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Objective To investigate the relationships between expression of FAK and Prostate Carcinoma (PC)morbidity. Methods By surgery,get cancer tissue and para-carcinama tissue from 60 cases PC.To detect FAK expression by immuno-histochemical.To extract total RNA by Trizol.To detect the FAK mRNA by RT-PCR,and analysis these data.Results FAK expression level in cancer tissue was higher than that in para-carcinama tissue.There was statistical difference between them (χ2=72.55,P<0.01).mRNA expression levels of FAK in cancer tissue showed significant higher than the levels in para-carcinama tissue (t=30.51,P<0.01).According to lymphatic metastasis,the expression positive cases of FAK in pN0M0 classification were lower than these in pN3M1 classification,and mRNA expression levels of FAK were the same re-sults.There were clearly statistical distinctive (t=25.43,P<0.01).Conclusion The expression of FAK in PC cells was as-sociated with tumor invasion.
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Objective To study the phenotype and function of CD4 +CD25 + Treg cells in the peripheral blood of patients with systemic sclerosis (SSc) and their relationship with fibrosis.Methods The proportion of Foxp3, CD127, CTLA-4 and CD69 on CD4+CD25+ Treg cells in peripheral blood were detect by flow cytometry;the levels of TGF-[1 and IL-10 in serum were detect by enzyme-linked immunosorbent assay (ELISA) in patients with SSc.The correlation between Treg cells and the score of chest HRCT, MRSS, and disease activity was analyzed.T test and Pearson correlation analysis were used for statistical analysis.Results ① Compare to the control group, the proportion of CD4+CD25+ Treg cells in peripheral blood of SSc patients was increased significantly(12.9±2.4 vs 14.9±2.2, t=2.63, P=-0.012), and the expression of CD69+, CTLA-4+ on CD4+CD25+ Treg cells was decreased significantly (P＜0.01).② Compare to the control group, the proportion of CD4+CD25+Foxp3 + cells and CD4+CD25+CDI27-cells in peripheral blood of SSc patients was increased significantly (respectively, 3.3±0.7 vs 5.0±0.7, 5.1±1.6 vs 7.6±2.0, t=7.03, 4.195;P＜0.01), but no correlation between them was detected.③ The level of TGF-β1 in the serum of the SSc patients was lower than that of the control group(86±29 vs 133±29 ng/ml, t=-5.026, P=0.000).However, IL-10 had no significant difference between the two groups.④ The proportion of CD4+CD25 +Foxp3 + cells and CD4~D25 +CD127-cells in peripheral blood of SSc patients was positively correlated with the scores of chest HRCT (respectively, r=-0.541, P=0.02;r=0.486, P=0.041), and no correlation was observed with ESR, CRP.In addition, CD4+CD25+Foxp3+ cells were associated with MRSS.Conclusion The proportion of CD4+CD25+ Treg cells in the peripheral blood of SSc patients is increased, but they alters the immune function.The different phenotypes of Treg cells of CD4+CD25+Foxp3+ cells and CD4+CD25+CD127-cells in peripheral blood of SSc patients are increased significantly, which changes along with skin and lung fibrosis.The associated cytokine TGF-β1 is reduced, and IL-10 is not significantly changed.
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Objective To investigate the pathological features, distinguishment and diagnosis and prognosis of multiple cystic renal cell carcinoma. Methods Six patients with multilocular cystic renal cell carcinoma in our hospital from 2007 to 2010 were chosen and reviewed for analysis. All patients who were found to have mass after physical examina-tion, came to our hospital, and image examination was conducted, and pathological detection and immunohistochemical detection were conducted after surgical resection. All the results were recorded. After hospitalization, all patients were followed up for a period of five years, and prognosis was recorded. Results Among 6 cases of multilocular cystic renal cell carcinoma patients, there were three cases of male patients and three cases of female patients. Pathological exami-nation revealed the apparent limits of diseased region and normal part, the surface coating, no vascular invasion, and no metastasis. The average tumor diameter was (3.52士0.48) cm, and different cysts of varying sizes were visible in section, with visible bloody or serous liquid in cavities. Microscopic examination showed clear cell of monolayers lining in cysts, partially with papillary structures, and there were inflammatory cells in interval composed of fibrous tissue. Tumor cells included translucent pulp and stained chromatin. According to fuhrman nuclear grade, 5 patients were grade Ⅰ, and 1 patients was gradeⅡ. Immunohistochemical detections such as Vim and EMA were strongly positive, and CD68 and CD10 were negative. Six patients had no recurrence within 5 years, and 5-year survival rate was 100%, with good prog-nosis. It should be noted that multi-cystic renal cell carcinoma and other renal cystic diseases should be identified. Conclusion Pathological features of multi-cystic renal cell carcinoma are unique, and we should pay attention to iden-tification of multi-cystic renal cell carcinoma and other renal cystic diseases. The prognosis of multi-cystic renal cell carcinoma is good.
