Emodin alleviated kidney damage in high fat diet induced obese mice / 中华内分泌代谢杂志

Objective To explore the protective effect of emodin on kidney damage in high-fat diet-induced obese mice. Methods Male C57bl/ 6 mice were divided into groups according to diet and treatment. Collagen Ⅳ(Col4), phosphatidylinositide 3-kinase(PI3K), phosphorylated phosphoinositol 3-kinase(p-PI3K), protein kinase (Akt), and phosphorylated protein kinase(p-Akt) were measured by Western blotting method. Results Col4 was increased, while p-PI3K and p-Akt were decreased in kidney tissue in high-fat diet-induced obese mice. However, there was lower level of Col4, but higher levels of p-PI3K and p-Akt in kidneys. Conclusion Kidney damage of high-fat diet-induced obese mice seems to be alleviated by emodin via improving insulin sensitivity.

Association between adverse obstetric outcomes and abnormal maternal serum markers in the second trimester screening / 上海交通大学学报（医学版）

Objective · To evaluate the association between the abnormal maternal serum markers of alpha fetoprotein (AFP), human chorionic gonadotrophin (hCG) and unconjugated estriol (uE3) in the second trimester screening and the adverse obstetric outcomes other than trisomy 21 (T21),trisomy 18 (T18) and open neural tube defects (ONTD), and to provide local data for supporting evidence based clinical managements. Methods · A retrospective cohort study was performed in the women who received second trimester maternal serum screening in the International Peace Maternal and Child Health Hospital between 2012 and 2014, with naturally conceived singleton pregnancies. Obstetric outcomes were followed up by searching electronic medical records within the hospital. Abnormal level of marker was defined as a MOM value ≥ 99th (P99) or ≤ 1st percentile (P1) of the overall screened population. Incidence of an adverse obstetric outcome was compared between the groups with abnormal markers and the control with all markers in normal. Results · ① A total of 25616 pregnancies were included in this study, in which 4526 were identified as having various adverse obstetric outcomes. Among them 4143 pregnancies were with isolated and 383 pregnancies were with co-occurring two or more adverse outcomes. ② When compared to pregnancies with normal levels of all three serum markers, pregnancies with decreased AFP or decreased hCG did not show associations with any adverse obstetric outcomes. However, pregnancies with increased AFP, increased hCG or decreased uE3 were at increased risk for a variety of abnormal pregnancy outcome. In 18 pregnancies with an outcome of fetal chromosomal abnormalities other than T21 and T18, 9 presented with either increased AFP, increased hCG or decreased uE3, with relative risk ratios of 13.33、35.00 and 59.00, respectively. ③ The performance of those markers tended to be improved in a subset of adverse obstetric outcomes, including low birth weight

Clinical, myopathological and genetic research of a patient with distal myopathy caused by caveolin-3 deficiency / 中华神经科杂志

Objective To report the clinical,myopathological and genetic features of a patient with distal myopathy caused by caveolin-3 (CAV3) deficiency.Methods The patient was a 27-year-old female.She had an onset symptom of asymmetric lower extremities weakness.The proximal limb-girdle muscles were involved subsequently.Clinical data of this patient were collected.The leg muscle magnetic resonance imaging (MRI) and an open biopsy of left tibialis anterior muscle were performed.In addition to histological,enzyme histochemical staining and ultrastructural examination,immunohistochemical staining with antibody against CAV3 was done.CAV3 gene was analyzed in the patient and her parents.Results Tl-weighted enhanced skeletal muscle MRI of the lower limbs showed the abnormal signal in distal and proximal muscles.Muscle biopsy showed moderate dystrophic changes and immunostaining for CAV3 showed reduced plasmalemma in the muscle fibers.Gene analysis disclosed a heterozygous c.136G ＞ A (p.Ala46Thr)mutation in the CAV3 gene,and the patient's parents did not have this mutation.Conclusions We report a distal myopathy case caused by c.136G ＞ A (p.Ala46Thr) mutation in the CAV3 gene,who had an onset symptom of asymmetric lower extremities weakness.The proximal limb-girdal muscles were also involved.This would help clinical doctors to know more about this rare myopathy.

