The effect of EGF on the cell-cycle of estrogen-negative breast cancer cell line MDA-MB-435S / 中国普通外科杂志

Objective To investigate the mechanism by which epidermal growth factor (EGF) accelerates the growth of estrogen-negative breast cancer cell line MDA-MB-435S. Methods Under the effect of EGF cyclin D_1 was measured by Western blot and cell-cycle was assayed by FCM. Results EGF enhanced cyclin D_1 expression. Protein kinase C (PKC) inhibitor Go6976 blocked cyclin D_1 over-expression caused by EGF. In EGF group G_0/G_1 was 69.36%, phase S was 22.77% and PI was 0.31. Differences were all significant compared with control (P