RESUMO
Objective To investigate the lignans from the stems of Clausena emarginata. Methods The compounds were isolated and purified by chromatography on kieselguhr, silica gel, MPLC, and preparative HPLC. Their structures were identified on the basis of spectral data and physicochemical properties. Inhibitory activities on LPS-induced NO production of the lignans were initially investigated. Results Sixteen lignans were isolated from the CHCl3 fractions of 95% ethanol extract of the stems of C. emarginata, and their structures were identified as buddlenol C (1), hedyotol D (2), hedyotol C (3), 3-(2,4-dihydroxy-3-methoxybenzyl)-4- (4-hydroxy-3-methoxybenzyl) tetrahydrofuran (4), tripterygiol (5), busaliol (6), 2,3-bis [(4-hydroxy-3,5-dimethoxyphenyl)-methyl]- 1,4-butanediol (7), polystachyol (8), syringaresinol (9), nitidanin (10), 4-[3-hydroxymethyl-5-((E)-3-hydroxypropenyl)-7-methoxy- 2,3-dihydrobenzofuran-2-yl]-2,6-dimethoxy-phenol (11), erythro-guaiacylglycerol-β-O-4’-sinapyl ether (12), erythro-guaiacylglycerol- 8-O-4’-(coniferyl alcohol) ether (13), erythro-1-(4-hydroxy-3-methoxyphenyl)-2-(4-formyl-2-methoxyphenoxy)-propane-1,3-diol (14), rosalaevin B (15), and dehydroconiferyl alcohol (16). Conclusion Compounds 1-15 are isolated from this plant for the first time. Compounds 8 and 16 show inhibitory effects against LPS-induced NO production in microglia BV2 Cell.
RESUMO
The genus Actaea plants are widely distributed in China, and the cycloartane triterpenoids are the characteristic constituents of this genus. They are divided into types of cimigenol, hydroshengmanol, shengmanol, cimiacerogenin, acteol, 16, 23-diketo, foetidonol, dahurinol, etc. Cycloartane triterpenoids show many biological activities, such as cytotoxicity, anti-osteoporosis, antiviral, anti-inflammatory, anti-nucleoside transport, neuroprotective, anti-oxidant, antibacterial activities. The present paper reviewed the distribution of the plant resources of Actaea, chemical structures and biological activities of cycloartane triterpenoids, aiming to provide a reference for the further research in the future.