Strengthening the CCP Party building of public hospitals leveraging CCP Party building performance evalua-tion / 中华医院管理杂志

To meet the needs of serving the general strategy,the central work and hospital development,the CCP committee of Beijing Municipal Administration of Hospitals has innovated and improved the leadership of the CCP building,and promoted the model at public hospitals by formulating and announcing the Indicators System for Municipal Public Hospitals ’ Party Building Performance Evaluation.This implementation aims to to guide,regulate and encourage the CCP work,which proves effective enhancement of the CCP building in municipal hospitals in terms of quality,efficiency,and effectiveness.

Studying on the expression of P27,c-fos protein and DWI imaging after cerebral ischemia and reperfusion / 中国基层医药

Objective To study the expression of P27,c-fos protein in neuron cell after ischemia and reperfusion accompany with the DWI imagings in rat brain.Methods 12 healthy SD rats with MACO were divided into three groups.Group A,ischemia 30 minutes with 60 minutes reperfusion;Group B,ischemia 60 minutes with 60 minutes reperfusion.Group C,ischemia 60 minutes.The DWI imaging was scanned after surgery immediately.The expressions of P27 and c-fos were determined by immunochemistry procedure.Results The P27 protein positive reaction was not detected in all ischemia and reperfusion groups.The c-fos protein positive reaction was observed in the cortex and basal ganglion in all groups.The hyperintense signal was presented the in DWI after fifteen minutes following the ischemia.Conclusions P27 protein is endogenous cyclin dependent kinase inhibitor.There may be the relationship between low expression of P27 protein in rat brain and the programmed cell death in neuron after the ischemia.C-fos protein participates in the neuron cell injury after the ischemia.DWI has good sensitivity to acute cerebral ischemia.

Clinical and experimental pathology of Moyamoya disease / 中华医学杂志(英文版)

<p><b>OBJECTIVE</b>To investigate the etiology, pathology, and mechanism of pathogenesis of Moyamoya disease.</p><p><b>METHODS</b>A total of 15 human autopsies were analyzed. In addition, in order to create an animal model of the disease, 21 Japanese rabbits were divided randomly into two groups and subjected to injections of horse serum either intravenously or locally in the area of the sympathetic ganglia. Pathological and immunohistochemical characteristics were observed.</p><p><b>RESULTS</b>The pathological features of the autopsies and the animal models both involved intima hyperplasia and stenosis or even occlusion of the lumen in the terminal ends of the internal carotid artery and the anterior and middle cerebral arteries. Disconnections or even breakages of the inner layer of the lumen were also observed, without an obvious inflammatory response. Hyperplasic smooth muscle cells of the medial membrane had extended inward through broken portions of the internal elastic lamina, with intima cell hyperplasia resulting in lumen stenosis. The hyperplastic vascular walls were positive for IgG and IgM.</p><p><b>CONCLUSIONS</b>The etiology of Moyamoya disease may involve allergic angiitis. A possible mechanism is that proximal portions of the circle of Willis first develop chronic stenosis or occlusion, leading to compensatory small vessel proliferation, which perforates into the cerebral parenchyma.</p>

Dynamic alteration of gene expression of the platelet-derived growth factor on SAH with late responseive cerebral vasospasm in rat / 中华老年医学杂志

Objective To investigate the effect of platelet-derived growth factor(PDGF) in DCVS diseases after SAH. Methods Alteration of PDGF gene and mRNA expression in DCVS brain tissues after SAH by RT-PCR and immunohistochemistry. Results To compare with the control group, transcription level of PDGF mRNA up-regulated evidently 3 days after transfusion (P

Experimental study on etiology and pathogenesis of the small intracranial aneurysm in rabbit / 中华神经科杂志

Objective To invectigate the etiology and pathogenesis of intracranial aneurysm. Methods Four experimental animal models of intracranial aneurysm were established with normal horse serum injected into veins or the neck regions, or into both common carotid arteries which were narrowed, by using silk ligature or noradrenalin injection into the neck regions. Results Ten intracranial aneurysms were found in the experimental animal model.Conclusion The immunological response,arteryischemia and continuous vasospasm may be considered the direct pathogenic factors of the intracranial aneurysm.