Correction to: Increasing targeting scope of adenosine base editors in mouse and rat embryos through fusion of TadA deaminase with Cas9 variants

In the original publication the grant number is incorrectly published. The correct grant number should be read as "17140901600". The corrected contents are provided in this correction article. This work was partially supported by grants from the National Natural Science Foundation of China (Nos. 81670470 and 81600149), a grant from the Shanghai Municipal Commission for Science and Technology (17140901600, 18411953500 and 15JC1400201) and a grant from National Key Research and Development Program (2016YFC0905100).

Prospective value of early postoperative PI-APD in children with ureteropelvic junction obstruction / 上海交通大学学报（医学版）

Objective· To investigate the prospective value of early postoperative PI-APD in children with hydronephrosis.Methods· Data of children with hydronephrosis who underwent pyeloplasty in Xinhua Hospital,Shanghai Jiao Tong University School of Medicine between Jan 2012 to Nov 2015 was collected.PI-APD was divided into 3 categories (≤ 19％,19％＜PI-APD＜40％ and ≥ 40％).The relationship between PI-APD value and the degree of renal function (DRF) and dilation recovery after surgery was analyzed.Results· There were 360 children with hydronephrosis.The median follow-up was 20 months.The PI-APD value (3 months after pyeloplasty) was positively correlated with the degree of DRF recovery (r=0.631,P=0.000).Five patients received redo-pyeloplasty.PI-APD of all these patients was ＜19％.Conclusion· PI-APD is a new feasible ultrasound parameter in pyeloplasty followup.PI-APD ≥ 40％ at the first post-operative visit predicts pyeloplasty success.PI-APD ≤ 19％ indicates close follow-up after operation.PI-APD can also help select children at high risk for repeat intervention after pyeloplasty.

The culture and identification of urinary tract smooth muscle cells / 中华泌尿外科杂志

Objective To establish a method of isolating,culturing,proliferating urinary tract(nephropelvics,ureter and bladder)smooth muscle cells (SMC). Methods Urinary tract SMC were isolated from sample tissues (1 from human nephropelvics,1 from human ureter,3 from human bladders,5 from porcine bladders and 1 from porcine ureter) by collagenase digestion and were cultured in DMEM supplemented with fetal bovine serum.Morphology and expansion of the cells were observed.SMC specific protein (?-actin) was identified by immunohistochemical methods. Results The cells grew well and presented a typical morphological feature of SMC.They could be passaged 6 times without a remarkable decrease in rate of cell proliferation.Immunohistochemical staining showed that ?-actin was positive. Conclusions This urinary tract SMC cultural method was simple,reliable and fruitful.