Impact of the Varian real-time position management respiratory gating system on radiotherapy planning dosimetry / 中华放射医学与防护杂志

Objective:To study the impact of the Varian real-time position management (RPM) respiratory gating system on radiotherapy planning dosimetry.Methods:The radiotherapy plans of 40 cases with thoracic or abdominal tumors were retrospectively selected in this study. The motion phantom for quality control was adopted to generate respiratory gating signals, and the 30%-60% stable phase at the end of expiratory was selected as the respiratory gating window. The dose verification for the abovementioned radiotherapy plans was performed using the Portal Dosimetry (PD) system under RPM respiratory gating mode with the Edge accelerator. Afterwards, dose analysis was performed with different γ passing rate criteria and the distribution characteristics of γ values were analyzed. Finally, the verification results between the non-gating mode and the gating mode were compared.Results:Under the respiratory gating mode, the passing rates of all intensity-modulated radiation therapy/volumetric-modulated arc therapy (IMRT/VMAT) plans with or without flattening filters were over 95.5% by γ criteria of (3%, 3 mm) or (3%, 2 mm) and were over 90% by stricter γ criteria of (2%, 2 mm). All plans met the clinical requirements recommended by the American Association of Physicists in Medicine (AAPM). The passing rates of dose verification under non-gating mode were slightly better than those under respiratory gating mode, and the differences between the two modes were statistically significant (3%/3 mm, Z =-1.45; 3%/2 mm, Z =-2.86; 2%/2 mm, Z =-3.70; 1%/1 mm, Z =-4.52; P<0.05). There was no significant difference in the minimum and maximum values of γ and the share of γ > 1.5 of plan verification result under the two modes. However, the average value and standard deviation of the γ were generally smaller under the non-gating mode. Conclusions:The impact of the introduction of RPM respiratory gating technology on dose is clinically acceptable, and the execution of these plans in this gating mode is safe and reliable.

LncRNA LINC00313 Knockdown InhibitsTumorigenesis and Metastasis in HumanOsteosarcoma by Upregulating FOSL2through Sponging miR-342-3p

Purpose@#Osteosarcoma (OS) is the most common primary bone tumor, with high morbidity in infants and adolescents. Long noncodingRNA LINC00313 has been found to modulate papillary thyroid cancer tumorigenesis and to be dysregulate in lung cancer.However, the role of LINC00313 in OS has not yet been addressed. @*Materials and Methods@#We evaluated mRNA and protein expression using real-time quantitative PCR and Western blotting. Cellproliferation was evaluated using MTT; apoptosis and autophagy were assessed with flow cytometry, Western blotting, and/orGFP-LC3 assay. Transwell assay was conducted to measure cell migration and invasion. Potential target sites for LINC00313 andmiR-342-3p were predicted with starBase v.2.0 and TargetScan Human, and verified using luciferase reporter assay, RNA immunoprecipitation,and RNA pull-down assay. In vivo, xenogeneic tumors were induced with U2OS and MG-63 cells, separately. @*Results@#LINC00313 was upregulated and miR-342-3p was downregulated in OS tissues and cells. High expression of LINC00313was associated with shorter overall survival. FOSL2 downregulation and miR-342-3p overexpression suppressed cell proliferationand migratory and invasive abilities while promoting apoptosis and autophagy, all of which were consistent with the effects ofLINC00313 knockdown. miR-342-3p, sponged by LINC00313, inversely modulated FOSL2 by targeting MG-63 cells, and FOSL2expression was positively controlled by LINC00313. LINC00313 knockdown suppressed tumor growth in vivo. @*Conclusion@#LINC00313 is upregulated in OS, and LINC00313 knockdown plays a vital anti-tumor role in OS cell progressionthrough a miR-342-3p/FOSL2 axis. Our study suggests that LINC00313 may be a novel, promising biomarker for diagnosis andprognosis of OS.

Risk assessment of different grades of hypertension during the treatment of patients with acute pulpitis / 北京大学学报(医学版)

Objective:To evaluate the vital signs changes,influence factors in different grades of hy-pertension patients during the treatment of acute pulpitis,in order to obtain the risk prevention measures. Methods:In this study,90 different grades of hypertension patients with acute pulpitis were recruited from February 201 4 to February 201 5 in the Department of Oral Emergency,Peking University School and Hospital of Stomatology.The information about the patients’general health,oral treatment,life signs of change information was collected.Patients were divided into high risk group,middle risk group, and low risk group (30 patients for each group).Results:(1 )Compared with the preoperative,systolic blood pressure (90%),diastolic blood pressure (80%),heart rate increase (1 00%)were increased in the high risk group.The increase rates of the middle risk group and the low risk group were significantly lower than those of the high risk group (P<0.01 ).At the same time,the systolic blood pressure of 1 /4 (26.7%)patients in high risk group increased more than 20 mmHg (1 mmHg=0.1 33 kPa),and the diastolic blood pressure of 2/5 patients in high risk group increased more than 1 0 mmHg,the difference was statistically significant compared with the other two groups (P<0.05).(2)Compared with the pre-operative,the average increase of the maximum peak were increased [systolic blood pressure (1 8.0 ± 1 .5)mmHg,diastolic blood pressure (8.0 ±1 .7)mmHg],the mean of heart rate changes [(7.0 ± 0.3)beats per minute]was also increased in the high risk group,while these two indicators were de-creased in the low risk group and the middle risk group.The electrocardiogram (ECG)was changed in 6 cases during the treatment in the high risk group.No significantly changed were observed in the low risk group and the middle risk group.(3 ) Compared the risk assessment in preoperative with that in postoperative,in the middle risk group,23 cases were evaluated as medium risk in final evaluation,6 as low risk,and 1 as high risk (risk assessment increased);in the high risk group,20 cases were evaluated as high risk,7 as very high risk,and 3 as medium risk (risk assessment decreased).Conclusion:Oral treatment is very safe for patients with hypertension,but the risk factor,target organ damage,and com-plications will also increase the risk of cardiovascular events in elderly patients during the acute pulpitis treatment.Dentist should take some measures to avoid the risks.

