Dronedarone hydrochloride enhances the bioactivity of endothelial progenitor cells via regulation of the AKT signaling pathway

Cardiovascular disease (CVD) and its complications are the leading cause of morbidity and mortality in the world. Because of the side effects and incomplete recovery from current therapy, stem cell therapy emerges as a potential therapy for CVD treatment, and endothelial progenitor cell (EPC) is one of the key stem cells used for therapeutic applications. The effect of this therapy required the expansion of EPC function. To enhance the EPC activation, proliferation, and angiogenesis using dronedarone hydrochloride (DH) is the purpose of this study. DH received approval for atrial fibrillation treatment and its cardiovascular protective effects were already reported. In this study, DH significantly increased EPC proliferation, tube formation, migration, and maintained EPCs surface marker expression. In addition, DH treatment up-regulated the phosphorylation of AKT and reduced the reactive oxygen species production. In summary, the cell priming by DH considerably improved the functional activity of EPCs, and the use of which might be a novel strategy for CVD treatment.

Chemical constituents and biological activities of essential oils of Amomum genus (Zingiberaceae)

Amomum Roxb. includes the aromatic and medicinal plants native to tropical and subtropical Asia belonging to the family Zingiberaceae. Members of Amomum genus have been used for a long time in traditional medicine for the treatment of throat trouble, congestion of lungs, inflammation of eyelids, and digestive disorders, etc. Amomum essential oils have been studied for their chemical profiles in which limonene, allo-aromadendrene, 1,8-cineole, camphor, farnesyl acetate, α-pinene, β-pinene, caryophyllene, camphene, D-camphor, santolina triene, methyl chavicol, bornyl acetate, β-elemene, δ-3-carene, etc. were the major compounds. Furthermore, the oils extracted from Amomum plants have been reported to possess antimicrobial, antioxidant, insecticidal, larvicidal, cytotoxic, anti-scabies, and anti-inflammatory activities. This review focuses on the chemical constituents and biological activities of the essential oils isolated from the different plant parts of Amomum plants. The objective of the present review is to highlight therapeutic potentials and provide evidence for future medicinal applications of these species of genus Amomum.

Dronedarone hydrochloride enhances the bioactivity of endothelial progenitor cells via regulation of the AKT signaling pathway

Cardiovascular disease (CVD) and its complications are the leading cause of morbidity and mortality in the world. Because of the side effects and incomplete recovery from current therapy, stem cell therapy emerges as a potential therapy for CVD treatment, and endothelial progenitor cell (EPC) is one of the key stem cells used for therapeutic applications. The effect of this therapy required the expansion of EPC function. To enhance the EPC activation, proliferation, and angiogenesis using dronedarone hydrochloride (DH) is the purpose of this study. DH received approval for atrial fibrillation treatment and its cardiovascular protective effects were already reported. In this study, DH significantly increased EPC proliferation, tube formation, migration, and maintained EPCs surface marker expression. In addition, DH treatment up-regulated the phosphorylation of AKT and reduced the reactive oxygen species production. In summary, the cell priming by DH considerably improved the functional activity of EPCs, and the use of which might be a novel strategy for CVD treatment.

Chemical constituents and biological activities of essential oils of Amomum genus (Zingiberaceae)

Amomum Roxb. includes the aromatic and medicinal plants native to tropical and subtropical Asia belonging to the family Zingiberaceae. Members of Amomum genus have been used for a long time in traditional medicine for the treatment of throat trouble, congestion of lungs, inflammation of eyelids, and digestive disorders, etc. Amomum essential oils have been studied for their chemical profiles in which limonene, allo-aromadendrene, 1,8-cineole, camphor, farnesyl acetate, α-pinene, β-pinene, caryophyllene, camphene, D-camphor, santolina triene, methyl chavicol, bornyl acetate, β-elemene, δ-3-carene, etc. were the major compounds. Furthermore, the oils extracted from Amomum plants have been reported to possess antimicrobial, antioxidant, insecticidal, larvicidal, cytotoxic, anti-scabies, and anti-inflammatory activities. This review focuses on the chemical constituents and biological activities of the essential oils isolated from the different plant parts of Amomum plants. The objective of the present review is to highlight therapeutic potentials and provide evidence for future medicinal applications of these species of genus Amomum.

