Effect of Sishenwan， Baitouweng Tang， and Lianlitang on Ulcerative Colitis in Rats / 中国实验方剂学杂志

Objective:To explore the mechanism of Sishenwan， Baitouweng Tang， and Lianlitang in the treatment of ulcerative colitis （UC）， and compare their efficacies on UC in rats. Method:Ninety SD rats of SPF grade were randomly divided into blank group （distilled water， 2 mL·d^-1） and experimental group. The rats in the experimental groups were administered with 2，4，6-trinitrobenzene sulfonic acid （TNBS） by clysis to induce the UC model. Subsequently， the model rats were divided into a model group （distilled water， 2 mL·d^-1）， positive group ［sulfasalazine （SASP）， 0.4 g·kg^-1·d^-1］， Sishenwan group （1.76 g·kg^-1·d^-1）， a Baitouweng Tang group （1.40 g·kg^-1·d^-1）， and Lianlitang group （2.13 g·kg^-1·d^-1） according to the random number table. The rats in each group were dosed at 2 mL·d^-1 for 14 days. The pathological score for colonic mucosa was detected. Cytokines were detected by the cytokine chip. The enzyme-linked immunosorbent assay （ELISA） was used to detect the free triiodothyronine （FT₃）， glucagon-like peptide-1 （GLP-1）， and corticosterone （CORT） in plasma， and neurotensin （NT）， substance P （SP）， vasoactive intestinal peptide （VIP）， and somatostatin （SST） in colon tissues. Result:Compared with the normal group， the model group showed increased colon mass-length ratio and pathological score for colonic mucosa （<italic>P</italic><0.01）， infiltration of massive lymphocytes， disordered or absent intestinal villi， elevated levels of cytokine-induced neutrophil chemoattractant-1/2<italic>α</italic>/<italic>β</italic>/3 （CINC-1/2<italic>α</italic>/<italic>β</italic>/3）， interleukin-1<italic>α</italic> （IL-1<italic>α</italic>）， interferon-inducible protein-10 （IP-10） and other factors in colon tissues （<italic>P</italic><0.05）， dwindled CORT and GLP-1 levels in plasma （<italic>P</italic><0.05）， and increased SP content in colon tissues （<italic>P</italic><0.05）. Compared with the results in the model group， the mucosal injury in the colon of rats in each drug group was relieved. The levels of IL-1<italic>α</italic>， IP-10， lipopolysaccharide-inducible CXC chemokine （LIX）， and L-selectin of rats in the Lianlitang group and Sishenwan group were reduced （<italic>P</italic><0.05）， and the CINC-3 and IL-17 levels were diminished in the Baitouweng Tang group （<italic>P</italic><0.05）. The levels of CINC-1/3， IL-1<italic>α</italic>， and IP-10 were reduced in the SASP group （<italic>P</italic><0.05）. The plasma FT₃ was up-regulated in the Lianlitang group， and the plasma GLP-1 levels were elevated in the three Chinese medicine groups （<italic>P</italic><0.05）. The VIP content in colon tissues of the Sishenwan group and Baitouweng Tang group was down-regulated （<italic>P</italic><0.05）， and the SST content in colon tissues of the SASP group was significantly up-regulated （<italic>P</italic><0.01）. Conclusion:The intervention of Lianlitang and Sishenwan on UC was significant， and the underlying mechanism of action might be related to inflammation inhibition and immune balance by regulating the cytokine network. The efficacy of Lianlitang was predominant， followed by Sishenwan and Baitouweng Tang.

Effects of testosterone and cortisol in sports fatigued organism by Chinese material medica of shixiang yaofa / 中国中药杂志

<p><b>OBJECTIVE</b>To study the endogenous hormone of testosterone and cortisol that secretes volume and rhythm in sports fatigued human bodies and animals. To determine secretory volume and rhythm in sports fatigued human bodies and animals when Shixiang yaofa's drug are used.</p><p><b>METHOD</b>Radio-immunity method was used to determine secretory volume and rhythm in sports fatigued human bodies and animals and to analyze secretory volume and rhythm. According to the secretory volume and rhythm of testosterone and cortisol, the Shixiang Yaofa's drugs were used. Doses: wenyang jihuo bead 10 g/sack 2 sack, ziyin xiuyang capsule 0.5 g/pill 8 pill pd in human bodies for 28 days. Wenyang jihuo bead 10 g x kg(-1) of crude drug, ziyin xiuyang capsule 4 g x kg(-1) of crude drug pd for hight doses in animals. Other groups for low doses 5 g x kg(-1) and 2 g x kg(-1) of crude drug pd for 15 days. The blood samples were collected for determination.</p><p><b>RESULT</b>(1) In human bodies the peak value of testosterone was appeared in 8:00 and valley value was appeared in 18:00, ranges: 176.93-281.73 x 10(-5) mg x L(-1). The peak value of cortisol was appeared in 8:00 and valley value was appeared in 22:00, ranges: 1.31-16.13 x 10(-3) mg x L(-1). In the same condition, the mouse peak value of testosterone was appeared in 20:00 and valley value was appeared in 0:00, ranges: (0.56 x 10(-5) - 124.0 x 10(-5)) mg x L(-1). The rhythm in animals was compatible with human bodies, howerer, the peak value was delayed for 12 hours. (2) The testosterone was step up and the cortisol was cut down in sports fatigued human bodies and animals when shixiang yaofa's drug were used (P < 0.05, P < 0.01).</p><p><b>CONCLUSION</b>The secretion of testosterone and cortisol in sports fatigued human bodies and animals are existed conclusive biologic rhythm. The secretory volume can be available accommodation by shixiang yaofa's drugs.</p>

Effect of aniracetam on learning and memory dysfunction in mice with cerebral ischemia/reperfusion injury / 中国康复理论与实践

@#ObjectiveTo investigate the effects of Aniracetam on learning and memory dysfunction in mice with cerebral ischemia/reperfusion injury. Methods48 mice were randomly divided into control group, sham operated group, model group and Aniracetam group, with 12 mice each. Mice model of cerebral ischemia/reperfusion injury was established by bilateral common carotid artery blocking. The learning and memory function was tested by Morris water maze.ResultsThe latent period in the model group was longer than that in the normal group and the sham operated group (P<0.05). Aniracetam could shorten the latent period significantly as compared with those in the model group (P<0.05). ConclusionAniracetam has effects in improving learning and memory function in cerebral ischemia/reperfusion injured mice.

Effect of Erigeron breviscapus Hand-Mazz on learning and memory dysfunction in mice with cerebral ischemia/reperfusion injury / 中国康复理论与实践

@#Objective To investigate the effects of Erigeron breviscapus Hand Mazz(EBH) on learning and memory dysfunction in mice with cerebral ischemia/reperfusion injury.Methods 40 mice were randomly divided into control group, sham operated group, model group and EBH group, with 10 mice each. Mice model of cerebral ischemia/reperfusion injury was established by bilateral common carotid artery blocking. 7 days before and after operation, the mice in EBH group were injected I.p.with EBH injection 0.15 ml/d, while those in other groups were injected I.p. with saline 0.15 ml/d. The learning and memory function was tested by Morris water maze.Results The latent period in the model group was longer than that in the normal group and the sham operated group (P<0.05). EBH could shorten the latent period significantly as compared with those in the model group (P<0.05).Conclusion EBH can improve learning and memory function in cerebral ischemia/reperfusion injured mice.