Metabolomic characteristics of spontaneously hypertensive rats under chronic stress and the treatment effect of Danzhi Xiaoyao Powder, a traditional Chinese medicine formula / 中西医结合学报

OBJECTIVE@#Numerous studies have demonstrated the close relationship between chronic stress and blood pressure (BP). Hypertensive subjects exhibit exaggerated reactions to stress, especially higher BP. The mechanisms by which stress affects pre-existing hypertension still need to be explored. Danzhi Xiaoyao Powder (DP), a historical traditional Chinese medicine formula, is a promising treatment for BP control in hypertensive patients under stress. The present study investigated the metabolomic disruption caused by chronic stress and the treatment effect and mechanism of DP.@*METHODS@#Spontaneously hypertensive rats (SHRs) were subjected to chronic restraint stress (CRS) for 4 weeks. BP was measured via the tail-cuff method, and anxiety-like behavior was quantified using the elevated-plus-maze test. Meanwhile, DP was administered intragastrically, and its effects were observed. Global metabolomic analysis was performed using ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry, followed by multivariate statistical analysis to detect differential metabolites and pathways.@*RESULTS@#DP alleviated the CRS-induced increase in BP and anxiety-like behavior. Systematic metabolic differences were found among the three study groups. A total of 29 differential plasma metabolites were identified in both positive- and negative-ion modes. These metabolites were involved in triglyceride metabolism, amino acid (phenylalanine, tryptophan, and glycine) metabolism, and steroid hormone pathways.@*CONCLUSION@#These findings expose the metabolomic disturbances induced by chronic stress in SHRs and suggest an innovative treatment for this disorder.

Investigation and Research on the Influencing Factors of Migrant Workers' Participation in Basic Medical Insurance for Urban Employers / 中国卫生经济

Objective:To study the effect of individual and system characteristics on migrant workers' participation in basic medical insurance for urban employers.Methods:The data of "2014 National Floating Population Dynamic Monitoring Survey" and official documents from 28 provincial capital cities and municipalities directly under the central government were used to analyze the influencing factors of 50 679 migrant workers' participation in medical insurance.Results:The characteristics of human capital,economic characteristics,mobility and access to basic public health services of migrant workers all had significant impacts on their participation in medical insurance;local governments' support for trans-provincial transfer medical insurance,the years of payment could be accumulated while one to one conversion and personal account could be roll-in roll-out were important factors to promote migrant workers to participate in medical insurance,and also helped to attract interprovincial migrant workers.Conclusion:In the system design,the overall level of medical insurance should be improved,and the obstacle to identification of the years of payment and the transfer of personal medical insurance account should be cancelled.