A comparative study of effects of Boneceramic and Bio-Oss on osteogenic differentiation of dog bone marrow mesenchymal stem cells in vitro / 中国药理学通报

Aim To compare the effects of BoneCeramic and Bio-Oss on the proliferation, adhesion and osteogenic differentiation of dog bone marrow mesenchymal stem cells (DBMSCs) in vitro. Methods DBM-SCs were isolated and cultured in vitro and identified for osteogenic and adipogenic differentiation. CCK-8 was used to detect the effects of BoneCeramic and Bio-Oss bone meal extracts on the vitality of DBMSCs. SEM was used to observe the adhesion of DBMSCs on the surface of bone meal. ALP was used to quantitatively detect the osteogenic differentiation of DBMSCs on the surface of bone meal, and the relative expressions of OPN, OCN, RUNX-2, and ALP genes were detected by RT-PCR. Results The cells isolated and cultured from dog bone marrow conformed to the characteristics of mesenchymal stem cells. CCK-8 results showed that the stock solutions of Bio-Oss and Bone Ceramic had no obvious cytotoxic effect on DBMSCs. SEM showed that DBMSCs were distributed more widely on the surface of BoneCeramic than that of Bio-Oss. ALP quantitative detection showed that DBMSCs on the BoneCeramic surface were more conducive to its osteogenic differentiation than Bio-Oss, and it also increased the expression of OPN, OCN, RUNX-2 and ALP genes. Conclusions In vitro studies show that both Bio-Oss and BoneCeramic have good biocompatibility. Compared with Bio-Oss bone meal, BoneCeramic is more conducive to the osteogenic differentiation of DBMSCs.

Evaluating the implementation of national benchmarks in health care at commune level in the period 2001-2010

This study was aimed to evaluate the implementation some issues such as socialization of public health care and prevention, health communication and education at commune level during 2001-2010 period. Results: 88.4% of commune had Committee for primary health care that was operating; the goal of 100% commune had Committee for primary health care could be achieved by the year 2005 in the Red River Delta, Mekong River Delta and rural are of the North Central Coast; and by the year 2010 in the Northwest, South Central Coast and Southeast. There were 22% communes had health funds, with the highest rate in North Central Coast (45.5%), 70-80% commune had staffs for health communication and education. It is needed to make policies for implementing national health standards.

Primary study on chemical constituents of Lasia spinosa L

Lasia spinosa L. (Araceae) has been used in the Vietnamese traditional and folk medicine for treating sore throat, cough, nephritis, rheumatism, liver diseases (hepatitis, ascitic cirrhosis), sequels of malaria and skin diseases. In this primary study on chemical constituents of Lasia spinosa L., leaf powder and root powder of Lasia spinosa L. were leached with n-hexan, CHCl3 and MeOH solvents, three times for each solvent. 5 compounds including p-hydroxy benzaldehyde, acid p-hydroxybenzoic, acid 3-hydroxy-4-methoxy benzoic, 2-(4’- methoxyphenyl)-ethanol, adenine were isolated by spectroscopic methods

First results in chemical study of Fissistigma capitatum Merr. ex Li

Study on chemical components of Fissistigma capitatum Merr. ex Li (scientific name was defined by Bachelor Ngo Van Trại, National Institute of Medicinal Materials) collected at Sa Pa in June 2003. 600g of dried and grinded leaves and branches were leached with methanol:water (95:5) at room temperature. Methanol solvent was distilled at decreased pressure. The remained fluid was extracted many times with n-hexane, ethyl acetate and butanol. N-hexane was vacuum-evaporated, which collected 11.2g sediment. N-hexane sediment fluid was refined by silica gel chromatographic, which collected compounds 1 and 2. The structure of compound 1 was taraxerol, compound 2 was 16-hentriacontanone with white crystal form when comparing with document data. This compound named palmitone and previous isolated from the leaf of Annona diversifolia

The real state of knowledge and practice on reproductive health care in married women aged 15 to 49 years

In October 2002, a study was carried out in 2 communes Dan Tien and Cuc Duong, Vo Nhai district, Thai Nguyen province, concerning the knowledge and practice of reproductive health care in married womens, aged 15-49 years old. Almost pregnant women were aware that it takes 3 times to frequent the maternal clinics, to give breast feeding early immediately after child birth, and the breast feeding prolonged enough. However, in practice, only 75.6% and 78.1% in 2 above mentioned communes respectively. Pregnant women underwent the pregnancy examination and had got 3 times enough to get examination in the whole their pregnant period. 50% and 52% pregnant women had been given antitetanic infection in their pregnant period. 46% and 31% had had proper breast feeding, 84.7% and 61.3% gave birth in state health institutions.

