Physicochemical characterization and microbiological analysis of Oligosarcus robustus raw fish meat and development a fish hamburger / Caracterización físico-química y análisis microbiológico de la carne cruda de pescado Oligosarcus robustus y desarrollo de una hamburguesa de pescado

ABSTRACT This study aimed to evaluate the microbiological, physicochemical and fatty acid profile of the of "Tambica" (Oligosarcus robustus) raw fish meat and develop and characterize a fish burger. The fish burger was subjected to a sensory analysis. The raw fish meat showed a high content of moisture (82.3%) and minerals (3.1%), a low content of fat (1.5%) and a considerable protein content (13.1%). The raw fish showed a high count of positive coagulase Staphylococcus. Tambica lipid fraction was composed of 41.9% unsaturated and 58.2% saturated fatty acids. Palmitic acid and oleic acid were the major fatty acids in the raw meat fish. The fish burger was well accepted by sensory analysis.

RESUMEN Este estudio tuvo como objetivo evaluar el perfil microbiológico, fisicoquímico y de ácidos grasos de la carne de pescado cruda "Tambica" (Oligosarcus robustus); y desarrollar y caracterizar una hamburguesa de pescado. La hamburguesa de pescado fue sometida a análisis sensorial. La carne de pescado cruda mostró un alto contenido de humedad (82,3%) y minerales (3,1%), bajo contenido de grasa (1,5%) y un contenido de proteína considerable (13,1%). El pescado crudo mostró un recuento alto de Staphylococcus coagulasa positivo. La fracción lipídica de Tambica estaba compuesta por un 41,9% de ácidos grasos insaturados y 58,2% de ácidos grasos saturados. El ácido palmítico y el ácido oleico fueron los principales ácidos grasos en el pescado de carne cruda. La hamburguesa de pescado fue bien aceptada por el análisis sensorial.

Chemical characterization, antimicrobial and antioxidant activity of sugar-apple (Annona squamosa L. ) pulp extract / Caracterización química, actividad antimicrobiana y antioxidante del extracto de pulpa de manzana de azúcar (Annona squamosa L. )

Synthetic preservatives are widely present in processed foods, but most of them have carcinogenic potential, requiring the development of new natural alternatives such as fruit extracts, for microbial control. The objective of the study was to evaluate the chemical characterization, antioxidant, and antimicrobial activity of the sugar apple pulp (Annona squamosa L.). Physicochemical characteristics were evaluated, an extract was prepared, and its antioxidant activity by DPPH method and antimicrobial by disk diffusion. Minimal inhibitory concentration and minimum bactericidal concentration against strains of Salmonella typhimurium, Escherichia coli, Listeria monocytogenes, and Staphylococcus aureus were evaluated. The physicochemical analysis revealed that sugar apple pulp had 75.0% moisture, 3.0% ash, 4.0% protein, 0.2% lipids, 3.3% fibers, and 14.5% carbohydrates. The antioxidant activity of the extract by the DPPH method was 20.6%. The pulp extract from the sugar apple had inhibition zone for Staphylococcus aureus, satisfactory inhibitory effect against Staphylococcus aureus, Escherichia coli, Listeria monocytogenes, and Salmonella Typhimurium, but did not present a bactericidal effect. Sugar apple pulp presents adequate levels of nutrients and potential for food application due to its microbiological activity and antioxidant properties.

