low signal on T1WI sequence of lumbar spine which is considered in hypoxia condition for a long time,and the bone marrow red pulp associated with water content increased sig-nificantly.

Adaptive modulation of brain in normal adult living in plain to high altitude areas:studies on resting-state fMRI / 实用放射学杂志

Objective To explore the adaptive modulation of brain in structure and function when moving to high altitude areas based on voxel-based morphometry (VBM),amplitude of low frequency fluctuation (ALFF)and regional homogeneity (ReHo) method.Methods Resting-state functional magnetic resonance imaging (fMRI)of brain was respectively performed in 1 9 normal adults who moved to high altitude areas within a week,and they have matched age and education level.The subj ects were performed the same scanning after one year.The VBM,ALFF and ReHo methods were adopted to analyze the functional MRI data.Results There were no significant differences in brain structure between the two groups.There were significant differences in brain function in resting state.Subj ects staying in high altitude areas for one year had increased ALFF in left middle frontal gyrus,decreased ALFF in right lingual gyrus(P<0.01),increased ReHo in the left orbital frontal gyrus,and decreased ReHo in right cuneus and right superior parietal gyrus(P<0.05).Conclusion There are significant changes in brain function of normal adult after stayed in high altitude for one year,which might be associated with long-term chronic hypoxia.

Application value of the diffusion-weighted imaging in combination with dynamic enhanced MRI in detection of peripheral zone of hepatic alveolar echinococcosis / 实用放射学杂志

Objective To explore the application value of the diffusion-weighted imaging (DWI)and dynamic contrast-enhanced MRI in detection of peripheral zone of hepatic alveolar echinococcosis (HAE).Methods Twenty patients with HAE underwent abdominal plain scan,DWI and dynamic enhanced MRI.The ADC values and enhanced ratio of the central necrosis,solid component,peripheral zone and normal liver parenchyma were measured at a workstation,and further were analyzed and compared.Results 32 lesions were detected in 20 patients with HAE.Two groups were divided according to the lesion with central necrosis (group A)or not (group B).In group A, the ADC values of the solid part and normal liver parenchyma were (1.52±0.30)×10 -3 mm2/s and (1.22±0.30)×10 -3 mm2/s. Meanwhile,in group B those were (1.50±0.25)×10-3 mm2/s and (1.03±0.28)×10-3 mm2/s,exhibiting no statistical differences between the two groups.In group A and B,the ADC values of the peripheral zone of lesion were 1.34(1.10,1.61)×10 -3 mm2/s and 0.96 (0.86,1.22)×10 -3 mm2/s,exhibiting statistical differences (Z =2.867,P =0.004);meanwhile,the enhanced ratio were 1.70± 0.36 and 1.58±0.30,exhibiting no statistical difference.No correlation between ADC values and enhanced ratio was found in the peripheral zone.Conclusion DWI at 3.0T can display visually the HAE,and the ADC value may reflect the DWI features of periph-eral zone.The ADC values in combination with enhanced ratio may help for the diagnosis.

CT in Measurement Hepatic Hemodynamics and Volume for Adults Living at Different Altitudes / 中国医学影像学杂志

Purpose Changes of hepatic perfusion and volume of normal adults living at different altitudes are investigated with hepatic perfusion imaging using multi-slice spiral CT to explore the perfusion status of normal human liver in hypoxia condition, and to provide the basis for the diagnosis and treatment of liver diseases. Materials and Methods Sixty volunteers were divided into three groups according to altitude:22 cases in group A with altitude from 2260 to 3100 meters, 20 cases in group B with altitude from 3260 to 4100 meters, and 18 cases in group C with altitude from 4260 to 5260 meters, time-density curves within the ROI was drawn with hepatic hilar region as the center, and hepatic artery perfusion (HAP), portal vein perfusion (PVP), total hepatic perfusion (TLP), hepatic arterial perfusion index (HPI) were calculated using deconvolution method;and point circle method was used to measure the volume of liver manually. Results HAP of group A, B, C were (10.50±3.62) ml/(100 ml · min), (15.03±4.27) ml/(100 ml · min) and (18.39±7.20) ml/(100 ml · min) respectively, and there was significant difference between group A and group C (P<0.05);PVP of three groups were (138.78±14.29) ml/(100 ml · min), (80.42±16.80) ml/(100 ml · min), and (63.78±7.79) ml/(100 ml · min) respectively, signiifcant differences (P<0.05) can be detected for the comparison between each two of them; TLP of the three groups were (149.30±15.55) ml/(100 ml · min), (95.57±18.75) ml/(100 ml · min) and (82.19±10.56) ml/(100 ml · min) respectively, statistically signiifcant differences (P<0.05) can also be found for the comparison between each two of them. HPI of the three groups were 7.00±2.17, 16.27±4.22 and 22.05±7.90 respectively, statistically signiifcant differences (P<0.05) were observed for the comparison between each two of them;liver volume of the three groups were (1173.5±155.2) cm3, (1282.9±362.2) cm3 and (1525.4±352.2) cm3, difference between group A and group C was statistically signiifcant (P<0.05). Conclusion Liver perfusion can be affected by altitude, with the altitude increasing, HAP and HPI will also raise gradually, while PVP and TLP will decrease accordingly, indicating that hypoxia environment due to high altitude will do certain damage on the liver. MSCT perfusion imaging is able to relfect the hemodynamic changes of liver, thus is useful in clinical diagnosis and treatment of liver diseases.

