Involvement of COX-2 in antagonism to heme oxygenase-1 on cardioprotection from anoxia/reoxygenation induced injury in rats / 基础医学与临床

Objective To investigate the role of HO-1 in the protection of rat heart from anoxia/reoxygenation induced injury and its underlying mechanism.Methods LVEDP,LVDP and dp/dtmax were analyzed by the Langendorff method in isolated rat heart.Lactate dehydrogenase(LDH), infarct area,COHb and 6-keto-PGF1? were further determined in the experiment.Results After intraperitoneal injection of HO-1 inducer hemin,CO concentration in rat blood enhanced(P

Role of heme oxygenase-1 in cardioprotection against anoxia/reoxygenation induced injury and its mechanisms / 中国病理生理杂志

AIM: To investigate the role of HO-1 in protection of rat hearts against anoxia/reoxygenation-induced injury and its underlying mechanism. METHODS: Cardiac contractility, lactate dehydrogenase (LDH) and infarct area were analyzed by the Langendorff method in isolated rat hearts. RESULTS: After intraperitoneal injection of HO-1 inducer hemin, CO concentration in rat blood enhanced (P

Vasodilatation induced by Jumi extraction and its mechanisms / 中国病理生理杂志

AIM: The objectives of the present study were to examine the effect of Jumi(JM) extraction on relaxation of isolated rat aortic rings,and to elucidate its mechanisms.METHODS: The thoracic aortic rings with and without endothelium of male Sprague-Dawley rats were mounted on a bath system.Vasodilatation of aortic rings preconstricted with 10-6 mol/L of phenylephrine(PE) was measured.RESULTS: JM extraction(0.5-8 g/L) caused a concentration-dependent relaxation in aortic rings.The extent of relaxation was larger in endothelium-intact aortic rings than that in endothelium-denuded aortic rings.Both L-NAME [a nitric oxide synthase(NOS) inhibitor] and high potassium(20 mmol/L KCl) partly abolished the relaxation action of JM extraction in endothelium-intact aortic rings.Pretreatment with L-NAME also inhibited the relaxation response to JM extraction in endothelium-denuded aortic rings.After incubation with JM extraction,NOS activities enhanced both in endothelium-intact and endothelium-denuded aortic rings.CONCLUSION: JM extraction causes relaxation of aortic rings through endothelium-dependent and independent pathways.The mechanisms might be involved in NOS and endothelium-derived hyperpolarizing factor.