Los conservantes sintéticos están ampliamente presentes en los alimentos procesados, pero la mayoría tienen potencial carcinogénico, lo que requiere el desarrollo de nuevas alternativas naturales para el control microbiano, como los extractos de frutas. El objetivo del estudio fue evaluar la caracterización química, la actividad antioxidante y antimicrobiana de la pulpa de manzana de azúcar (Annona squamosa L.). Se evaluaron las características fisicoquímicas, y se evaluó su actividad antioxidante mediante el método DPPH y antimicrobiano por difusión en disco, concentración inhibitoria mínima y concentración bactericida mínima contra cepas de Salmonella Typhimurium, Escherichia coli, Listeria monocytogenes y Staphylococcus aureus. El análisis fisicoquímico reveló que la pulpa de manzana de azúcar tiene 75.0% de humedad, 3.0% de cenizas, 4.0% de proteínas, 0.2% de lípidos, 3.3% de fibras y 14.5% de carbohidratos. La actividad antioxidante del extracto por el método DPPH fue del 20.6%. El extracto de pulpa de la manzana de azúcar tenía zona de inhibición para Staphylococcus aureus, efecto inhibidor satisfactorio contra Staphylococcus aureus, Escherichia coli, Listeria monocytogenes y Salmonella Typhimurium, pero no presenta efecto bactericida. La pulpa de manzana de azúcar presenta niveles adecuados de nutrientes y potencial para la aplicación de alimentos debido a su actividad microbiológica y propiedades antioxidantes.

Condições higienicossanitárias dos serviços de alimentos e bebidas em hotéis do município de Pelotas ­ RS / Hygienic sanitary conditions of food and beverage services in hotels in the municipality of Pelotas ­ RS

Atualmente observa-se expansão do mercado hoteleiro e, consequentemente, cresce a preocupação com o fornecimento de refeições seguras, distribuídas neste serviço. O objetivo deste estudo foi realizar uma avaliação das condições microbiológicas do setor de alimentos e bebidas dos hotéis no município de Pelotas/RS e realizar um treinamento dos manipuladores, com base no conceito de boas práticas. Equipamentos e utensílios foram analisados quanto à enumeração de mesófilos aeróbios e os resultados discutidos conforme as sugestões da American Public Health Association (APHA). Para a análise das mãos dos manipuladores realizou-se a enumeração de estafilococos coagulase positiva e os resultados discutidos conforme organização Mundial da Saúde (OMS), Organização Panamericana de Saúde (OPAS) e Associação Brasileira de Estabelecimentos de Refeições Coletivas (ABERC). Participaram deste estudo quatro hotéis localizados na área central de Pelotas. Após o treinamento, foram verificadas reduções (p<0,05) nas contagens de mesófilos aeróbios na placa de corte e xícara do Hotel 2. De um modo geral, equipamentos e utensílios apresentaram contaminações dentro do limite sugerido pelas instituições, após o treinamento. Mãos de manipuladores não diferiram estatisticamente, porém apresentaram reduções nas contagens de estafilococos coagulase positiva, após o treinamento. Conclui- se que o treinamento em boas práticas ofertado aos manipuladores de alimentos contribuiu para implementação da qualidade dos produtos e serviços oferecidos. Entretanto, um treinamento apenas, não é o suficiente para assegurar a redução da carga microbiana de equipamentos, utensílios e mãos de manipuladores.

Sensibility and resistance profiles to antibiotics of pathogens isolated in a hospital unit of food and nutrition / Perfil de sensibilidade e resistência a antimicrobianos de patógenos isolados em uma unidade de alimentação e nutrição hospitalar

The objective of this study was to evaluate the sensibility and resistance profiles to antibiotics of pathogens isolated in a hospital unit of food and nutrition. It was evaluated as sample spots, with repetitions, environmental samples, utensils, equipment, manipulators' hands and usually diet that would be served to the hospitalized patients. In the evaluated samples, coagulase positive Staphylococcus (CPS) and Gram negative bacillus were enumerated, and also, it was possible to isolate Klebsiella spp and Escherichia coli. The antimicrobial of less efficiency for CPS was oxacillin and penicillin-G, and for Klebsiella spp ampicillin e cephalothin. It should be emphasized that it was found strains multiresistants of CPS, Klebsiella spp and E. coli. The hospital unit of food and nutrition was evaluated as microbiological inadequate in several points, and it was verified resistance profiles to antibiotics of pathogens isolated in this unit.