Effects of bone marrow mesenchymal stem cell conditioned medium on bioactivity of scar fibroblasts / 中国组织工程研究

BACKGROUND:Mesenchymal stem celltransplantation promoted skin repair in trauma via various regulatory mechanisms and inhibited scar formation. At present, many scholars believed that bioactive factors secreted by mesenchymal stem cells played an important role. OBJECTIVE:To investigate the effects of bone marrow mesenchymal stem cellconditioned medium on the proliferation and col agen synthesis of hypertrophic scar fibroblasts. METHODS:Human bone marrow mesenchymal stem cells and hypertrophic scar fibroblasts were isolated and cultured, and bone marrow mesenchymal stem cellconditioned medium was prepared. Hypertrophic scar fibroblasts were cultured in vitro with 12, 24, and 48 hour-col ected conditioned medium for 24 hours, which was compared with blank control group. The proliferation of cells was determined by CCK-8. Type I and type III col agen expression in hypertrophic scar fibroblasts was detected using real-time PCR. RESULTS AND CONCLUSION:Compared with the blank control group, 24 and 48 hour-col ected conditioned medium significantly inhibited the proliferation of hypertrophic scar fibroblasts (P<0.01), and also suppressed col agen synthesis of hypertrophic scar fibroblasts (P<0.01). Results suggested that bone marrow mesenchymal stem cellconditioned medium inhibited the proliferation and col agen synthesis of hypertrophic scar fibroblasts by secreting anti-fibrotic bioactive factors, which may provide new theoretical supports for celltherapy to reduce cutaneous scarring.

Effect of Panax quinquefolium saponins from steams and leaves on acidosis and the metabolism of free radical in focal cerebral ischemia injury rats / 中国基层医药

Objective To investigate the protective effct of Panax quinquefolium saponins from steams and leaves(PQS)on focal cerebral ischemia injury in rats and its mechanisms. Methods Wistar rats were randomly divided into sham operation group,model control group,nimodipine group and two PQS groups,in which PQS of 100 and 50 mg/kg was intragastrically administered. Focal cerebral ischemia model was established by middle cerebral artery occlusion (MCAO)in rats, via string ligation of artetia carotis interna. The content of malondicldehy de(MDA) was determined by thibabituric acid ( TBA ) test, the activity of lactate dehydrogenase ( LDH ), superoxide dismutase (SOD)and the content of lactic acid(LA) were detected by chemical colorimetry test in cerebral tissues. Results PQS( 100,50mg/kg)could significantly decrease the content of LA、MDA and increase the activity of LDH、SOD. Conclusion The protective mechanism of PQS on focal cerebral ischemia injury may be related to reduce acidosis, anti-free radical and resist oxidative damage.

Genetic polymorphism of 6 short tandem repeat loci in Chinese korean / 中国组织工程研究

BACKGROUND: Many scholars have reported the study of genetic polymorphism of short tandem repeat loci in different countries, areas, and population. But what is the genetic polymorphism of short tandem repeats loci of Chinese Korean is still unknown.OBJECTIVE:To investigate the distribution of the genetic polymorphism of D16S539 D7S820, D13S317, CSF1PO, TPOX and TH01 in Chinese Korean, obtain the crowded genetic data of corresponding polymorphism loci.DESIGN: Single sample observation.SETTING: Staff Room of Biology, Department of Detecting and Analyzing DNA, Mudanjiang Medical College.MATERIALS: Periphoral blood samples from 100 unrelated individuals in Korean of Mudanjiang were chosen from January 2001 to February 2001.METHODS: Genotype detection was performed in one hundred unrelated individuals with polymerase chain reaction amplification fragment length polymorphism analysis.MAIN OUTCOME MEASURES: Sample genotype of D16S539,D7S820, D13S317, CSF1PO, TPOX, THO1 6 short tandem repeat loci.TH01 locus.CONCLUSION: The genotypes distributions of the 6 short tandem repeat (STR) in Chinese Korean met Hardy-Weinberg equilibrium, and have higher heterozygosities. The gene frequency and other data obtained can provide the basis for studying the crowded genetics of Chinese Korean.

