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Objective@#To investigate relationship between online partner seeking sexual behavior and sensation seeking risk behaviors among college students, and to provide suggestions on college sex education.@*Methods@#Using convenience sampling, a self administered questionnair survey was administered among 1 923 students in one college in Chongqing between September and October in 2021, regarding online partner seeking sexual behavior,sensation seeking and risk behaviors.@*Results@#About 26.7% of participants reported having sexual behavior, and 12.3% students having online partner seeking sexual behavior. There were no significant sex differences in online partner seeking sexual behavior ( χ 2=1.25, P >0.05); partner seeking sexual behavior varied by grade, gambling,alcohol drinking and internet addiction tendency( χ 2=9.40, 3.29, 19.63 and 3.66, P <0.05), which was higer among college students in grade one(9.1%), no gambling habit(12.0%), drinking habit(8.4%), and having no inclination to Internet addiction (11.8%) compared with those of grade 2 and 3(14.4%, 13.1%), gambling habit(19.7%), no drinking habit( 15.1 %) and having inclination to Internet addiction (16.6%). The results of multivariate Logistic regression showed that alcohol drinking ( OR =0.45，95% CI =0.33-0.62) was negatively associated with online partner seeking sexual behavior, sensation seeking ( OR =1.08，95% CI =1.03-1.13) and gambling ( OR =2.15，95% CI =1.19-3.88) were positively associated with online partner seeking sexual behavior of college students( P <0.05).@*Conclusion@#Colleges should pay attention to the occurrence of online partner seeking sexual behavior, provide guidance for recognition of adverse consequences of sensation seeking behavior, and to avoid the clustering of online partner seeking sexual behavior with gambling behaviors.
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Objective@#To investigate the relationship between breakfast eating frequency and health-risk behavior of high school students in Chongqing, and provide a reference for conducting students to get a good habit of eating breakfast everyday.@*Methods@#A total of 3 283 high school students in Chongqing were selected to participate in a questionnarie by using convenience sampling method. Logistic regression analysis were condueted to analyze the influences made by the unhealty eating behaviors on eating frequency of breakfast.@*Results@#There were 2 064(69.2%) students eating breakfast everyday, 1 219(37.1%) students having no breakfast or eating breakfast sometimes. There were significant differences between different gender, grade, native place, drinking energy drink, smoking, drinking alcohol, gambling, internet addiction students rate of unhealthy breakfast eating frequency(χ2=6.80, 53.97, 8.10, 23.79, 7.89, 16.31, 3.93, 17.96, P<0.05), the males (36.1%) was beyond the females(33.3%), the energy drink drinkers(41.2%) was beyond the non-drinkers(31.7%), the smokers (40.9%) was beyond the non-smokers(34.4%), the alcohol drinkers(40.3%) was beyond the non-drinkers(33.3%), the gamblers(39.7%) was beyond the non-gamblers(34.3%), those having internet addictions (40.0%) was beyond those having no addictions(33.0%). Drinking energy drink(OR=1.32, 95%CI=1.12-1.55), drinking alcohol(OR=1.24, 95%CI=1.02-1.51), internet addiction(OR=1.42, 95%CI=1.19-1.68) were positively correlated with unhealthy breakfast eating frequency of high school students(P<0.05).@*Conclusion@#Social, families and schools should strengthen the guidance of high school students to develop the habit of eating breakfast every day, and advoid the clustering of skipping breakfast and health-risk behaviors.
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Objective To investigate the role and mechanism of RSPO1 in osteoblasts differentiation.Methods The xCELLigence system was used to study the toxicity and role of RSPO1 on the C2C12 cells proliferation.Alkaline phosphatase (ALP) activity was measured by using a phosphatase detection kit.The expression of osteoprotegerin (OPG) was determined using enzyme-linked immunosorbent assay (ELISA).Wnt/β-catenin signaling was evaluated using the TOPflash T cell factor (TCF) luciferase reporter assay.Western blotting assay was performed to confirm the accumulation of β-catenin protein.T test was used for statistical analysis.Results RSPO1 had no effect on the C2C12 cells proliferation,and it produced no toxicity to C2C12 cells.RSPO1 alone resulted in a slight increase in the activity of ALP (2.85±0.08 vs 1.74±0.21,t=3.014,P＜0.05) and the expression of OPG (1.29±0.13 vs 1.00±0.21,t=3.348,P＜0.05),whereas the combination of RSPO1 and Wnt3A significantly amplified ALP activity (81.37±5.08 vs 1.74±0.21,t=31.31,P＜0.01) and OPG protein expression (5.26±0.60 vs 1.00±0.21,t=6.99,P＜0.01).RSPO1 synergized with Wnt3A to activate TCF activity and induce accumulation of β-catenin (3.28±0.18 vs 1.00±0.21,t=10.94,P＜0.05).However,RSPO1 alone had no effect on the TCF activity and β-catenin accumulation (1.25±0.08 vs 1.00±0.21,t=2.225,P＞0.05).Conclusion Our study has revealed that RSPO1 synergized with Wnt3A in activating Wnt/β-catenin signaling pathway to induce osteoblasts differentiation,which demonstrate the therapeutic potential of RSPO1 for RA.