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OBJECTIVE@#To evaluate the capability and limitation of magnetic resonance image(MRI)for Lumbar zygapophyseal joint cartilage through comparing pig lumbar zygapophyseal joint cartilage acquired from multiple MRI sequences of a 1.5 Tesla MR and gross specimens.@*METHODS@#Six fresh lumbar spines from adult pigs were sagittaly scanned by Siemens 1.5 Tesla MR. The scan sequences included fast spin echo T1-weighted imaging (FSE T1WI), fast spin echo T2-weighted imaging (FSE T2 weighted T2WI), fat saturation proton density-weighted imaging (FS PDWI), 3-dimensional fast low angle shot imaging (3D-FLASH), and water excitation 3-dimensional fast low angle shot imaging (WE 3D-FLASH). Each scan sequence acquired images from the same layer. The signal-noise ratio (SNR) for articular cartilage, contrast-noise ratio (CNR) for cartilage versus bone cortex, cartilage versus bone marrow, and cartilage versus saline were calculated. Right after the scanning, the lumbar spines were snap-frozen, incised sagittally along the midline lumbar zypapophyseal joints, and photographed to compare the gross specimens with corresponding MRIs. The thickness of sagittal midline center of 6 pairs of lumbar(L₃/L₄) zypapophyseal joint cartilage was measured by vernier caliper. The thickness of the back ventral articular cartilage was added and then compared with corresponding MR images.@*RESULTS@#3D-FLASH (FA 20°) and WE 3D-FLASH (FA 20°) sequences had significant advantages compared with other sequences in imaging lumbar zypapophyseal joint cartilage, and were mostly close to the real thickness.(1) Comparison of the 4 flip angle (FA 10°, FA 20°, FA 30°, and FA 40°) 3D-FLASH sequences:The highest cartilage SNR and best CNR of cartilage versus bone cortex were both found in the 3D-FLASH(FA 20°) sequence, which was significantly different from the other three 3D-FLASH sequences.The satisfactory CNR of cartilage versus bone marrow, cartilage versus saline were found more in the 3D-FLASH(FA 20°) sequence. (2) Comparison of the 4 flip angle(FA 10°, FA 20°, FA 30°, and FA 40°) WE 3D-FLASH sequences: the highest cartilage SNR,best CNR of cartilage versus bone cortex,and best CNR of cartilage versus bone marrow were found in the WE 3D-FLASH (FA 20°) sequence, which was significantly different from the other three 3D-FLASH sequences. The CNR of cartilage versus saline was found more satisfactory in the WE 3D-FLASH (FA 20°) sequence. (3) The highest cartilage SNR and best CNR of cartilage versus bone cortex were both found in the 3D-FLASH (FA 20°) sequence, which was significantly different from those in the PDWI, FSE T1WI,and FSE T2WI sequences (P0.05) in the WE 3D-FLASH (FA 20°) sequence. The highest CNR of cartilage versus bone marrow was seen in WE 3D-FLASH (FA 20°) sequence. It was statistically significant compared with that in FS PDWI,FSE T1WI, and T2WI sequences respectively, but the difference was not significant compared with 3D-FLASH (FA 20°) sequence (P>0.05). Both the FS PDWI and T2WI sequences displayed ideal CNR of cartilage versus saline, with no significant difference (P>0.05). The lower SNR of cartilage versus saline was shown in 3D-FLASH (FA 20°) and WE 3D-FLASH (FA 20°) sequence, and the difference was not significant (P>0.05). However, they were significantly different compared with FS PDWI and T2WI sequences (P<0.05). (4) WE 3D-FLASH (FA 20°) and 3D-FLASH (FA 20°) sequences were relatively better than the FS PDWI when comparing the thickness of articular cartilage, which was significantly different from the FS PDWI sequence (P<0.05).@*CONCLUSION@#The 3D-FLASH sequence and derived WE 3D-FLASH sequence have better definition of cartilage images and are mostly close to the real thickness, which possibly are the optimal scanning sequences for lumbar zypapophyseal joint articular cartilage MR imaging.
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Animais , Cartilagem Articular , Imageamento Tridimensional , Vértebras Lombares , Imageamento por Ressonância Magnética , Métodos , Suínos , Articulação ZigapofisáriaRESUMO
OBJECTIVE To understand the related factors,the situation and dynamics of hospital infection in order to effectively prevent and control it.METHODS From 12306 cases of inpatients who had investigated retrospectively,706 cases were with nosocomial infection monitored from Jan to Dec 2006.RESULTS The hospital infection rate was 5.74% in 2006.The hematological disease and,cancer patients and the elderly,were the high-risk population.The main infection site was the respiratory tract.Fungi accounted for 21.35%,the top was the Candida albicans.CONCLUSIONS To strengthen the training of medical staff,to monitor the key departments of hematology and cancer and respiratory diseases and to attend the disease surveillance and rationally use of antibiotics are the effective measures to reduce hospital-acquired infections.
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Based on the characteristics of medicinal chemistry and the training objectives of experimental teaching,to explore the teaching reform of comprehensive experiments in medicinal chemistry.This paper discussed the time of biginning comprehensive experiments,the choice of experimental article,the demands on teachers in comprehensive experimental teaching,and attention points of teaching management.