RESUMO
The current study was aimed at screening the Bunium bulbocastanum for its antibacterial, antifungal, phytotoxic and haemagglutination activities. The crude methanolic extract and n-hexane fraction showed significant [89%] and good activity [61%] against Staphylococcus aureus while the CHCl3 fraction was moderately active against S.aureus [53%]. Moderate activity was shown by the EtOAc fraction against B. subtilis [44%]. This fraction was inactive against P.aerogenosa and S.aureus. The aqueous fraction showed significant activity against B. subtilis [85%], moderate against S.aureus [34%] and E. coli [33%] and low activity against P.aerogenosa [29%]. Our results for antifungal assay indicated that all the test samples were inactive against all the test fungi. The phytotoxic activity of the plant at 1000 and 100 microg/ml was: crude methanolic extract [53.33 and 46.66%], n-hexane [46.66 and 26.66%], CHCl[3] [20 and 6.66%], EtOAc [46.66 and 26.66%] and aqueous [40 and 33.33%]. All the test samples [crude methanolic extract and fractions] of B. bulbocastanum were unable to agglutinate RBCs of the human blood indicating that this species lack phytolectins