O objetivo deste estudo foi avaliar os perfis de sensibilidade e resistência a antibióticos de patógenos isolados em uma unidade de alimentação e nutrição hospitalar. Foram analisados como pontos de amostragem, com repetições, de ambientes, de utensílios, de equipamentos, de mãos de manipuladores e de dieta normal a qual seria servida a pacientes hospitalizados. Nas amostras analisadas, Estafilococos coagulase positiva (ECP) e bacilos Gram negativos no ágar Mac Conkey foram enumerados, e Klebsiella spp e Escherichia coli foram isoladas. Os antimicrobianos de menor eficiência para ECP foram oxacilina e penicilina-G e para Klebsiella spp ampicilina e cefalotina. Cabe ressaltar que foram encontradas cepas multirresistentes de ECP, Klebsiella spp e E. coli. A unidade de alimentação e nutrição hospitalar foi avaliada como inadequada do ponto de vista microbiológico em diversos pontos e verificou-se a presença de perfis de resistência a antibióticos de patógenos isolados nesta unidade.

Age at onset of caloric restriction and its effects on the redox profile of the rat hippocampus

BACKGROUND: The benefits of caloric restriction (CR) on the protection against age-related neurodegenerative diseases have been the subject of several studies. However, the effects of CR on the central nervous system are still poorl y understood since most studies were carried out in mature animals. The present study aimed to investigate whether the age at onset of CR could differently affect the redox status of the rat hippocampus. METHODS: Thirty-two male Wistar rats at 35 days old (35d;n= 16) and 65 days old (65d;n= 16) were fed ad libitum or subjected to 30 % CR (n= 8 group/age) for 12 weeks. At the end of the experiment, the rats were euthanized, blood was collected, and the hippocampus was dissected for measuring the redox status. RESULTS: CR in 35d and 65d rats induced a 16 and 21% reduction in body weight gain, respectively, compared to controls (p< 0.05). Urea, total cholesterol, triacylglycerol, HDL cholesterol, and LDL cholesterol concentrations were lower in CR 35d rats than in 35d controls (p< 0.05). No differences were detected between the CR groups and controls in the object recognition test (p> 0.05) and in superoxide dismutase activity, nitric oxide content, and lipid peroxidation levels(p> 0.05). However, glutathione peroxidase activity was higher (p< 0.0001) in 65d rats compared to that in 35d rats, and GSH content was higher (p< 0.05) in CR-fed rats compared to that in controls at both ages. CONCLUSIONS: In conclusion, CR increased GSH content when started at both ages but did not affect the activity of antioxidant enzymes and the level of ROS in the hippocampus. In addition, CR did not induce any detrimental effects on memory and nutritional status when started in both 35d and 65d rats

Coconut oil on biochemical and morphological parameters in rats submitted to normolipidic and hyperlipidic diets / Óleo de coco em parâmetros bioquímicos e morfológicos de ratos submetidos à dieta normolipídica ou hiperlipídica

ABSTRACT: This study aimed to evaluate the influence of replacing soybean oil with extra virgin coconut oil in normolipidic and hyperlipidic diets, on the lipid metabolism of Wistar rats. In the first stage of the experiment (30 days), 36 rats were divided into 2 groups and fed with a control or a hyperlipidic diet. Six animals from each group were then killed, and the remaining rats were redistributed into 4 new groups: 2 groups remained on the control and hyperlipidic diets, and in the diets of the other 2 groups, the soybean oil was replaced with coconut oil (30 days). At the end of the assay, the biological models were decapitated for blood collection and removal of organs and peritoneal fat. Although the diet intake differed among groups during both stages of the experiment, no differences were noted with regard to weight gain and peritoneal fat. Replacing soybean oil with coconut oil in the rat diet lowered triglyceride and low-density lipoprotein serum concentrations in both groups. Liver parameters, namely, total cholesterol and triacylglycerols, increased with the substitution of soybean oil by coconut oil in the normolipidic diet and decreased in the hyperlipidic diet. Thus, replacing soybean oil by coconut oil may improve serum and liver lipid levels in Wistar rats.

