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1.
National Journal of Andrology ; (12): 115-121, 2012.
Artigo em Chinês | WPRIM | ID: wpr-238978

RESUMO

<p><b>OBJECTIVE</b>To investigate the possibility of applying multiplex ligation-dependent probe amplification (MLPA) to the detection of azoospermia factor (AZF) microdeletion on the Y chromosome in infertile men with azoospermia or severe oligozoospermia.</p><p><b>METHODS</b>DNA samples were obtained from 147 azoospermia or severe oligozoospermia patients and 154 normal controls. After denatured at 95 degrees C, the samples were hybridized to the specific probes designed for the AZF region. With the ligase, the hybrid products were amplified by a pair of universal primers labeled with FAM fluorescence, and then separated by capillary electrophoresis for data analysis. Meanwhile all the samples were subjected to multiplex-PCR (mPCR) analysis for sequence-tagged sites (STS) in the AZF region.</p><p><b>RESULTS</b>STS deletion was detected in 22 (15.0%) of the 147 patients but not in the normal controls. By MLPA, 40 (27.2%) of the patients were found with specific probe omission in the AZF region, as compared with 20 cases in the control group.</p><p><b>CONCLUSION</b>Compared with mPCR, MLPA has a better sensitivity in detecting AZF microdeletions, and it provides more precise genetic information on the AZF regions, which may contribute to in-depth exploration into the etiological mechanism of impaired spermatogenesis.</p>


Assuntos
Adulto , Humanos , Masculino , Adulto Jovem , Azoospermia , Genética , Estudos de Casos e Controles , Deleção Cromossômica , Cromossomos Humanos Y , Genética , Sondas de DNA , Loci Gênicos , Infertilidade Masculina , Técnicas de Amplificação de Ácido Nucleico , Métodos , Oligospermia , Genética , Reação em Cadeia da Polimerase , Métodos , Proteínas de Plasma Seminal , Genética , Sitios de Sequências Rotuladas , Aberrações dos Cromossomos Sexuais , Transtornos do Cromossomo Sexual no Desenvolvimento Sexual , Genética
2.
National Journal of Andrology ; (12): 750-755, 2006.
Artigo em Chinês | WPRIM | ID: wpr-343529

RESUMO

<p><b>OBJECTIVE</b>To investigate the efficacy of the Chinese medicine Huzhangdanshenyin on male immune infertility, and provide an effective therapy for this disease.</p><p><b>METHODS</b>Ninety men with immune infertility were selected as the research subjects and randomly divided into two groups, 60 in the treatment group, treated by Huzhangdanshenyin, and the other 30 in the control, treated by prednisone, both for 3 months. The improvement of clinical symptoms, immunologic indexes (antisperm antibodies in serum and seminal plasma) and sperm indexes (semen liquefied duration, motility, viability, density and abnormal morphology rate) were observed and the results analyzed.</p><p><b>RESULTS</b>The total antisperm antibody reversing ratio of the treatment group was higher than that of the control (P < 0.01), especially the serum antisperm antibody reversing ratio. There were significant differences in the clinical cure rate and total validity rate between the treatment group and the control (P < 0.01). After the treatment, the markers of the clinical symptoms (P < 0.01), especially the serum antisperm antibody reversing ratio. There were significant differences in the clinical cure rate and total validity rate between the treatment group and the control (P < 0.01). After the treatment, the markers of the clinical symptoms were lower (P < 0.01), and the improvement of the clinical symptoms was better in the treatment group than in the control (P < 0.01), especially the symptoms of pain in the back and knees, distending and bearing-down sensation of the perineum and testis, hypersexuality and topalgias. Compared with pre-treatment, sperm motility and viability of the treatment group significantly improved (P < 0.01), and so did sperm density (P < 0.05). However, there were no significant differences in sperm density, semen liquefied duration, abnormal morphology rate and pH (P > 0.05) before and after the treatment.</p><p><b>CONCLUSION</b>The Chinese medicine Huzhangdanshenyin works more effectively than prednisone in the treatment of male immune infertility. It could improve the antisperm antibody reversing ratio, clinical symptoms and signs and ameliorate sperm indexes with no obvious advierse effects.</p>


Assuntos
Adulto , Humanos , Masculino , Pessoa de Meia-Idade , Autoanticorpos , Metabolismo , Medicamentos de Ervas Chinesas , Usos Terapêuticos , Infertilidade Masculina , Tratamento Farmacológico , Alergia e Imunologia , Fitoterapia , Contagem de Espermatozoides , Motilidade dos Espermatozoides , Espermatozoides , Alergia e Imunologia
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