Bioatividade de Stryphnodendron adstringens, S. Polyphyllum, Caryocar brasiliense, plantas do cerrado brasileiro, sobre Trypanosoma cruzi in vivo / Bioactivity of Stryphnodendron adstringens, S. Polyphyllum, Caryocar brasiliense, plants from brazilian’s savannah on the Trypanosoma cruzi in vivo

O presente trabalho verificou a ação do extrato bruto etanólico da casca do Stryphnodendron adstringens, S. polyphyllum e Caryocar brasiliense, plantas do cerrado brasileiro, sobre a parasitemia de camundongos inoculados com a cepa Y de Trypanosoma cruzi na fase aguda da infecção, avaliando a interferência na agudização ou inibição do processo infeccioso. Os camundongos (Balb/C) foram infectados por via intraperitoneal com 1 x 104 formas tripomastigotas e, após 24 horas, inoculados por via intragástrica com dose diária de 400 micro grama/ml dos extratos durante 10 dias. A avaliação da parasitemia foi realizada em intervalos de dois dias até a morte dos animais. Durante o estudo, observou-se que o extrato do S. adstringens e S. polyphyllum apresentou aumento da atividade tripanocida no sexto e oitavo dias, ocorrendo maior inibição de crescimento no oitavo dia, quando comparado ao grupo controle. O extrato bruto de C. brasiliense apresentou atividade tripanocida com inibição de crescimento apenas no oitavo dia. Os resultados demonstraram que os extratos das plantas testadas apresentaram uma significativa interferência na curva de parasitemia do T. cruzi, reduzindo o número de parasitos no sangue dos camundongos.

The present work verified the action of the crude extract of the Stryphnodendron adstringens, S. polyphyllum and of the Caryocar brasiliense barks, on the parasitemia of mice inoculated with cepa Y of Trypanosoma cruzi in the acute phase of the infection, evaluating its interference on the agudization or inhibition of the infectious process. Mice (Balb/C) had been infected by intraperitoneal route with 1x 104 tripomastigotes forms, after 24 hours inoculated by intragastric route with daily doses of 400 micron gram/ml extracts for ten days. The evaluation of the parasitemia was achieved in intervals of 2 days until the death of the animals. During the study, it was observed that the extracts of S. adstringens and S. polyphyllum had presented a higher trypanomicidal activity after the sixth and eighth days, occurring a higher growth inhibition in the 8 th day, when it was compared to the control group. The crude extract of C. brasiliense presented a trypanocidal activity with growth inhibition only in the eighth day. The results demonstrated that the extracts of the tested plants presented a significant interference on the parasitemia growth of T. cruzi, reducing the number of parasites in the mice blood.

Effect of citrinin and in association with aflatoxin B1 on the infectivity and proliferation of Toxoplasma gondii in vitro

Macrophages exposed to 10 mug/mL citrinin (CTR) or 0.01 mug CTR mixed with 0.04 mug aflatoxin B1 (AFB1) for a period of 2 h at 37ºC, were infected with 10(6) Toxoplasma gondii tachyzoites/muL. The parasites were treated with mycotoxins (2 h at 37ºC) before being added to the macrophage culture. The number of tachyzoites was quantified 2, 24, 48, 72 and 96 h after infection. During the first 2 hours, 59 percent infectivity was observed in the control. After exposure to CTR or the mixture of toxins (CTR-AFB1), macrophages were infected with 77.5 percent and 75 percent of the inoculated tachyzoites, respectively. Similarly, 72.3 percent of the cells were infected when cultured together with previously treated parasites. The treatment with CTR-AFB1 gave rise to 2.9 times more tachyzoites than the control at 72 h. An increased number of parasites was recovered from macrophages exposed to CTR after 96 h, and to CTR-AFB1 after 72 h of culture; The number of tachyzoites recovered from the supernatant was 1.94 and 2.06 times higher, respectively, than in the control (5 x 10(5) ± 0.054 /mL).

Atividade tripanocida in vivo de Stryphnodendron adstringens (barbatimão verdadeiro) e Caryocar brasiliensis (pequi)

O Stryphnodendron adstringens e o Caryocar brasiliensis são espécies nativas do Cerrado e vem sendo testadas como forma alternativa no controle de parasitoses e vetores. Este trabalho avaliou a ação dos extratos bruto etanólicos das cascas destas espécies na concentração de 400 ppm, sobre a parasitemia do Trypanosoma cruzi. Camundongos foram infectados por via intraperitoneal com 1x104 formas tripomastigotas do parasito e após 24 h, inoculados por via intragástrica com dose única diária dos extratos durante dez dias. A avaliação de parasitemia foi efetuada em intervalos de 48 h até a morte dos animais. Os resultados demonstraram que durante as quantificações, os extratos de S. adstringens e de C. brasiliensis apresentaram uma significativa interferência sobre a curva de parasitemia do T. cruzi, reduzindo o número de parasitos no sangue.

Crude extracts from barks of Stryphnodendron adstringens and Caryocar brasiliensis, native species of Cerrado, have been screened at 400 ppm for antiparasitic activity against Trypanosoma cruzi. Mice were inoculated with 1x104 parasites, after 24 h they were treated with one day dose extracts during ten days. The trypanosomicidal activity was evaluated each 48 h until animals death. The extracts of S. adstringens and C. brasiliensis decreased significantly the number of parasites in the blood.