RESUMO: O estudo teve como objetivo avaliar a influência da substituição do óleo de soja por óleo de coco extravirgem em dietas normolipídicas e hiperlipídicas, sobre o metabolismo lipídico de ratos Wistar. Na primeira fase do experimento, 30 dias, 36 ratos foram distribuídos em dois grupos, receberam dieta controle e dieta hiperlipídica. Após eutanásia de seis animais por grupo, os ratos de ambos os grupos foram redistribuídos em quatro novos grupos, dois permaneceram com as dietas controle e hiperlipídica e nos outros dois houve substituição do óleo de soja por óleo de coco (30 dias). Ao final do ensaio, os modelos biológicos foram decapitados, para coleta de sangue, retirada de órgãos e gordura peritoneal. O consumo de dieta foi diferente entre os grupos durante as duas fases do experimento, porém sem diferença no ganho de peso e gordura peritoneal. A substituição, na dieta, do óleo de soja por óleo de coco diminuiu as concentrações séricas de triacilgliceróis e de lipoproteínas de baixa densidade, em ambos os grupos. Os parâmetros hepáticos colesterol total e triacilgliceróis aumentaram com a substituição do óleo de soja por óleo de coco em dieta normolipídica e diminuíram na dieta hiperlipídica. A substituição do óleo de soja por óleo de coco pode ter benefícios com relação aos lipídeos séricos e hepáticos em ratos Wistar.

Lipid profiles of rats fed with diets supplemented with vitamins niacin and pyridoxine

Hypercholesterolemia is a major risk factor for cardiovascular disease. Supplements containing the vitamins niacin (B3) and pyridoxine (B6) can promote the reduction of total cholesterol and an increase in HDL cholesterol. In this study, the effects of diets supplemented with niacin (B3) and pyridoxine (B6) on the hepatic and serum lipid profiles of Wistar rats were assessed. The diets were prepared with combinations of three concentrations of niacin (3, 4 and 5 g/kg) and pyridoxine (6, 12 and 18 mg/kg) and one with neither vitamin. The animals were divided into eleven experimental groups of six animals per group, and nine groups were fed on a standard diet with 7.5% fat and vitamin supplementation. Another group was fed with 7.5% fat without vitamin supplements. A control group received the standard diet (AIN-93M) without modifications (4% fat). The weight gain, food intake, serum and hepatic total cholesterol, serum cholesterol fractions (HDL, LDL, and VLDL), serum and hepatic triacylglycerols and hepatic and fecal lipid contents were measured after 30 days. The diet with the highest concentration of niacin and lowest concentration of pyridoxine had the lowest level of total hepatic cholesterol. Hepatic triacylglycerols were reduced by the highest concentration of niacin (5 g/kg), and this reduction was enhanced by increasing the pyridoxine concentration. The diets supplemented with niacin and pyridoxine reduced the levels of serum total cholesterol, LDL, VLDL, triacylglycerols and hepatic lipids. These effects on the lipid profile varied with the concentrations of the two vitamins and the interactions between them.

Arroz parboilizado efeito na glicemia de ratos Wistar

O amido é uma importante fonte energética podendo representar mais de 60% da ingestão calórica na dieta humana. A fração do amido, que é resistente à digestão enzimática, é denominada de amido resistente. Na parboilização de arroz ocorre a retrogradação do amido, com a formação do amido resistente tipo 3 (retrogradado), que apresenta efeitos benéficos à saúde, por atuar como prébiótico. Neste estudo, três amostras de arroz com alta, média e baixa amilose foram selecionadas, com o objetivo de avaliar o efeito de arroz convencional e parboilizado na glicemia de ratos Wistar. As amostras com alta e média amilose foram encharcadas durante 6h a 65°C e a amostra com baixa amilose durante 7h a 70°C, posteriormente foram autoclavadas em pressão 0,7 kgf.cm-2 durante 10 minutos. Utilizouse 6 ratos Wistar, machos, adultos por tratamento. Foram elaboradas 7 dietas experimentais, formuladas de acordo com AIN-93M, dieta controle e, dietas com substituição da fonte de carboidratos por arroz convencional e parboilizado. Determinou-se amido resistente nas dietas e a glicemia foi monitorada por leitura de glicofita, com sangue da parte distal da cauda do rato. Para a curva glicêmica as medidas de glicemia foram feitas em jejum, e ao longo de 90 minutos. Os resultados indicam que não foram encontradas diferenças significativas entre as dietas formuladas com arroz de alta, média e baixa amilose, parboilizado ou convencional na glicemia de jejum e pós-prandial de ratos Wistar.

