RESUMO
Background: Selection of the best embryo for transfer is very important in assisted reproductive technology [ART]. Using morphological assessment for this selection demonstrated that the correlation between embryo morphology and implantation potential is relatively weak. On the other hand, aneuploidy is a key genetic factor that can influence human reproductive success in ART
Objective: The aim of this lab trial study was to evaluate the incidence of aneuploidies in five chromosomes in the morphologically high-quality embryos from young patients undergoing ART for sex selection
Materials and Methods: A total of 97 high quality embryos from 23 women at the age of 37or younger years that had previously undergone preimplantation genetic screening for sex selection were included in this study. After washing, the slides of blastomeres from embryos of patients were reanalyzed by fluorescence in-situ hybridization for chromosomes 13, 18 and 21
Results: There was a significant rate of aneuploidy determination in the embryos using preimplantation genetic screening for both sex and three evaluated autosomal chromosomes compared to preimplantation genetic screening for only sex chromosomes [62.9% vs. 24.7%, p=0.000]. The most frequent detected chromosomal aneuploidy was trisomy or monosomy of chromosome 13
Conclusion: There is considerable numbers of chromosomal abnormalities in embryos generated in vitro which cause in vitro fertilization failure and it seems that morphological characterization of embryos is not a suitable method for choosing the embryos without these abnormalities
RESUMO
In this research, dried acetoneichloroform extract of aerial parts of E. denticulata as one of the endemic plants to Iran, afforded a number of triterpenes and steroids including: betulin, 24-methylene-cycloart-3-ol, cycloart-23Z-ene-3beta,25-diol, cycloart-23E-ene-3beta,25-diol, ergosta-8,24-dien-3-ol [obtusifoliol] and beta-sitosterol which were reported for the first time from this plant. The structure of these compounds was elucidated by NMR and mass spectroscopic methods. The MTS assay was used to determine the toxicity and antiviral activity of betulin and [3/beta,23E]-cycloarta-23-ene-3,25-diol. Betulin showed anti-HSV-1 activity with EC50 value of 84.37 +/- 0.02 microg/mL, and toxicity on normal vero cells with CC50 value of 660.718 +/- 0.072 microg/mL. [3beta,23E]-Cycloarta-23-ene-3,25-diol showed antiviral effect with EC50 value of 86.63 +/- 0.03 microg/mL, and toxicity with CC50 value of 1089.2 H0.25 microg/mL. The results revealed that these two compounds have the antiviral activity far below the CC50 value with selectivity index [CC50/EC50] values of 7.83, and 12.57, respectively