RESUMO
No abstract available.
Assuntos
Neurofibromatoses , Neurofibromatose 1 , Neurofibromina 1 , VitiligoRESUMO
BACKGROUND: Trichophyton(T.) rubrum is most common fungal pathogen that causes tinea pedis and onychomycosis. It recurrently infects human and usually persists for very long time, provoking public health concern. Due to the limitation in current treatment options, alternative therapies are desirable. We investigated the inhibitory effect of UVC, terbinafine hydrochloride 1% and paeonia natural extracts on T. rubrum in vitro. OBJECTIVE AND METHOD: Total 25 T. rubrum strains were cultured for 10 days on Mycosel agar plate; 5 strains of T. rubrum and 5 copies for each strain. They were divided into 5 groups: control, UVC irradiation, terbinafine spray, paeonia natural extracts spray, UVC and paeonia natural extracts sprays. The cultured media were irradiated for 1 hour daily for 3 weeks in the germicidal lamp emitting 253.7 nm (UVC), power of 2.875 mW/cm2 at 10 cm distance. Terbinafine and paeonia extracts was sprayed twice on the surface to fully cover the colony area. The median diameter of each colony were measured every other day for 3 weeks. The change of colony diameter and the growth rate were analyzed. RESULTS: The UVC had virtually no effect on restraining the growth of T. rubrum, similar with the growth of the control group. However, both the terbinafine spray and paeonia extracts slowed down the growth rate remarkably and showed a similar effect. CONCLUSION: We could only figure out the fungistatic effect, and not the fungicidal effect of paeonia extract and terbinafine hydrochloride in vitro. UVC irradiation setting in this study was totally ineffective. More studies are needed on more variable wavelength and the fluence of UVC irradiation. In addition, further verification on the mechanism and the effect of anti-fungal activity by paeonia extracts are needed.
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Humanos , Ágar , Terapias Complementares , Onicomicose , Paeonia , Saúde Pública , Tinha dos Pés , TrichophytonRESUMO
Tinea incognito is a dermatophytic infection with an atypical clinical presentation caused by previous treatment with topical or systemic steroids, as well as by the topical application of calcineurin inhibitors such as pimecrolimus and tacrolimus. The diagnosis of tinea incognito is frequently delayed or missed due to loss of typical appearance. Nowadays, as the use of steroid and topical calcineurin inhibitor has been increasing, the incidence of tinea incognito has also increased. Here we report a case of tinea incognito in a 19-year-old girl initially misdiagnosed as irritant contact dermatitis who also had atopic dermatitis and onychomycosis on the left 3rd finger nail. On the basis of the detection of hyphae on KOH examinations and isolation of Trichophyton rubrum by fungus culture, she was successfully treated with oral terbinafine 250 mg/day and topical terbinafine for 12 weeks.
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Feminino , Humanos , Adulto Jovem , Calcineurina , Dermatite Atópica , Dermatite de Contato , Diagnóstico , Dedos , Fungos , Hifas , Incidência , Onicomicose , Esteroides , Tacrolimo , Tinha , TrichophytonRESUMO
No abstract available.
Assuntos
Carcinoma de Célula de Merkel , Linfonodos , Linfocintigrafia , Metástase NeoplásicaRESUMO
BACKGROUND: Transgenic research on metalloproteinase-1 is an emerging field in the area of plant molecular biology. The new method reported here can similarly be applied in fungal molecular biology to identify different dermatophytes. Our method is more accurate than traditional methods such as molecular analyses. OBJECTIVE: To identify Trichophyton rubrum, T. mentagrophytes var. mentagrophytes, T. tonsurans, T. mentagrophytes var. interdigitale, Microsporum canis and M. gypseum, by using the restriction fragment length polymorphism (RFLP) analysis and polymerase chain reaction (PCR) to detect polymorphisms in the metalloproteinase-1 gene (MEP1). METHODS: From each fungal strain, we isolated genomic DNA and performed PCR to amplify the region coding for metalloproteinase-1. Primers for the metalloproteinase-1 gene were designed based on the sequence in NCBI GenBank. Subsequently, we purified the amplified PCR product and performed RFLP analysis. After restriction enzyme digestion, BsrDI (NEB, England), the samples were subjected to electrophoresis. Four different patterns of DNA fragments were observed among 6 fungal species. RESULTS: The DNA fragments for T. mentagrophytes var. mentagrophytes, T. mentagrophytes var. interdigitale and T. tonsurans showed similar patterns on electrophoresis and were not distinguishable, whereas T. rubrum, M. canis, and M. gypseum showed different patterns. CONCLUSION: To our knowledge, it is the first study to introduce the analysis of the nucleotide sequence of metalloproteinase-1 enzyme to study differentiation in dermatophytes. Based on our results, more accurate differentiation and subtyping of T. rubrum and T. mentagrophytes var. interdigitale might be possible. This might contribute to better understanding of the epidemiology and pathogenesis of dermatophyte.
Assuntos
Arthrodermataceae , Sequência de Bases , Codificação Clínica , Bases de Dados de Ácidos Nucleicos , Digestão , DNA , Eletroforese , Epidemiologia , Metaloproteases , Microsporum , Biologia Molecular , Plantas , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de Restrição , TrichophytonRESUMO
PURPOSE; To evaluate the effects of the chitosan-TGF-beta(1) conjugate and TGF-beta(1) on osteoarthritic rabbit cartilage induced by collagenase type II. MATERIALS AND METHODS; Chemical arthritis was induced in right knee of 15 rabbits by injection of collagenase type II (4 mg/mL, 0.25 cc) twice at day 1 and 4, and then each 5 rabbits were treated by intra-articular injection of TGF-beta(1) (15 ng/mL, 1 mL, group I), chitosan-TGF-beta(1) conjugate (Chitosan: 5.8 g/mL, TGF-beta(1): 15 ng/mL, group II) and PBS (1 mL, group III) at 10 days later in the same knees. The appearance of the injected knee joints and lameness were observed weekly. The articular cartilage and synovium obtained at 4 weeks were analysed by gross findings (India ink), histological examinations (H&E, Safrainin O) and evaluation through the Kikuchi score. RESULT; The osteoarthritic sign about knee joints was the most severe at 1 weeks after the initiation of collagenase injections, but there wasere no difference of 3 groups in osteoarthritic sign and lameness was most severe in group 3. Group 2 showed the most superior results in gross findings using india ink and the these results were more prominent in the evaluation of histological scoring (average scores of each group I:14+/-2.7, II:10.3+/-1.2, III:20.3+/-3.7). CONCLUSION; These results might suggest that, in the rabbit osteoarthritic knee model induced by collagenase, Chitosan-TGF-beta(1) conjugate is more effective than only TGF-beta(1) in inhibiting cartilage degeneration.