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Objective To explore the curative effect and safety of L-carnitine combined with alprostadil for patients with diabetic nephropathy.Methods 102 patients with diabetic nephropathy were enrolled in hospital of Inner Mongolia normal university from August 2013 to December 2016,of which patients divided into two groups randomly,control group (n =50) accepted alprostadil treatment,and combined group (n =52) adopted L-camitine based on the patients in control group.The blood glucose and renal functions were detected respectively after treatment.And the curative effect was evaluated and compared between two groups;The adverse reactions were recorded and analyzed in the period of treatment.Results After treatment,the FBP,2 h BG,24 h uPro,BUN,Scr and β2-MG of patients in two groups were decreased significantly (P ＜ 0.05),and the change of combined group was higher than those control group significantly (P ＜ 0.05).After treatment,the total efficiency of combined group was higher than that patients in control group (P ＜ 0.05).The incidence of adverse reactions of control group in the period of treatment was 20.0％ (10/50),and combined group was 5.8％ (3/52),and which difference from two groups was no significance.Conclusions L-carnitine combined with alprostadil for patients with diabetic nephropathy deserved popularization in clinic,and which not only possessed remarkable curative effect,but also well controlled the blood glucose level,improved the renal function,decreased the incidence of adverse reactions certainly.
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<p><b>AIM</b>To evaluate the hepatocyte protective effect of agarohexaose against indirect oxidative stress injury induced by antimycin A.</p><p><b>METHODS</b>Antimycin A was used to induce oxidative injury of human hepatocyte L-02. The oxidative degree in cells was detected by dichlorofluorescin diacetate (DCFH-DA) and the fluorescence generation was recorded by flow cytometer and fluorescent microscope. The apoptosis of L-02 cells induced by oxidation was identified by TUNEL test, and the morphologic features of cells were also observed.</p><p><b>RESULTS</b>Agarohexaose at concentration of 1 mg x mL(-1) inhibited the oxidation of DCFH into DCF significantly. The fluorescence intensity and oxidized cell number decreased after the incubation with agarohexaose. The photomicrographs of antimycin A and agarohexaose treated cells revealed that agarohexaose could reduce the apoptotic morphologic features. The TUNEL results also indicated that the number of apoptotic cells decreased significantly after the treatment of agarohexaose.</p><p><b>CONCLUSION</b>Agarohexaose could inhibit the sudden increase reactive oxygen species (ROS) in cells significantly, and it also protected cells against oxidative stress injury in vitro.</p>