Study on the changes of biochemical index in rabbit blood before and after receiving "Thap toan dai bo" tablet

Two spectices of health Rabbit weighing 1,8-2,5 kg were descriptive in comparing before and after studying. Oral administration with "thap toan dai bo" pills- a traditional medicine, in experimental showed that the pill haft an effect to vary some biochemical indices of rabbit. However these variation did not influence on the normal metabolism of the animal as well as on the biochemical function, for instance in glucose with the comparative coefficients of P>0,05, proted P>0,05 and some other indices of chlolesterol, triglycerid… with P>0,05. These comparative coefficient had not statistical significance.

To study some risk factors of cerebrovascular accident complications

In ThaiNguyen Central General Hospital some cerebro-vascular complications were studied. Common risk factors leading to complications were: Hypertension with the highest incidence of 78,5%, lipidemia disorder 53,9%, alcoholism 26,9%, tabagism 21,4%, catching a cold 16,4%, transient ischaemia 14,8%, familly factors10,9%, heart diseases 8,2%, stress 5,4% and diabetes 3,8%.

The variation of blood biochemical indices before and after treating Basedow’s disease using propyl thiouracyl

After a 4 weeks of treatment by propyl thyouracil, the biochemical indices of Basedow disease had changed obviously: triiodothyronin (T3) high level (7.95 ± 1.58 nmol/l before treatment) had increased dramatically (1.98 ± 0.54 nmol/l), total thyroxin (T4) high level (257.56 ± 47.45 nmol/l before treatment) had decreased dramatically (1278.04 ± 10.82 nmol/l). FT4 serum level high level had decreased after treatment. TSF low level before treatment had increased. But after treatment glucose level had increased unsignificantly versus that before. In severe condition blood cholesterol had increased, but in moderate and mild condition, there was no change.

Preliminary results of the topic formula of “Kho tri tan” in combining with oral use of traditional medicinal plant in treating anal hemorrhoid

35 patients with internal hemorrhoid of II, III, IV grade were treated by simple technique in a short duration and thourough operation without complicate equiments, the traditional formule “kho tri tan E” in combining with Vietnamese traditional herbal medicines gave preliminary satisfied efficacy through the evaluation of 2 main criteria of treatment as bleeding severity and the fall of hemorrhoid knot. Before the treatment, 100% of patients had got bleeding of diverse levels. After the treatment, there was 14.3% of patients still had got mild bleeding. In 100% of treated cases, the whole of hemorrhoid knots falled down. No complication occurred.

Sensitivity and specificity of glycolipid antigens in serodiagnosis of tuberculosis

To find out specific antigens to M. tuberculosis, the researchers searched for sensitivity, specificity and cross reactions of 6 glycolipid antigens which were extracted and purified from M. tuberculosis with 168 sera by ELISA. The 168 samples were divided into 3 groups: Group I: 62 lung tuberculosis patients with AFB-smear positive; group II: 53 healthy persons and group III: 53 infectious other than tuberculosis patients. There were 6 glycolipid antigens were used in this study, they were Phosphoinositomannoside (P2), 6, 6’-dimycolyltrehalose (cord factor) (DT), Trehalose monomycolate (MT), Phenol glycolipid (PA), Glycolipid (MI) and Glycopeptide (Co). The sensitivity and specificity of DT. MT and PA antigens were 74.2; 66.1; 69, 4% and 83.0; 69.8%; 73.6%, respectively; cross reaction ranging from 22.6% to 39.6%. The sensitivity and specificity of other antigens were lower and its cross reactions were higher than DT, MT and PA antigens. ELISA with specific glycolipid antigens can be used to diagnose extra-lung tuberculosis and lung tuberculosis with AFB(-).

Cloning and sequencing of the gene coding for diphtheria toxin from corynebacterium diphtheriae vaccine strain at IVAC

In this study, PCR technique was used with 2 primer pairs for amplifying DNA fragments at 5’ and 3’ of the gene encoding diphteria toxine of the strains Corynebacterium diphtheriae. After the cloning and sequencing, 2 separated fragments were joined to form a complete gene. The sequence of gene was translated into protein and the results were submitted into Gene Bank Database

Immunohistochemical study of primary extranodal malignant non-Hodgkin's lymphoma

Between 1996 and 1998, 31 cases of primary extranodal malignant non-Hodgkin's lymphoma were studied immunohistochemically. Immunohistochemical study showed that the majority of the cases were of B-cell type: 19 cases (73.07%) and only 3 cases (11.53%) were of the T-cell type. According to the Working Formulation system, the most common histologic type was diffuse, large cell type (61.29%). The histologic grade was moderate grade: 23 cases (74.19%), high grades: 5 cases (16.13%).