Study on PI3K inhibitor LY294002 for chemotherapeutic sensitization in diffuse large B cell lymphoma cell lines / 中华血液学杂志

<p><b>OBJECTIVE</b>To investigate the effects on chemotherapeutic sensitization of the PI3K inhibitor LY294002 in diffuse large B cell Lymphoma (DLBCL) cell lines ly1, ly8, ly10.</p><p><b>METHODS</b>The three cell lines were treated with LY294002, or doxorubicin alone or combined or sequentially respectively. Western blotting was used to detect the level of phospho-AKT after the treatment. Flow cytometry combined with annexin V-FITC assay and Brdu incorporation assay were used to analyze the alterations of cell cycle, proliferation, and apoptosis, respectively.</p><p><b>RESULTS</b>LY294002 decreased the level of phospha-AKT efficiently in the three DLBCL cell lines. The ratio of S phase cells was significantly decreased (P < 0.05). Sequential use of LY294002 and doxorubicin increased the ratio of apoptosis and there was significant difference between the sequential group and the other four groups (P < 0.05) at 24, 48, 72(ly1), 48, 72 (ly8) or 24 h (ly10).</p><p><b>CONCLUSION</b>LY294002 can sensitize doxorubicin-induced apoptosis and may be a potential molecular therapeutic agent targeted at AKT signaling pathway in DLBCL.</p>

Effect of hyperlipemia on endothelial function and histomorphology of venous conduit in rabbits before grafting / 第二军医大学学报

Objective: To investigate the effect of hyperlipemia on endothelial function and histomorphology of venous conduit in rabbits before grafting. Methods: Fifty adult male rabbits were randomly divided into 2 groups: one group was fed with normal diet (control group, n=25) and the other with high cholesterol diet (hyperlipemia group, n=25). The blood samples and cervical vein specimens were barvested before and 2, 4, 8 and 12 weeks after feeding. The serum levels of total cholesterol (TC), low density lipoproteins (LDL), triglycerides (TG), and high-density lipoproteins (HDL) were determined. The expression of endothelial NO synthase (eNOS) protein, the production of NO, and the histopathological changes (including the thickness of intima and media, the diameters of the veins, and the presence of lipid or plaque) in the vein specimens were examined. Results: Eight weeks after feeding, the serum levels of TC, LDL, TG, and HDL in hyperlipemia group were significantly higher than those in the control group (P<0.01). Obvious lipid plaques were formed in the carotid artery of rabbits in hyperlipemia group. The jugular veins of hyperlipemia rabbits had significantly lower eNOS expression and NO production (P<0.05). Endothelial denudation was noticed and the elastic fibers almost disappeared in hyperlipemia group; there were no foam cells and lipid plaques. Conclusion: Hyperlipemia may result in endothelium dysfunction and histomorphological change of venous conduit.