RESUMO
Umbilical cord blood stem cells have gained notoriety in both clinical and research settings. The hallmark of hematopoietic stem cells is their potential regenerative therapy. This study aimed to assess the yield of umbilical cord blood CD34[+] cells as regard different obstetric and neonatal factors
Materials and Methods: Twenty umbilical cord blood samples from different clinical groups of newborns were included. Our research has concluded separation of momnonuclear blood cells umbilical cord blood, assay of viability and cell survival, magnetic labeling, enrichment and evaluation of CD34 cells by FACScan flow cytometer
Results and conclusion: our results for hematopoietic stem cells concentrations suggest that the most valuable UCB units for banking and thus transplantation come from full term, bigger babies who are born to younger mothers with few previous pregnancies. Further studying of these obstetric factors and other factors should be continued for confirmation or revealing more facts as regard our locality