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Objective To understand the history and current situation of digestive endoscope cleaning and disinfec-tion in domestic medical institutions.Methods Questionnaire survey was used to investigate and analyze the history and current situation of digestive endoscope cleaning and disinfection in medical institutions in some provinces and cities in China.Results A total of 278 medical institutions in 11 provinces and cities of China participated in the in-vestigation,78,175,and 278 institutions filled out complete information in 1986,2005,and 2015 respectively.The personnels participated in cleaning consisted of doctors,nurses and workers,the combination of nurses and workers increased from 2.56% in 1986 to 23.74% in 2015.In 1986,100% of medical institutions used storage water for di-gestive endoscope cleaning;in 2005 and 2015,all used running water for cleaning,besides,30.29% of medical in-stitutions used filtered water or purified water for cleaning,in 2015,50.72% of medical institutions used filtered water,17.63% used purified water for cleaning.In 1986,no cleaning enzyme were used,in 2015,98.92% of med-ical institutions used enzyme for cleaning;in 1986,irrigation of digestive endoscopy channel was performed with simple rinsing(94.87%),in 2015,enhanced irrigation (34.53%)and automatic irrigation (53.24%)were the main rinsing modes.In 2005 and before,>90% of digestive endoscope cleaning and disinfection were not recorded or manually recorded,in 2015,36.33% of institutions used information traceability system.Conclusion The staffing of digestive endoscope cleaning in domestic medical institutions,quality of cleaning water,selection of high efficien-cy disinfectant,monitoring and recording of digestive endoscope cleaning and disinfection in domestic medical insti-tutions were greatly improved compared with 1986 and 2005,cleaning and disinfection gradually standardized,disin-fection and sterilization methods of digestive endoscope tend to be diversified.
RESUMO
Objective To understand the history and current situation of digestive endoscope cleaning and disinfec-tion in domestic medical institutions.Methods Questionnaire survey was used to investigate and analyze the history and current situation of digestive endoscope cleaning and disinfection in medical institutions in some provinces and cities in China.Results A total of 278 medical institutions in 11 provinces and cities of China participated in the in-vestigation,78,175,and 278 institutions filled out complete information in 1986,2005,and 2015 respectively.The personnels participated in cleaning consisted of doctors,nurses and workers,the combination of nurses and workers increased from 2.56% in 1986 to 23.74% in 2015.In 1986,100% of medical institutions used storage water for di-gestive endoscope cleaning;in 2005 and 2015,all used running water for cleaning,besides,30.29% of medical in-stitutions used filtered water or purified water for cleaning,in 2015,50.72% of medical institutions used filtered water,17.63% used purified water for cleaning.In 1986,no cleaning enzyme were used,in 2015,98.92% of med-ical institutions used enzyme for cleaning;in 1986,irrigation of digestive endoscopy channel was performed with simple rinsing(94.87%),in 2015,enhanced irrigation (34.53%)and automatic irrigation (53.24%)were the main rinsing modes.In 2005 and before,>90% of digestive endoscope cleaning and disinfection were not recorded or manually recorded,in 2015,36.33% of institutions used information traceability system.Conclusion The staffing of digestive endoscope cleaning in domestic medical institutions,quality of cleaning water,selection of high efficien-cy disinfectant,monitoring and recording of digestive endoscope cleaning and disinfection in domestic medical insti-tutions were greatly improved compared with 1986 and 2005,cleaning and disinfection gradually standardized,disin-fection and sterilization methods of digestive endoscope tend to be diversified.
RESUMO
<p><b>OBJECTIVE</b>To investigate the effect of RNA interference of the RelB gene on the radiosensitivity of the mouse prostate cancer cell line RM-1 and its mechanism.</p><p><b>METHODS</b>We constructed RelB siRNA-expressing lentiviral vectors targeting the RelB gene with the molecular biological technique, and determined the expressions of RelB mRNA and protein on radiation after transfection with siRelB mediated by liposome using RT-PCR and Western blot, respectively. We also detected the apoptosis of RM-1 cells by FCM assay and their radiosensitivity by clonogenic assay.</p><p><b>RESULTS</b>The expressions of RelB mRNA and protein were significantly lower in the RM-1 cells than in the control and negative interference groups after transfection with RelB siRNA (P < 0.05), while the apoptosis of RM-1 cells remarkably higher in the siRelB-RM-1 than in the control group after radiation treatment (P < 0.05). The activity of MnSOD was markedly decreased (P < 0.05), and the radiosensitization rate of the RM-1 cells in the RelB-RM-1 group was 5.13 after radiation treatment.</p><p><b>CONCLUSION</b>RNA interference of the RelB gene could enhance the radiosensitivity of the mouse prostate cancer cell line RM-1, which might be associated with its inhibition of Mn-SOD expression and induction of cell apoptosis.</p>