The effect of parboiled rice on glycemia in Wistar rats. Starch is an important energy source and can represent more than 60% of the calories of the human diet. The starch fraction resistant to enzymatic digestion is called resistant starch. When rice is parboiled, the starch retrogrades with the formation of type 3- resistant starch (retrograded), which presents beneficial effects on the health, since it acts as a prebiotic. In the present study three types of rice were selected, with high, medium and low amylose contents, with the objective of evaluating the effects of conventional and parboiled rice on glycemia in Wistar rats. The samples with high and medium amylose contents were soaked for 6 h at 65ºC, and the low amylose sample for 7 h at 70ºC. The samples were subsequently autoclaved for 10 minutes at 0.7kgf.cm-2. Six male Wistar rats were used for each treatment. Seven experimental diets were elaborated, formulated according to AIN-93M, the control diet and diets substituting the carbohydrate source with conventional or parboiled rice. Resistant starch was determined in the diets and glycemia monitored using glucose paper strips, the sample being blood obtained from the distal part of the rat’s tail. For the glycemic curve, glycemia was measured in the fasting state and during 90 minutes post-prandial. The results indicated there were no significant differences between the diets formulated with high, medium and low amylose, parboiled or conventionally prepared, with respect to fasting or post-prandial glycemia in Wistar rats.

Análise dos teores de ácidos cianídrico e fítico em suplemento alimentar: multimistura / Analysis of hydrogen cyanide and phytic acid contents in feeding supplements: multimixture

OBJETIVO: Este estudo objetivou quantificar o teor de ácido cianídrico em folhas de mandioca, que receberam tratamento prévio antes da secagem, e a concentração de ácido fítico na multimistura submetida à cocção úmida. MÉTODOS: Utilizou-se a multimistura produzida pela Pastoral da Criança da cidade de Pelotas (RS), constituída por: farelos de trigo (30 por cento) e arroz (30 por cento): farinhas de milho (15 por cento) e trigo (10 por cento); pós de casca de ovo (5 por cento), de folha de mandioca (5 por cento) e de sementes (5 por cento, abóbora ou girassol). Foi realizada orientação ao fornecedor da folha de mandioca sobre a forma recomendada de preparo antes da secagem. RESULTADOS: O conteúdo de ácidos cianídrico e fitatos no suplemento alimentar foram respectivamente de 85mg.kg-1 e 35.90mg.100-1. CONCLUSÃO: Verificou-se que a mudança na forma de secagem das folhas de mandioca foi eficiente para a redução de glicosídeos cianogênicos, e que o processo de torrefação dos ingredientes foi suficiente para produzir a redução de ácido fítico da multimistura aos níveis preconizados pela legislação, não sendo observadas diferenças estatisticamente significantes quando comparadas as amostras que também foram tratadas com calor úmido.

OBJECTIVE: The objective of this study was to quantify the amount of hydrogen cyanide in cassava leaves that were treated before drying and the concentration of phytic acid in a multimixture submitted to wet cooking. METHODS: The multimixture produced by the Pastoral da Criança of the city of Pelotas (RS) consisting of wheat flour (30 percent), rice flour (30 percent), corn flour (15 percent), wheat (10 percent), egg shell powder (5 percent), cassava leaves (5 percent) and pumpkin or sunflower seeds (5 percent) was used. The supplier was advised on how to process the cassava leaves before drying them. RESULTS: The hydrogen cyanide and phytic acid contents of the feeding supplement are 85mg.kg-1 and 35.90mg.100-1 respectively. CONCLUSION: Changing the way the cassava leaves were dried was efficient to reduce the amount of cyanogenic glucosides. The cooking process of the ingredients was enough to reduce the phytic acid content in the multimixture to the levels recommended by the legislation. Significant statistical differences were not observed between these samples and those treated with moist heat.

