RESUMO
BACKGROUND: At present, there are many types of bone defect repair scaffolds, but a single type of material is difficult to meet the requirements of bone tissue engineering scaffold materials. Several suitable materials can be combined into a composite material by appropriate methods, taking into account the advantages and disadvantages of various materials. It is the focus of scholars in recent years. OBJECTIVE: To construct nano-hydroxyapatite/chitosan/polycaprolactone composite scaffolds and analyze characterization of composite scaffolds. METHODS: Nano-hydroxyapatite/chitosan/polycaprolactone porous ternary composite scaffold material was prepared by 3D printing and molding technology. The characterization of scaffold material was studied from X-ray diffraction analysis, stent water absorption rate, stent compressive strength, stent degradation performance in vitro, stent aperture analysis, scanning electron microscope analysis and other dimensions. RESULTS AND CONCLUSION: (1) X-ray diffraction analysis showed that the crystal-shaped peak map of nano-hydroxyapatite/chitosan/ polycaprolactone scaffold materials was similar to the hydroxyapatite powder diffraction standard card, suggesting that the scaffold materials were integrated with each other through physical interaction, and did not affect the biological function of hydroxyapatite. (2) The average water absorption rate of the scaffold was 18.28%, and the hydrophilicity was good. The maximum pressure that the scaffold could withstand was 1 415 N, and the degradation rate was similar to the osteogenic rate. (3) Under a microscope, a ternary scaffold material with an aperture of 250 µm was successfully produced. The pore size was uniform and distributed regularly. (4) Scanning electron microscope demonstrated that the fibers composed of chitosan and polycaprolactone were arranged orderly and grid like, hydroxyapatite was distributed uniformly on the fiber surface in granular form, and the ternary composite material presented uniform and loose microporous structure. (5) Nano-hydroxyapatite/chitosan/polycaprolactone ternary composite scaffold material can be successfully prepared through 3D printing and molding technology, which has moderate compressive strength, certain porosity, appropriate degradation rate and water absorption rate, and can lay a foundation for repairing bone defects.
RESUMO
BACKGROUND:The physical properties and chemical composition of teeth are very similar with human bone tissue, and there are a larger proportion of inorganic components. Therefore, tooth can be considered as a potential repair material for autologous or alogeneic bone defects. OBJECTIVE:To prepare polyetheretherketone/odontogenic biphasic bioceramic composite, and to test its mechanical properties. METHODS: The humanin vitro teeth of clinical waste were colected. The organic components were removed after a preliminary calcination. Another calcination was conducted after soaking in diammonium phosphate solution for 24 hours to prepare the biphasic ceramics with the main components of hydroxyapatite and β-tricalcium phosphate. The biphasic ceramics were ground and sieved using 200-mesh sieve folowed by impregnation in organic foam to prepare polyetheretherketone/odontogenic biphasic bioceramics. Phase analysis, scanning electron microscopy, elemental analysis, porosity, compressive strength and bond strength test were conducted. RESULTS AND CONCLUSION:Polyetheretherketone/odontogenic biphasic bioceramics presented porous network structure and interconnected holes, with the aperture of 100-800 μm, porosity of 73.65%, compressive strength of (165.260±11.703) N, bond strength of (14.63±6.21) MPa. P element content accounted for 19.8%, and Ca element content accounted for 40.5%. The main phases were β- tricalcium phosphate and hydroxyapatite. These results demonstrate that polyetheretherketone/odontogenic biphasic bioceramics have good mechanical properties.