RESUMO
The cord blood is a souce of hematopoietic stem cells. This have encouraged the creation of many cord blood banks around the world. By this prospective study during 2 years, we tryed to show the advantages of such sources of progenitors, and to evaluate our techniques of collection, freezing, biological and microbiological survey. For this aim, we tested 100 specimens of cord blood before and after defrosting. Our selection criteria were a volume >/= 60 ml, a CD 34+ cells count >/= 2. 10[6], bacteriological and serological negativity. This 100 specimens have an average volume of 95,22 ml, an average nuclear cells count of 10,95.10[6] and an average CD 34+ cells count of 2,96. 10[6]. The average cells viability after defrosting is 96,69%, and the average efficiency rate of 92,11%. The specimens selected according to our selection criterias were of 39%. Our results confirm the feasibility of a cord blood bank in the "Centre National de Transfusion Sanguine" in Tunis
Assuntos
Humanos , Feminino , Bancos de Sangue , Transplante de Células-Tronco de Sangue do Cordão Umbilical , Células-Tronco Hematopoéticas , Estudos ProspectivosRESUMO
So, the epidemiologic study done for 8 years at CMNR Tunis has shown that the incidence is 1,15%o births [between 0,52 and 1,66%o per year]. This anomaly is more frequent in Female sex [sex linked = 0,59]. The antenatal echographic diagnosis is efficient in 90,5%. This anomaly is more shown at low socio-economic group. The improvement of the socio-economic level and specially the daily uptake of acid folic in periconceptionnel time decrease the frequency of this congenital malformation
Assuntos
Humanos , Masculino , Feminino , Feto/patologia , Feto/anormalidades , Ultrassonografia Pré-Natal , Ácido FólicoRESUMO
Letal spina bifida continue to be frequent in Tunisia; we report 88 cases of letal spina bifida 1,05 per thousand births. This pathology was more frequent with women. The up letal spina bifida situated is predominant with female and the dow spina bifida situated is frequent with male. We have noted an association with anencephalia [46 cases] and hydrocephaly [21 cases]. Prevention is based on obstetric health care and hygieno dietetic advices to avoid alimentary deficit