Tissue culture of Swertia bimaculata / 中草药

Objective In order to protect the natural resources of Swertia bimaculata which has been destroyed seriously,the method of artificial propagation by way of tissue culture have been systematically studied.Methods The stems,leaves,and stems with buds which were from the seedlings germinated from the seeds on the initial medium were taken as explants.These explants were cultured on MS culture media by adding different portions of hormones at various cultural conditions.Results The stems were the best material in speeding propagation among the three explants.The proper initial medium for the stems was MS+ BA 0.5 mg/L+saccharose 3.0%,the optimum medium for proliferation was MS+BA 0.5 mg/L+IBA 0.1 mg/L+saccharose 3.0%,and the best medium for rooting was 1/2MS+NAA 0.5 mg/L+saccharose 1.5%.Conclusion Tissue culture of S.bimaculata could make its propagation rapid,its resources preserved,and its utilization last.

Tissue culture of medicinal plant Swertia mileensis / 中草药

Objective In order to preserve the natural resources of Swertia mileensis which has been destroyed seriously, the method of artificial propagation by way of tissue culture has been systematically studied. Methods The immature stems and the mature leaves were the best material explants in rapid propagation in all of the experiments. These explants were cultured on MS culture media by adding different portions of hormones at various cultural conditions. Results For the leaves, the suitable phytohormone combination to induce callus are Zt 0.5 mg/L+NAA 0.1 mg/L+IBA 0.1 mg/L or BA 0.5 mg/L+2,4-D 0.1 mg/L+IBA 0.1 mg/L; for the stems, they are BA 0.02 mg/L+Kt 0.04 mg/L+IBA 0.05 mg/L; for the adventitious buds, they are BA 2.0 mg/L+NAA 0.5 mg/L or BA 2.0 mg/L+IBA 0.1 mg/L; and for the roots, they are MS+Kt 0.01 mg/L+IBA 0.5 mg/L+NAA 1.0 mg/L. Conclusion Tissue culture of S. mileensis can make its propagation rapid, its resources preserved, and its utilization last.