RESUMO
Background: Pseudomonas aeruginosa is an important pathogen isolated from various clinical samples. It continues to cause complication in nosocomial infections
Objective: To determine the prevalence and antibiotics resistance pattern of P.aeruginosa isolated from a clinical samples at a tertiary care hospital
Methodology: This was a cross sectional study. A total of hundred clinical samples comprising urine, pus, blood and wound swabs were collected from ICU and burn unit, surgical ward, medical ward and OPD of Sheikh Zayed Medical College/Hospital, Rahim Yar Khan. These samples were cultured on MacConkey and Blood agar. Urine was cultured on CLED agar. Plates were aerobically incubated at 35-37 [degree sign]C. Positive cultures were identified by culture characteristics and biochemical reactions. Antimicrobial resistance of all isolated bacteria was done by the disk diffusion testing. Pure culture was obtained then inoculated on Nutrient agar plate using disks of amoxicillin: clavulanic acid, ceftazidime, ciprofloxacin, amikacin, imipenem, piperacillin tazobactam and gentacin. After 24 hrs incubation plates were examined to read the inhibition zones
Results: From these samples Pseudomonas aeruginosa was isolated from urine [17%], pus [26%], blood [9%] and wound swab [48%]. Pseudomonas aeruginosa showed resistance against Piperacillin Tazobactam [99%], Amoxicillin Clavulanic Acid 91%, Tigecycline 89%, Amikacin 83% and Ceftazidime 79%
Conclusion: Our Study showed that P. Aeruginosa, is the common microorganisms isolated resistance pattern is against common antibiotics. To cope with antimicrobial resistance against critically ill patients with pseudomonas infections it is necessary to follow firm antibiotic policies and SOPs while implementation of surveillance programmes for MDR bacteria and infection control and prevention procedures are needed
RESUMO
To assess spinal-cord injury patients for the presence of psychological problems and the side effects on the quality of life [QoL]. A prospective cross-sectional study was conducted in three different rehabilitation centers in Islamabad and Rawalpindi, Pakistan among 50 volunteers [32 males and 18 females] ranging from 20 to 45 years in age. WHO QoL-BREF 1996 was used to assess QoL. The presence of psychological problems was recorded using Hospital Anxiety Depression Scale [HADS] and General health Questionnaire [GHQ-12]. The duration of illness was 1-2 years. 32 were diagnosed as primary traumatic spinal cord injury, 16 as non-traumatic spinal cord lesion and two as stock. 15 of the subjects had stress disorder, 21 anxiety disorder and 14 depressions. Patients with spinal cord injury, a prolonged disability requiring inpatient rehabilitation, have impaired QoL that affects all life domains. There is high prevalence of psychological problems in these patients. Results show QoL does affect functional abilities of the patients, but there is no significant relationship between psychological problems and functional abilities