Correlation between serum level of miRNA-106a expression with clinicopathological characteristics and prognosis of patients with renal cell carcinoma / 中华医学遗传学杂志

OBJECTIVE@#To analyze the expression of microRNA-106a(miR-106a) in renal cell carcinoma (RCC) and its correlation with clinicopathological characteristics and prognosis of patients.@*METHODS@#Serum samples of 64 patients with newly diagnosed RCC were collected as the study group, and serum samples of 40 healthy individuals were used as the control group. Real-time fluorescence quantitative PCR was used to determine the expression level of miR-106a in each group. The correlation between miR-106a expression and clinicopathological characteristics of the patients was studied with single factor analysis and multiple Logistic regression model. Kaplan-Meier survival curve was used to analyze its correlation with the prognosis of patients.@*RESULTS@#Before surgery, compared with the control group (1.17± 0.58), RCC patients with high- (9.15± 0.96) and low-expression(3.45± 0.37) had increased expression of miR-106a. Postoperatively, the expression level of miR-106a in both groups of patients decreased to 1.53± 0.18 and 1.75± 0.21, respectively. The area under the curve (AUC) of the diagnostic value of serum miR-106a for RCC was 0.782 (95% CI: 0.661-0.902). With an optimal cutoff value of 0.531, the sensitivity was 78.10% and the specificity was 75.00%. Serum miR-106a level of RCC patients with TNM stage T3 or T4, clinical stage II or III, lymph node metastasis, and recurrence were significantly increased. The high expression of serum miR-106a in RCC patients has an independent relationship with the tumor TNM stage and lymph node metastasis. Of the 64 follow-up patients, 4 were lost and 30 had died. Among them, the median survival time of patients in the miR-106a high expression group was 30 months, which was significantly shorter than that of the low expression group (52 months).@*CONCLUSION@#The serum level of miR-106a is elevated in RCC patients, and may be used as a molecular marker for the diagnosis of RCC. High serum expression of miR-106a is an independent predictor for tumor TNM stage and lymph node metastasis, as well as an independent predictor for poor prognosis of RCC patients.

Study on Fragmentation Pathways of Amoxicillin by Electrospray Ionization Multi-stage Tandem Mass Spectrometry / 中国药房

OBJECTIVE: To investigate fragmentation pathways of amoxicillin using electrospray ionization multi-stage tandem mass spectrometry,and to provide reference for quality and quantity analysis of amoxicillin.METHODS: Amoxicillin was further purified by high performance liquid chromatography,and the fragmentation mechanisms were investigated by electrospray ionization multi-stage tandem mass spectrometer.RESULTS: The ?-lactam ring of amoxicillin has three different opening modes;2 kinds of different m/z:114 ion fragments are obtained by two different fragmentation pathways;m/z:349 ion fragments can be a characteristic fragment of amoxicillin for quality and quantity analysis of amoxicillin.CONCLUSION: The method is sensitive and accurate for further study of transformation and metabolism of amoxicillin and its quality and quantity analysis.