RESUMO
Two strains of Avian leukosis virus subgroup B (ALV-B) were isolated for the first time in China Hy-line White on the cultured DF-1 cells which were inoculated tissue samples from by an ELISA assay, a histopathology examination and a PCR-based diagnosis. The results from the ELISA assay indicated that the positive rate of serum antibodies to ALV-B and ALV-J virus were 16.3% (15/92) and 13% (12/92), respectively. The histopathological examination indicated that two types of tumor cells existed at same focus in liver and spleen, which mainly were myelocytoma cells and lymphosarcoma cells. The PCR-based diagnosis were performed as follows: the cellular DNA was extracted from the inoculated DF-1 cells; the specific fragments of 1100 bp and 924 bp were obtained by a PCR system with the diagnostic primers of ALV-B and ALV-J; and the PCR results for ALV-A, MDV and REV were all negative. Then, the amplified fragments of the two ALV-B stains were partially sequenced and shown an identity of 92.8%,94.7% with the prototype strain of ALV-B (RSV Schmidt-ruppin B). The identities of two ALV-J strains with the prototype strain HPRS-103 at 96.9%, 91.5%; The identities of two ALV-J strains with the American prototype strain at 85.9%, 81.5%. Our study had shown that ALV-B was isolated for the first time from the ALV-J infected commercial layer flocks in China. It also indicated that the chance of genetic recombination among various subgroups of ALV was increased.
Assuntos
Animais , Leucose Aviária , Patologia , Virologia , Vírus da Leucose Aviária , Classificação , Genética , Linhagem Celular , Galinhas , China , Fígado , Patologia , Virologia , Dados de Sequência Molecular , Filogenia , Doenças das Aves Domésticas , Patologia , Virologia , Baço , Patologia , VirologiaRESUMO
<p><b>OBJECTIVE</b>To comprehend the latest HIV-I epidemic tendency and the character of V3 loop in MSM population of Beijing. METHODS; The C2-V3 regions of the HIV envelop gene were amplified by nest-PCR and sequenced from 11 HIV-l-infected MSM in Beijing in 2007. The subtype and sequences of V3 loop was analyzed. RESULTS There are 4 subtype B strains, 5 CRF AE, 1 CRFO7BC and 1 CRF15-01B strains within all 11 strains. There are five types of central motifs of the 11 samples, in which GPGR and GPGQ are most common.</p><p><b>CONCLUSION</b>Recombination subtype of HIV-1 are spread extensively in MSM population of Beijing.</p>
Assuntos
Adulto , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem , Sequência de Aminoácidos , China , Infecções por HIV , Virologia , HIV-1 , Química , Classificação , Genética , Homossexualidade Masculina , Dados de Sequência Molecular , Filogenia , Alinhamento de Sequência , Produtos do Gene env do Vírus da Imunodeficiência Humana , Química , GenéticaRESUMO
<p><b>OBJECTIVE</b>To investigate alterations of hyper variable region 1 (HR 1) of mitochondrial DNA Blood cells were (mtDNA) in white blood cells of Chinese Han nationality HIV/AIDS patients.</p><p><b>METHODS</b>obtained from 47 cases of therapy-naïve HIV/AIDS patients without opportunity infection and DNA were extracted using blood DNA extracted kit. About 600 bp fragments which contain HR 1 were amplified by PCR. Alterations were determined by directed DNA sequencing.</p><p><b>RESULTS</b>There were 124 polymorphism sites in mtDNA HR 1 (nb16024-16383) in 47 HIV/AIDS patients. The alteration rate was 0 to 20.47% (median 5.33%). 36 cases experienced C to T nucleotide change at nt 16 223, and the alteration rate was 70.97%. At nt 16 362, 26 individuals showed T to C nucleotide change and 3 individuals showed T to G alteration, alteration rate was 55.32% (26/47) and 6.38% (3/47) individually.</p><p><b>CONCLUSION</b>HIV infection may cause more alterations in HR 1 regions.</p>
Assuntos
Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Povo Asiático , Etnologia , Genética , Sequência de Bases , Células Cultivadas , China , DNA Mitocondrial , Química , Genética , Variação Genética , Infecções por HIV , Etnologia , Genética , Leucócitos , Química , Dados de Sequência Molecular , Mutação , Polimorfismo Genético , Homologia de Sequência do Ácido NucleicoRESUMO
<p><b>OBJECTIVE</b>To approach the effect of HAART to serum soluble interleukin-2 receptors in AIDS patients.</p><p><b>METHODS</b>90 cases of AIDS patients were chosen for detection of their slL-2R levels by enzyme-linked immunosorbent assay (ELISA), peripheral blood CD4 T cell counts by flow cytometry and viral load by Branch DNA amplification.</p><p><b>RESULTS</b>The slL-2R levels were (3154 +/- 905) m9icrog/ml before HAART and (2216 +/- 884) pg/ml after( P < 0.001), After HAART, the sIL-2R levels were(2846 +/- 963) microg/ml in ineffective HAART group and (1879 +/- 875) pg/ml in effective HAART group (P < 0.001).</p><p><b>CONCLUSION</b>The serum sIL-2R levels of AIDS patients were lowered by effective HAART, and it could judge the efficacy of HAART in same extent.</p>