Ensaio randomizado sobre o impacto da multimistura no estado nutricional de crianças atendidas em escolas de educação infantil / Randomized clinical trial of the impact of a nutritional supplement \"multimixture\" on the nutritional status of children enrolled at preschools

OBJETIVO: Avaliar o efeito da multimistura adicionada à merenda escolar sobre o estado nutricional de crianças atendidas em escolas municipais de educação infantil. MÉTODOS: Estudo de intervenção, longitudinal e controlado, incluindo 24 escolas de educação infantil que foram comparadas antes e depois da intervenção. Os grupos controle e intervenção foram definidos por sorteio das escolas que haviam sido emparelhadas por situação nutricional. A intervenção consistiu em adicionar 10 g de multimistura à alimentação fornecida às crianças que freqüentavam as 12 escolas do grupo intervenção. As medidas de desfecho incluíram as diferenças de escore z dos três índices nutricionais e dos valores de hemoglobina no período de 6 meses em que a suplementação foi fornecida. Modelo multinível foi utilizado nas análises. RESULTADOS: As médias de escore z de peso por idade ao final do acompanhamento foram de 0,40 (±1,34) e 0,31 (±1,32), respectivamente, nos grupos intervenção e controle. A análise em multiníveis mostra diferenças não significativas a favor da intervenção nas médias de escore z de peso para idade (β 0,05; IC95 por cento -0,03 a 0,12) e de estatura para idade (β 0,02; IC95 por cento -0,06 a 0,09). A diferenηa média de hemoglobina foi contrária à intervenção, mas também não foi significativa (β -0,01; IC95 por cento -0,36 a 0,34). CONCLUSÃO: A suplementação com 10 g de multimistura não mostrou efeito significativo sobre nenhuma das medidas ou índices nutricionais estudados em crianças atendidas em escolas municipais de educação infantil.

OBJECTIVE: To evaluate the effect of adding a nutritional supplement "multimixture" to school meals on the nutritional status of children enrolled at municipal preschools. METHODS: Longitudinal, controlled intervention study of 24 preschools which were compared before and after an intervention. The control and intervention groups were defined by drawing lots to choose schools that had previously been paired for nutritional status. The intervention consisted of the addition of 10 g of multimixture to the meals provided to children attending the 12 schools in the intervention group. Outcome measures include changes in z scores for the three nutritional indices and hemoglobin values over the 6-month period during which the supplement was added. A multilevel model was used for analyses. RESULTS: Mean z scores for weight for age at the end of follow-up were 0.40 (±1.34) and 0.31 (±1.32) for the intervention and control groups, respectively. The multilevel analysis demonstrated non-significant differences in favor of the intervention in mean z scores for weight for age (β 0.05; 95 percentCI -0.03 to 0.12) and height for age (β 0.02; 95 percentCI -0.06 to 0.09). Mean change in hemoglobin was against the intervention, but this was also without significance (β -0.01; 95 percentCI -0.36 to 0.34). CONCLUSIONS: Supplementation with 10 g of multimixture did not have a significant effect on any of the nutritional indices or measurements of the municipal preschool pupils studied here.

