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Introduction:Diazinon (DZN) administration produces lipid peroxidation as an indicator of oxidative stress in the brain. Some medicinal plants such as Doremaglabrumhas antioxidant properties, so can be usedas an antioxidant that may protect neurons from oxidative stress. The aim of present study was to investigate the effect of D.glabrumagainst DZN-induced oxidative stressin hippocampus.Methods:Twenty-four adult male Wistar rats were used in this study.The rats randomly were divided into four groups including a control group, and two groups received different doses of D.glabrum(40 and 80 mg/kg) as pre-treatment for 21 days with DZN (100 mg/Kg) that was injected intraperitoneally (ip) in last day of D. glabrumusage, and one group received only DZN. Thiobarbituric acid reactive substances (TBARS), which are the indicators of lipid peroxidation, and the activities of antioxidant enzymes (glutathione peroxidase, superoxide dismutase and catalase) were determined in the ratsʼhippocampus.Results: Administration of DZN significantly increased TBARS levels and superoxide dismutase activity and decreased glutathione peroxidase activity but there were no significant changes in catalase activity in the hippocampus. Combined D.glabrumand DZN treatment, caused a significant increase in glutathione peroxidase, a significant decrease of TBARS and a significant decrease in superoxide dismutase and again no significant changes in catalase activity in the rats’ hippocampus when compared to the rats treated with DZN.Conclusion:Our study demonstrated that D.glabrumhad an amelioratory effect on oxidative stress induced by DZN
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Background and Objectives: The aim of this study was to evaluate the effects of ethanolic extract of saffron on oxidative stress markers in the hippocampus of experimental models of MS
Materials and Methods: Induction of Multiple Sclerosis [MS] was carried out by direct single injection of 0.01% ethidium bromide [EB] into the Cornu ammonis [CA1] of hippocampal formation. One week after MS induction, animals received intrahippocampal [5 microg/rat and 10 micro g/rat] injection of the saffron. At the end, the bilateral hippo campi were dissected to measure oxidative stress parameters. one-way ANOVA followed by Tukey test were used for Statistical analyses activity
Results: Hippocampal injection of EB had no effect on catalase [CAT] activity, but it induced significant increment of antioxidant enzymes GPx and SOD [P<0.05]. Short-term local injection of saffron extract significantly reduced the activity of GPx and SOD enzymes [P<0.05]. But the activity of CAT was significantly increased following microinjection of saffron extract [P<0.05]
Conclusion: The results of the present study show that the local injection of EB may cause increased production of free radicals. Antioxidant capacity and activity are increased. Saffron extract as a potent antioxidant modulates oxidative stress markers, probably through scavenging of ROS and clearing of cells milieu from free radicals