Study of production of inactivated influenza vaccine for human on egg-grown from reassortants NIBRG-14 at vaccine institute

Background: A/H5N1 influenza virus spreads from birds to humans and cause influenza diseases with high mortality rate. Vaccination is the most effective way to protect communities from pandemic, reduce morbidity and mortality. The study of creating A/H5N1 influenza vaccines in conformity with Vietnam was the urgent need. Institute of Vaccine\u2019s Achievement (IVAC) studied production of inactivated influenza vaccine for human on egg-grown from reassortants NIBRG-14. Objectives: In order to produce experimentally A/H5N1 influenza vaccine for human in accordance with WHO requirements and set up a viable process for production of the vaccines. Subjects and method: 10 days embryonated eggs and NIBRG-14 strains were served to the study with LAL method to check endotoxin, Kijehdal method to test total protein. Results: IVAC had produced successfully 5 lots of absorbed vaccine A/H5N1 (FLUVAC) using NIBRG-14 strains and embryonated eggs. Initially, production and quality control processes had been set up at IVAC by applying the recommendations of WHO. Conclusion: The success of the study was a basis of the approval of the government to establish a influenza vaccine manufacturing facilities.

Experiment with different adjuvants for HI5N1 influenza vaccine

Background Nha Trang Institute of Vaccines Medical Biologicals has produced successfully vaccine for human influenza A/H5N1 in embryonic chicken eggs. Effectiveness of the vaccine immune response is highly dependent on the type of adjuvant used.\r\n', u'Objective: To study adjuvants for preparing A/H5N1 influenza vaccine.\r\n', u'Subjects and methods: The study used a comparative approach on capacity to increase the immune response of various adjuvants AIPO4, Chitosan and Freund tested in mice. Experiment of erythrocyte agglutination reaction is used to measure levels of antibody response in mice blood. The process of weight gain in mice after injection of vaccine adjuvant mixture is monitored.\r\n', u'Results: HA antibody level of the vaccine adjuvant mixture is higher than of the vaccine without adjuvant. Capacity of stimulating immune response of Chitosan adjuvant is higher than AlPO4. Repeat injection on day 20th is suitable for vaccine with AlPO4or vaccine without adjuvant. Repeat injection is after day 20th for vaccine with Chitosan or Freund depending on the amount of residual antibody in the blood. \r\n', u'Capacity of stimulating immune response of Freund adjuvant is the best in the 3 adjuvants but the safety of this adjuvant is low\r\n', u' Freund adjuvant should be used only for animals. When using Freund adjuvant to induce immunity to animals it should determine the amount of antibodies causing immune before repeating.\r\n', u'Conclusion: Freund adjuvant should be used only for animals. \r\n', u'\r\n', u'

Primary studies about the effect of oligo-alginate on the growth and the development process of bacillus subtilis

The supplementation of oligo-alginate with molecular weight of 4.700 Dalton at the concentration of 30-50 ppm has made the biomass of Bacillus subtilis increased visibly.

The prospect of applying radiation technology cuting polysaccharide to produce new generation vaccine

During last 2 decades, the need of new generation vaccine from encapsulated bacterial polysaccharide for preventing typhoid, pneumonia, Hib and menigococcal diseases… rose. Chemical vaccine has high safety, efficiency even in young children. Polysaccharide (conjugated) and making an ability to combine different antigens could prevent many diseases. This is the right way to reduce the times of injection and lower the prices to serve well

Use of caprylic acid in purification of rabies antiserum

Study on the use of caprylic acid in the purification of anti rabies serum. The use of coprylic acid (octanoic) in the cleaning process IgG from horse plasma was studied to apply in the purification of anti rabies serum in the Institute of Vaccine and Biological Substances. When using caprylic acid in the purification of serum, the quality and quantity of the products were improved. The purity and colour of product was managed as desire. The process had a high effectiveness and short time of purification