Genetic polymorphism of 6 short tandem repeat loci in Chinese Korean / 中华医学遗传学杂志

<p><b>OBJECTIVE</b>To analyze genetic polymorphism of D16S539 D7S820 D13S317 CSF1PO TPOX and TH01 in Chinese Korean.</p><p><b>METHODS</b>One hundred unrelated individuals were analyzed by PCR amplification fragment length polymorphism analysis.</p><p><b>RESULTS</b>Six alleles and 18 genotypes of D16S539 locus, 7 alleles and 22 genotypes of D7S820 locus, 7 alleles and 23 genotypes of D13S317 locus, 6 alleles and 16 genotypes of CSF1PO locus, 6 alleles and 11 genotypes of TPOX locus, 5 alleles and 12 genotypes of TH01 locus.</p><p><b>CONCLUSION</b>The genotype distribution of all the 6 short tandem repeat(STR) in Chinese Korean met Hardy-Weinberg equilibrium, and have higher heterozygosities. The data obtained can be used in human identity and paternity testing, and in other genetic researches and population investigation.</p>

CT and MRI Appearances of Eclamptic Encephalopathy in Plateau / 实用放射学杂志

Objective To study CT and MRI findings of the eclamptic encephalopathy in plateau.Methods CT and MRI findings in 28 patients in plateau with eclamptic encephalopathy were retrospectively analyzed.Results CT and MRI appearances of eclamptism were as follow:normal in brain in 3 cases;cerebral edema in 18 cases,including slight encephaledema in 2 cases,focal encephaledema in 11 cases and diffuse encephaledema in 5 cases;cerebral hemorrhage in 6 cases and sinovenous thrombosis in 1 case.Conclusion CT and MRI scan are of significant value in diagnosis and treatment of eclamptic encephalopathy in plateau.

A Report of X-ray Manifestations of the Hereditary Multiple Osteochondroma:21 Patients in 5 Pedigree / 实用放射学杂志

Objective To investigate the X-ray and clinic manifestations of hereditary multiple osteochondroma(HMO).Methods Five patients with HMO clinically and pathologically confirmed and 63 persons in their pedigree were studied.Results Of 63 cases inquired in this study,there were 21 patients with hereditary multiple exostosis,including 16 men and 5 women.In 5 cases confirmed pathologically,4 patients had hereditary history of pedigree and the members of three generation in 1 patient with HMO had no the history of pedigree.Conclusion HMO has the remarkable sex difference and the typical inherited trend including the location of exostosis.HMO has inherited character of skipping generation.

CT Diagnosis of Hepatic Alveolar Echinococcosis / 实用放射学杂志

Objective To discuss the CT features and pathologic basis of hepatic alveolar echinococcosis. Methods Total 19 cases were confirmed by ELISA and IHA of antibody of echinococcosis. A part of them were confirmed by pathalogy. Results The main features of the disease were irregular,obscure contour and heterogeneous density with mainly hypodense in the liver. Almost of all accompanied with calcification. The calcification was showed some certainly signification. No contrast enhancement was observed within the lesions after administration of intravenous contrast medium. There were three type of this disease based on CT features including map type, halo type and pseudocystic type. Conclusion CT scanning has value of not only for diagnosis of hepatic alveolar echinococcosis but also useful to display each complications and metastasis.

CT Diagnosis of Tuberculosis of Brain / 实用放射学杂志

Objective To explore the diagnostic and classified value of tuberculosis of brain with CT imaging.Methods CT scans were performed in 37 cases with tuberculosis of brain confirmed by clinic and laboratory examination of CSF.Results CT images were abnormal in 26 cases.6 cases were the type of brain substance,6 cases were meningeal type and 14 cases were mixed type.Conclusion CT examination can offer correct evidences of location and qualitalive diagnosis of tuberculosis of brain .