The expression and clinical significance of hsa-miR-144 in esophageal squamous cell carcinoma / 天津医药

Objective To investigate the expression of hsa-miR-144 in esophageal squamous cell carcinoma, and its relationship with clinicopathological features and prognosis. Methods Reverse transcriptase polymerase chain reaction (RT-PCR) method was used to detect the hsa-miR-144 in 46 cases of esophageal squamous cell carcinoma and adjacent normal tissue. The expression of hsa-miR-144 in esophageal squamous cell carcinoma and its difference in the clinicopatho?logical characteristics including gender, age, and tumor size were investigated. The relationship between the expression of hsa-miR-144 and prognosis of patients with esophageal squamous cell carcinoma was analyzed. Kaplan-Meier method and Log-rank test were used to analyse the differences in survival rates in different pathological characteristics. Results The ex?pression level of hsa-miR-144 was lower in esophageal squamous cell carcinoma 0.97(0.22-24.48)×10-6 than that of adjacent normal tissue 8.60(0.09-258.20)×10-6, the difference was statistically significant (Z=2.221, P0.05). There was no correlation between the expression of hsa-miR-144 and prognosis in patients with esophageal squamous cell carcino?ma (rs=0.031, P=0.839). In the survival rate, there was no statistic significance between high expressive of hsa-miR-144 group and low expressive group (P=0.828). The survival rate was lower in patients with lymph node metastasis than that of pa?tients without lymph node metastasis. The survival rates were lower in patients with relatively deep invasion and higher patho?logic stage (P<0.05). Conclusion The expression of hsa-miR-144 is down regulated in esophageal squamous cell carcino?ma, and which is associated with lymph node metastasis and pathological staging of esophageal carcinoma. It shows that hsa-miR-144 may serve as an anti-oncogene in the occurrence and development of esophageal squamous cell carcinoma.

The changes and clinical significance of heart fatty acid-binding protein concentrations in children patients after open heart surgery / 中国医师进修杂志

Objective To observe the changes and clinical significance of plasma heart fatty acid-binding protein(H-FABP)in children after open heart surgery. Methods Forty patients with congenital heart disease(CHD) who had undergone cardiac operation with cardiopulmonary bypass(CPB) were selected in this study,and were randomly divided into two groups (each 20 cases): cold crystalloid cardioplegia perfusion group (Ⅰ group) and cold blood cardioplegia perfusion group(Ⅱ group). Blood samples were taken to check the plasma H-FABP concentration before CPB and different time after CPB. Plasma H-FABP concentration was estimated by enzyme-linked immunosorbent assay (ELISA). Results The difference of plasma concentration of H-FABP before operation was not obvious between Ⅰ group and Ⅱ group. The plas-ma concentration of H-FABP intraoperation and different time after operation was higher than that before anesthesia induction obviously in two groups, and Ⅰ group rose more significantly [(50.13±3.98) μg/L vs (39.27±4.22) μg/L, P<0.01]. Conclusions H-FABP concentration after operation is higher than that before operation significantly. H-FABP can be used to judge myocardial damage earlier as a sensitive index, because plasma concentration of H-FABP increases significantly after open heart surgery, and its peak emerges early. Compared with cold crystalloid cardioplegia,the myocardial protection of cold blood cardioplegia is better.

Expression of angiopoietin-2 and its relationship with microvessel density in sqnamous carcinoma of esophagus / 中国医师进修杂志

Objective To examine the expression of angiopoietin-2(Ang-2) in squamous carcinoma of esophagus,and discuss the relationship between Ang-2 and microvessel density(MVD)and clinical pathological features of squamous carcinoma of esophagus.Method The expression of Ang-2 and CD34 in 80 squamous careinoma of esophagus and 25 normal esophagus were detected by immunohistochemistry SABC technique,and the MVD of tissues signed by CD34 was counted.Results The expression of Ang-2 in squamous carcinoma of esophagus was,higher than that in normal esophagus (P<0.05).The expression of Ang-2 in the stage O-I and II squamous carcinoma of esophagus was lower than that in stage Ⅲ and lV(P<0.05).The expression of Ang-2 in squamous carcinoma of esophagus was correlated with differentiation of esophageal carcinoma and lymph node metastasis and infiltrative deepness.There was a remarkable posidve correlation between the expression of Ang-2 and MVD in squamous carcinoma of esophagus (r=0.603,P<0.05). Conclusion The expression of Ang-2 may be elated to the development of squamous carcinoma of esopha- gus,and may promote the development of squamous carcinoma of esophagus by promoting angiogenesis in tumor.

Correlation and expression of PTEN and vascular endothelial growth factor in human esophageal carcino-ma / 中国综合临床

Objective To investigate the expression of PTEN and vascular endothelial growth factor (VEGF) in esophageal carcinoma and the relationship between their expression.Methods The expression of PTEN and VEGF were detected using immunohistochemical S-P method.Results Among 80 cases of esophageal carcino- ma,31 showed positive staining of PTEN (38.75%),while all 20 case of normal mucosa showed positiva staimng of PTEN.The expression level of PTEN in highly differentiated squamous carcinoma was higher than that low differ- entiated squamous carcinoma.Also,the expression of PTEN was significantly correlated with lymph node metastasis (r=0.61,P<0.01)and differentiation(r=0.57.P<0.05).In 80 cases of esophageal carcinoma,57(70.13%) were of positive staining of VEGF,while in 20 of normal esophageal mucosa,only 3 showed positive staining of VEGF.The expression of VEGF was markedly correlated with infiltrative deepness(r=0.49,P<0.05)and lymph node metastasis(r=0.55,P<0.05)and differentiation(r=0.48,P<0.05).Conclusion Combined detection of PTEN and VEGF maybe helpful to evaluate prognosis and infiltrative capability of esophageal carcinoma,with sig- nificant importance to the prediction of the prognosis of esophageal carcinoam.

Cellular Immunity and Clinical Research on the Patients with Esophageal Carcinoma Underwent Resection and Preventive Thoracic Duct Ligation / 中国医师杂志

0 05). Lymphatic deposit and edema were not observed in follow-up cases. Conclusions Thoracic duct ligation could prevent the patients with esophageal carcinoma underwent resection from postoperative chylothorax. It can neither damage the cellular immunity nor lymphatic circulation.

EFFECT OF TRANSGENIC MT-GENE MUSHMOOM (PLEUROTUS OSTREATUS) ON ANTI-OXIDATION ABILITY OF IRRADIATED MICE / 营养学报

Objective: To explore the effect of transgenic metallothionein (MT) mushroom on anti-radiation ability of mice. Method: Dried power of transgenic MT mushroom was mixed into the feed of ICR mice. After being fed for 23 d, they were irradiated by 5.0Gy 60Co-?rays. Then their anti-radiation ability was determined after being fed 7 d again. Results: Transgenic MT mushroom can evidently alleviate the injury of platelet and spleen induced by radiation, and apparently increase SOD activity and reduce MDA content in irradiated mice’s serum. Conclusion: Transgenic MT mushroom can improve anti-oxidation ability of irradiated mice.