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1.
Asian Pacific Journal of Tropical Medicine ; (12): 780-784, 2013.
Artigo em Inglês | WPRIM | ID: wpr-819967

RESUMO

OBJECTIVE@#To compare the sensitivity and specificity of direct fecal smear microscopy, culture, and polymerase chain reaction in the detection of Blastocystis sp. in human stool.@*METHODS@#Human stool samples were collected from a community in San Isidro, Rodriguez, Rizal, Philippines. These samples were subjected to direct fecal smear microscopy, culture and polymerase chain reaction to detect the presence of Blastocystis sp.@*RESULTS@#Of the 110 stool samples collected, 28 (25%) were detected positive for the presence of Blastocystis sp. by two or more tests. Culture method detected the highest number of Blastocystis-positive stool samples (n=36), followed by PCR of DNA extracted from culture (n=26), PCR of DNA extracted from stool (n=10), and direct fecal smear (n=9). Compared to culture, the sensitivity of the other detection methods were 66.7% for PCR from culture and 19.4% for both PCR from stool and direct fecal smear. Specificity of the methods was high, with PCR from culture and direct fecal smear having 97.3%, while PCR from stool at 95.9%.@*CONCLUSIONS@#In this study, in vitro culture is the best method for detecting Blastocystis sp. in human stool samples.


Assuntos
Humanos , Blastocystis , Biologia Celular , Genética , Infecções por Blastocystis , Diagnóstico , Parasitologia , Técnicas de Cultura de Células , Métodos , Fezes , Parasitologia , Microscopia , Métodos , Reação em Cadeia da Polimerase , Métodos , Sensibilidade e Especificidade
2.
Asian Pacific Journal of Tropical Medicine ; (12): 234-238, 2012.
Artigo em Inglês | WPRIM | ID: wpr-819792

RESUMO

OBJECTIVE@#To determine the profiles of anti-Entamoeba histolytica (E. histolytica) IgA, IgG, and IgM in sera of diarrheic and non-diarrheic individuals and partially characterize target antigens.@*METHODS@#Serum samples from thirty diarrheic and thirty non-diarrheic individuals were subjected to IgA, IgG, and IgM profiling through enzyme-linked immunosorbent assay (ELISA), flow cytometry, and immunoblot.@*RESULTS@#ELISA titer results showed that both diarrheic and non-diarrheic individuals possess high levels of E. histolytica-specific IgG compared to IgA and IgM. Flow cytometry data showed that diarrheic serum samples had higher mean reaction percentages against E. histolytica cells compared to non-diarrheic samples. Immunoreactive E. histolytica proteins with molecular weights ranging between 7 kDa and 292 kDa were recognized by diarrheic serum IgG, and 170 kDa and 250 kDa by non-diarrheic serum IgG.@*CONCLUSIONS@#Our findings suggest that serum anti-E. histolytica IgG, compared with serum anti-E. histolytica IgA and IgM responses, was generally high in both diarrheic and non-diarrheic sera, indicating a past exposure to the organism both in symptomatic patients as well as in asymptomatic carriers, respectively. In addition, serum IgG from diarrheic and non-diarrheic patients were able to detect immunogenic E. histolytica proteins.


Assuntos
Adulto , Feminino , Humanos , Masculino , Anticorpos Antiprotozoários , Sangue , Diarreia , Alergia e Imunologia , Entamoeba histolytica , Alergia e Imunologia , Ensaio de Imunoadsorção Enzimática , Citometria de Fluxo , Immunoblotting , Imunoglobulina A , Sangue , Imunoglobulina G , Sangue , Imunoglobulina M , Sangue , Imunoglobulinas , Sangue
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