In vitro Synergistic Action of Honey and Essential Oils against Two Species of Aspergillus: A Preliminary Study.

In order to evaluate the synergistic action of essential oils (EO) on the antifungal activity of honey, a comparative method of adding honey with and without EO to culture media was used. One variety of honey and five EO types were used to determine the minimum inhibitory concentration (MIC) against two clinical isolates of microfungi; namely Aspergillus niger and Aspergillus flavus. In a second step, lower concentrations of honey than the MIC were mixed with a set of sub-MIC of EO and then added to media to determine the minimum synergistic inhibitory concentration (MSIC). The MIC of honey without EO was 47% (v ⁄ v) against A. niger and 50% (v ⁄ v) against A. flavus. The MIC of EO varied strongly from one variety to another one with that of Eugenia caryophylata being the most effective against the tested Aspergillus species. When the EO was mixed with honey, a MIC drop was observed with each variety of EO. Isobolographic representation shows a synergistic action between honey and all EO types against the tested fungi. Further research studies are needed to elucidate and optimize the effective combination of these natural products in clinical practice.

Prevalence of rotavirus (GARV) and coronavirus (BCoV) associated with neonatal diarrhea in calves in western Algeria

<p><b>OBJECTIVE</b>To study the prevalence of bovine group A rotavirus (GARV) and bovine coronavirus (BCoV) in diarrheic feces from calves and the sensitive's parameters such as age group and sex.</p><p><b>METHODS</b>Feces samples from 82 diarrheic dairy calves from farms around Tiaret (Western Algeria) were collected. These samples were tested by ELISA assay.</p><p><b>RESULTS</b>The results showed that the prevalence of rotavirus and coronavirus infection are 14.63% (12.2% alone and 2.43% associated with bovine coronavirus) and 20.73% (18.3% alone and 2.43% associated with GARV), respectively.</p><p><b>CONCLUSIONS</b>The present study demonstrates that the both BCoV and GARV are involved in the neonatal calves' diarrhea, where the frequency of BCoV is clearly higher than that of GARV.</p>

Synergistic Effect of Monofloral Honeys and Essential Oils against Pseudomonas aeruginosa.

Aims: The emergence of pathogenic bacterial strains with resistance to commonly used antibiotics has necessitated a search for novel types of antibacterial agents. The main objective of this study was to evaluate the synergistic action between honeys and the essential oils (EOs) against Pseudomonas aeruginosa (ATCC 27853). Study Design and Methodology: In the first step, the minimum inhibitory concentration (MIC) of each honeys and Eos were determined. In the second step, lower concentrations of honey than the MIC were mixed with a set of sub-MIC of EOs and then added to media to determine the minimum synergistic inhibitory concentration (MSIC). Place of Study: Laboratory Research onof Local Animal Products, Ibn-Khaldoun University, Tiaret, Algeria. Results: The results indicated that the essential oils and all varieties of honey were effective against P. aeruginosa. The effectiveness was correlated to the botanical origin of honey and EOs. Wild carrot honey and Origanum vulgaris EOs were the most effective against the tested bacteria with a MIC of 8% and 2% respectively. Adding EOs to honey decreases the MIC values and the isobolographic representation shows a synergistic action between the EOs and all varieties of honey. Conclusion: The current prevalence of antibacterial resistant species has led to a re-evaluation of the therapeutic use of ancient remedies, including honey and EOs, which may receive renewed recognition as wound and burn healers

Antibacterial activity of various honey types of Algeria against Staphylococcus aureus and Streptococcus pyogenes

OBJECTIVE@#To assess the in vitro antibacterial activity of honey from different geographical location on Gram negative organisms.@*METHODS@#Different concentrations (Undiluted honey, 10 %, 30%, 50% and 70% wt/vol) of honey were studied in vitro using Staphylococcus aureus (S. aureus) and Streptococcus pyogenes (S. pyogenes), briefly, two-fold dilutions of honey solutions were tested to determine the minimum inhibitory concentration (MIC) against each type of microorganism, followed by more assays within a narrower dilution range to obtain more precise MIC values. MICs were determined by both visual inspection and spectrophotometric assay at 620 nm. These honey samples were compared with standard antibiotics like ampicillin, penicillin G, amoxicillin, gentamycin, tobramycin, erythromycin and chloramphenicol was determined by the disc diffusion method.@*RESULTS@#The diameter of zone of the inhibition (ZDI) of honey has various concentrations tested for the isolates ranged 0-46 mm for S. aureus, 0-44 mm for S. pyogenes. While the MIC (%) ranged 12%-95%, 25%-73% respectively.@*CONCLUSIONS@#Algeria honey, in-vitro, possess antibacterial activity.