O processamento doméstico do feijão-comum ocasionou uma redução nos fatores antinutricionais fitatos e taninos, no teor de amido e em fatores de flatulência rafinose, estaquiose e verbascose / The domestic processing of the common bean resulted in a reduction in the phytates and tannins antinutritional factors, in the starch content and in the raffinose, stachiose and verbascose flatulence factors

The objective of this study was to evaluate the effect of the soaking step and the domestic processing of the common bean, on the chemical composition, the levels of phytate, tannin, starch and flatulence factors by utilizing the follows treatments: raw bean (FC), freeze-dried cooked unsoaked bean (FCSM), freeze-dried cooked bean without the non-absorbed soaking water (FCSAM), freeze-dried cooked bean with the non-absorbed soaking water (FCCAM) and the soaking water (AM). The beans were soaking for a period for 16 hours in the proportion 3:1 (water:beans) at room temperature. The effect of the phytates and tannins on the net protein efficiency ratio (NPR) and protein digestibility using male Wistar rats were studied. A decrease in the phytate content of the beans (85) with use of soaking was observed. In the case of the tannin content, only the cooking of the beans promoted high decomposition (84). In the (FCSAM) treatment a decrease in the raffinose (25.0), stachiose (24.8), verbascose (41.7) and starch (26.8) contents was observed. Diets containing casein (control), casein plus the soluble solids obtain from the soaking water showed no significant difference (p > 0.05) for the NPR, as well as for the different bean treatments, although these showing lower values. The treatment (FCSM) showed the higher digestibility (74.3 +/- 5.8) of the bean treatments, the casein diets showing 94.6 +/- 0.9. The reduction of the phytates, tannin, starch contents and flatulence factors in the common bean was most effective when the soaking water not absorbed was discarded (FCSAM).

Teor e estabilidade de vitamina C em sucosin natura e industrializados / Content and stability of vitamin C in industrialized and in natura juices

Buscou-se quantificar e comparar o teor de vitamina C dos sucos frescos in natura e sucos industrializados prontos parabeber, no momento do preparo, na abertura da embalagem e durante o armazenamento refrigerado. Assim como relacionaro teor encontrado com o custo para atingir a recomendação de ingestão diária desta vitamina, para adultos do sexofeminino e masculino. A amostragem foi composta de sucos de laranja, uva, manga, goiaba, tangerina e abacaxi, sendoutilizadas para os industrializados três marcas diferentes. As análises de vitamina C foram efetuadas no momento do preparoou abertura da embalagem, no 4º, 8º e 12º dia, a partir do método de Tillmans. Para verificação dos custos, estes foramrelacionados aos dados de: teor de vitamina C dos sucos, volume de um copo de 200 mL, recomendação de ingestãoe preço gasto na aquisição. Teores superiores de vitamina C foram encontrados na maioria dos sucos frescos in natura,sendo os resultados dos industrializados superiores apenas nos sabores de tangerina (marca B, G) e manga (marca E). Emrelação à estabilidade, não foi possível afirmar qual tipo de suco, natural ou industrializado, apresentou maior estabilidade.O maior custo-benefício foi obtido pelos sucos naturais, dependendo da época de safra da fruta. Desta forma, torna-senecessário ressaltar a importância do consumo de frutas frescas, tanto na forma de suco quanto na sua forma in natura,fator este, comprovado neste estudo.

It was sought to compare the content of vitamin C between fresh in natura and industrialized juices, ready to drink, immediatelyafter being prepared or in the opening package respectively, as well as, during the refrigerated storage. These resultswere also related to the daily recommended intake of the vitamin for female and male adults. The sampling was composedby orange, grape, mango, guava, tangerine and pineapple juices, three different industrialized brands were used one sampleof each, but guava and tangerine were used just two brands of each. The vitamin C tests were performed, using Tillman’smethodology, immediately after the preparation or packing opening and on the fourth, eighth and twelfth following days. Toverify the costs, these data were related: juice vitamin C content, 200 ml cup volume, recommended intake and price paidon the purchase. Higher vitamin C content was found mostly on the fresh in natura juice samples, and the industrializedhigher results were presented in only tangerine (brands B, G) and mango (brand E) flavors. Regarding the stability, it wasnot possible to define which kind of juice, fresh or industrialized showed de higher one. The most favorable cost benefitwas presented on the fresh juices, depending on the fruits harvest. Thereby, is advisable the consumption of fresh fruits, asjuices as well as on its in natura state, as it has been shown on the present study.