Inj ury of male reproductive system of rats by prepubertal exposure to exogenous estrogen and its natural process of tissue repair / 吉林大学学报(医学版)

Objective To investigate the influence of prepubertal exposure to estradiol benzoate (EB)in the male reproductive system of the rats and the natural process of tissue repair,and to clarify the possible mechanism of the reproductive toxicity of exogenous estrogen.Methods Ninety 2 1-day-old male Wistar rats were randomly divided into 2 experimental groups (low dose of EB group and high dose of EB group,n=30)and control group (n=30). The rats in the experimental groups were injected with EB dissolved in peanut oil at 15(low dose of EB group)and 15 000μg·kg-1 (high dose of EB group)respectively,the rats in control group received equal vehicle injection only,once every other day for two weeks from postnatal day(PND)21 to 34.All of them were normally fed after the drug usage was stopped.The testes were harvested at the stages of PND 60 and PND 125(n=15 at each stage).The serum levels testosterone of (T),follicle-stimulating hormone(FSH),luteinizing hormone(LH),prolactin (PRL)and estradiol(E2)of the rats in various groups were detected with radioimmunology method and the weights of the rats in various groups were recorded;the histological changes of the testes tissue were observed with light microscope.Results On PND60,compared with control group,the T levels in low dose of EB group and high dose of EB group were decreased(P0.05);the weights of testes were decreased(P0.05 ), and the weight of testes was decreased (P0.05),and the weight of testes was decreased(P<0.01);there was still no sperm in high dose of EB group, the number of sperms was increased in low dose of EB group, but it was still lower than that in control group.Conclusion EB is harmful to the reproductive system and can change the normal serum sex hormone levels,even induces the irreversible injury.

Establishment of scale of death attitude for nurses and testing of its validity and reliability / 中国实用护理杂志

ObjectiveTo design the scale of death attitude for Chinese nurses and evaluate its validity and reliability.MethodsThe scale draft was formed through the literature review,the formal scale was got via expert consultation and interview survey of 20 cases of small sample.120 clinical nurses were selected for investigation.Then item analysis,factor analysis,correlation analysis and reliability analysis were applied to establish the scale′s validity and reliability.ResultsThe nursing staff death attitude scale consisted of 25 items,the varimax rotation factor analysis of the scale identified seven principal factors and explained for 63.732％ variances.The correlation coefficient between each item and its domain was 0.498～0.808,the Cronbach′s α coefficient of five domains was 0.595～0.774,the Cronbach′s α and split-half coefficient for the whole scale were 0.824 and 0.868 respectively.ConclusionsThe scale has high reliability and validity,and it can be the effective test instrument for death attitude of nurses in China.

Validation of a Chinese version of Death Attitude Profile-Revised / 中国实用护理杂志

Objective To develop a Chinese version of Death Attitude Proevaluate its reliability and validity by applying it in the Chinese Christians.Methods After undergoing forward and backward translation of the DAP-R by expert consultation and in-depth interview,150 Christians over 40 years old were recruited to complete the Chinese version of DAP-R,And then factor analysis,correlation analysis and reliability analysis were applied to establish the scale's construct validity and reliability.Results The final scale consisted of five dimensions with 25 items; the varimax rotation factor analysis of the scale identified seven principal factors and explained for 67.075% variances; the correlation coefficient between each item and its domain was 0.432～0.850; Cronbach's α coefficient of five domains were 0.585～0.853,the Cronbach's α and split-half coefficient for the whole scale were 0.840 and 0.843 respectively.Conclusions The Chinese version of DAP-R possesses acceptable validity and reliability in the setting of Christians,and more in-depth study on the scale needs to be done in a wider population.

Behavior and Exposure to Violence:Mediating Role of Self-esteem in Rural Middle School Students / 中国临床心理学杂志

Objective:To test a model in which self-esteem mediated the effects of behavior on exposure to violence(victimization) in a sample of rural middle school students.Methods:A cross-section study was conducted in 3620 middle school students randomly selected from rural areas.A questionnaire of violence(WHO) and Achenbach Children Behavior Checklist(CBCL) and Rosenberg self-esteem scale were used to measure the exposure to violence behavior,and self-esteem respectively.Results:Hierarchical regression analyses provided evidence that the addition of self-esteem reduced the links between behavior and exposure to violence(? from 0.232 to 0.226).Sobel Z-test predicted that self-esteem partially mediated the effects of behavior on exposure to violence(victimization) with significant difference(? from 0.232 to 0.226,Sobel-Z value=2.340,P=0.019).Speech violence,physical violence,and sexual violence were tested by the same model respectively,and the results showed that self-esteem partially mediated the effects of behavior on speech violence with significant difference(? from 0.225 to 0.200,Sobel-Z value=1.992,P=0.046),but not on physical or sexual violence.Conclusion:Self-esteem partially mediates the effects of behavior on exposure to violence(emotion violence).