RESUMO
Cinnamic acid derivative compound was investigated for the anti-cancer inhibitory activity. To be able to obtain compounds that have bioactivity as above, it is needed to study quantitative structure-activity relationship (QSAR) which is the process by which the chemical structure is quantitatively correlated with biological activity/chemical reactivity. Chemical methods used in synthesizing the chemical of methyl trans-cinnamate derivatives are tailored to match their targeted bioactivities. Here, we investigated the anti-cancer inhibitor compound with the method amidation of cinnamic acid derivative compounds. In this reaction we use two steps to get the target product. Firstly, we hydrolize of methyl trans-cinnamate to cinnamic acid. That reaction has a yield 85.5 % and secondly, we amidate of cinnamic acid to metil 2-cinnamamido-3-hydroxy propanoate has a yield of 51.5%. The compound of metil 2-cinnamamido-3-hydroxy propanoate showed against P388 leukemia cells inhibitory activity with IC50 = 10.78 μg/mL.
RESUMO
A sunscreen active compound has been isolated from the ethanol extract of Garcinia mangostana Linn (Clusiaceae). The ethanol extract was fractionated by liquid liquid extraction with n-hexane, methylene chloride and ethyl acetate, followed by further fractionation and purification using column chromatography on silica gel and gradient elution with combinations of n-hexane and ethyl acetate. Isolation and fractionation was guided by sunscreen activity assay. One of the active compounds was identified as αmangostin based on LC-MS and NMR Spectroscopy. The SPF value of the isolated α –mangostin at 50 and 100 ppm were 21.76 and 37.18, respectively and were much higher than the SPF values of the fractions obtained by liquid-liquid extraction.