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ObjectiveTo predict the target genes of miRNA associated with hsa_circRNA_102802 and hsa_circRNA_104597 using bioinformatics methods,and analyse the biological process and signaling pathway.MethodsTargetscan,miranda and mirbase three online database were used to predict target genes of miRNAs which are complementary to circRNAs.Target genes of miRNAs prediction results were taken their intersection of three online database,then take their collection as total target genes of miRNAs.The results were analyzed by gene ontology(GO) and KEGG pathway analysis using FunNet.Results199 target genes of has_miR_204_5p,has_miR_809,has_miR_520_5p,has_miR_423_5p,has_miR_617 and has_miR_877_5p were gotten from the intersection of 3 databases;and 410 targets of has_miR_659_3p,has_miR_9_5p,has_miR_661,has_miR_576_3p,has_miR_548d_5p,has_miR_548a_5p,has_miR_548b_5p,has_miR_876_5p and has_miR_744_5p were gotten from the intersection of 3 databases.GO analysis showed that Target genes involved in diverse biological processes including central nervous system,such as cortex development,axon guidance and extension,synaptic transmission,as well as the process of learning and memory (P<0.05).Enriched pathways were also revealed target genes involved in the signal path including the axon guidance,glutamic acid synapses,Wnt signaling pathway,ErbB signaling pathway,mTOR signaling pathway and VEGF signaling pathway,which connect closeiy with depression.hsa_circRNA_102802 and hsa_circRNA_104597 may play an important role in depression.Conclusionshsa_circRNA_102802 and hsa_circRNA_104597 may be associated with the pathologenesis of depression disorder.
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Objective To investigate the effects of amino acid receptor antagonists in the treatment of vascular dementia after cerebral apoplexy in elder in the function of study and memory.Methods 68 elder patients(≥65 years older) with vascular dementia caused by stroke from August 2014 to December 2015 in tongde hospital of zhejiang province were selected and randomly divided into study group and control group , 34 cases in each group.The control group received the convention treatment and combined with compound haishe capsule three pills each time , three times/day,the study group received convention treatment and combined with memantine three weeks before 5-15 mg/day,fourth week 20 mg/day,two groups were treated for six months.Cerebrospinal fluid,hematological and clinical indexes were compared before and after treatment.Results Compared with before treatment, levels of SS and AVP of cerebrospinal fluid in two groups after treatment increased,levels of MMSE and HDS-R increased,scores of ADL decreased, levels of P300 in latency decreased and levels of P300 in amplitude increased,levels of VEGF and BDNF increased,the differences were statistically significant (P<0.05).Compared with the control group,levels of SS and AVP in cerebrospinal fluid in the study group were higher,levels of MMSE and HDS-R were higher,scores of ADL were lower,levels of P300 in latency were lower and levels of P300 in amplitude were higher,and levels of VEGF and BDNF were higher,the differences were statistically significant (P<0.05).Conclusion Amino acid receptor antagonists can increase the ability of learning and memory in patients with senile vascular dementia after stroke, and it can improve the cerebrospinal fluid and blood indexes.
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Objective To explore correlation between alexithymia and resilience in military personnel.Methods 2802 subjects were chosen by random cluster sampling to outline the military personnel resilience scale and military alexithymia scale.The data were analyzed by t-test,chi-square test,ANOVA,correlation analysis and multiple linear regressions analysis.Results The prevalence rate of alexithymia was 6.85％,while resilience was 6.60％.Compared to sea force (45.62 ± 11.25 ) and air force (45.32 ± 9.98 ) subjects,land force (47.92 ± 9.92)had significantly higher total score in alexithymia(P＜ 0.05 ).Compared to sea force (87.35 ± 12.15 ) and air force ( 88.58 ± 10.39 ) subjects,land force ( 85.73 ± 10.70) had significantly lower total score in resilience(P ＜ 0.05 ).The total score and every factor score of subjects with alexithymia were significant lower than those of control group in resilience(P ＜ 0.05 ).Every factor of Resilience Scale were mild negative correlation with every factor of Alexithymia Scale (P＜0.01).Problem solving,volition,optimism and family supports were the main factors which had affected on the alexithymia.Conclusion The prevalence rate of alexithymia in military personnel is lower than ordinary people,but there are still issues in alexithymia and resilience,particularly in land force.The research show mild correlation between alexithymia and resilience in military personnel.