Effect of hypoxia on the expression of urokinase-type plasminogen activator in human tongue squamous cell carcinoma cells (Tca8113) / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To observe the expression of urokinase-type plasminogen activator (uPA) in human tongue squamous carcinoma cells (Tca8113) under hypoxia, in order to explore the relation between hypoxia and invasion and metastasis of oral cancer.</p><p><b>METHODS</b>Under different hypoxic times(0, 3, 6, 12, 24 h), the expression of uPA protein was examined quantitatively using immunohistochemical technique (IH) and flow cytometry (FCM).</p><p><b>RESULTS</b>Hypoxia promoted the expression of uPA. The longer hypoxic time was, the higher expression of uPA was.</p><p><b>CONCLUSION</b>Hypoxia can promote invasion and metastasis of oral squamous carcinoma through stimulating the expression of uPA.</p>

Influence of the expression of heat shock protein 70 in maxillofacial squamous cell carcinoma by thermochemotherapy / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To study the influence of thermochemotheraphy on the expression of HSP70 in maxillofacial squamous cell carcinoma.</p><p><b>METHODS</b>12 patients were treated with thermochemotheraphy twice a week, altogether 10 times. After 8 mg of Pingyangmycin infused, the patients were treated with microwave hyperthermia at 43 degrees C for 40 min. The part of carcinoma tissue was removed with surgical operations at before treatment and aftre five times of treatment. The expression of HSP70 in tumor cells was determined by SP immunohistochemcial method.</p><p><b>RESULTS</b>The expression of HSP70 in tumor cells was enhanced obviously by thermochemotherapy.</p><p><b>CONCLUSION</b>Special high expression of HSP70 in the tumor cells was induced by thermochemotherapy. With the antigen presenting action and other action, HSP70 have special antitumor effect.</p>