The study of the influence of space teaching on junior-college nursing students; autonomous learning ability / 中国实用护理杂志

Objective We aimed to assess the influence of space teaching on junior-college nursing students' autonomous learning ability.Methods Using random number table,two classes of junior-college nursing students of Grade 2012 were selected and divided into the experimental group (46 students) and the control group (47 students) according to their classes.Students in the control group received routine multimedia teaching.Students in the experimental group received space teaching based on the platform of World University City.The effect of space teaching on developing nursing students' autonomous learning ability was assessed with the help of their test results and the nursing students' autonomous learning ability evaluation form.Results After carrying out space teaching,the average grade,theoretical performance,total points,the total score of autonomous learning ability and the score of each dimension in the experimental group and the differences were remarkably higher than those of the control group (t value was 2.80,2.45,2.29 and 11.58).Conclusions Space teaching can assist in arousing nursing students' learning motivation and improving nursing students' independent learning ability.It can be used for reference and promoted.

Clinical analysis for systemic complications of patients in the dental emergency room / 北京大学学报(医学版)

Objective:To analyze the clinical features of oral emergency patients in general health, and to guide the oral emergency patients in the treatment, and prevention of accidents.Methods:The retro-spective study was performed on the day visits to the emergency department in Peking University School and Hospital of Stomatology from February to November 2013.The basic information of the newly diag-nosed adult, their overall health status, American society of anesthesiologists(ASA) classification and oral diagnosis information were analyzed and summarized.Results:The cardiovascular and cerebrovascular diseases(6.4%), hypertension(13.4%), endocrine system diseases(4.2%)were most common in the patients.With the increase of age, the associated systemic diseases had increased in number and in ASA classification.Periapical disease ( 24.6%) , dental pulp disease ( 24.4%) , and periodontal disease (18.0%) accounted for about 2/3.Conclusion:The general health status of patients with oral emergen-cy worsened with the increase of age.Oral surgeons should make risk assessment before treatment.ASA classification system can help doctors predict the patient risk, take corresponding measures in advance, and help to reduce the occurrence of accidents.

Analysis of frequent species and antibacterial resistance of pathogenic bacteria causing infections in burn patients in a hospital from 2012 to 2014 / 国际检验医学杂志

Objective To investigate the frequent species of pathogenic bacteria causing infections in burn patients and their re‐sistance to commonly used antibacterial agents ,so as to provide references for rational use of antibacterials in clinic .Methods The distribution and drug susceptibility of pathogenic bacteria isolated from secretions of wound surfaces of 140 cases of burn patients from January 2012 to December 2014 were retrospectively analyzed .Results A total of 152 strains of pathogenic bacteria were iso‐lated .The gram‐negative bacteria accounted for 59 .2% ,in which Pseudomonas aeruginosa ,Proteus mirabilis and Acinetobacter bau‐mannii were the most common isolates ;the gram‐positive bacteria accounted for 34 .2% ,in which Staphylococcus aures ,Staphylo‐coccus haemolyticus and Enterococcus faecalis were the most common isolates ;and fungi were accounted for 6 .6% .A majority of these isolates were multiple resistant to the antibacterial agents .Conclusion Culturing ,identifing and carring out drug‐sensitivity test of pathogenic bacteria isolated from burn patients could provide basis for rational application of antibacterial agents and effec‐tive control of infection .

Efficacy of Lanthanum carbonate in maintenance hemodialysis patients with hyperphosphatemia and high calcium times phosphate product / 中国综合临床

Objective To assess the efficacy and adverse reaction of Lanthanum carbonate in patients with hyperphosphatemia and high calcium times phosphate product receiving maintenance hemodialysis.Methods Twenty-three hemodialysis patients with serum phosphate ≥ 2.0 mmol/L and/or the serum calcium times phosphate product ≥ 4.52 mmol2/L2 were prescribed Lanthanum carbonate chewed with meals for 4 weeks.Blood (Hb), serum albumin (ALB), alanine amino shift enzyme (ALT), aspartic acid transaminase (AST) ,calcium(CA), phosphorus (P) calculated the product of calcium and phosphorus (Ca×P) and intact parathyroid hormone (iPTH) level were checked before and after Lantharum carbonate therapy.The adverse reactions were recorded concomitantly.Results Serum phosphate and the serum calcium time phosphate product significantly decreased after 1 month Lanthanum carbonate therapy((2.48±0.55) mmol/L vs.(1.83 ±0.37) mmol/L, (5.21 ± 1.35) mmol2/L2 vs.(4.10±0.96) mmol2/L2;t =2.742,2.936;P＜0.05).Serum calcium, iPTH level and blood cells count remained no change(P＞0.05), while serum ALT, AST concentration within normal range.Five patients complained affordable abdomen discomfort or upset.Conclusion Lantharum carbonate show to effectively and quickly control serum phosphorus levels and calcium time phosphate product in hemodialysis patients without inducing changes in serum calcium and should be considered an ideal phosphate binder.

The rheology properties of common hydrophilic gel excipients / 药学学报

To investigate theological properties of common hydrophilic gel excipients such as Carbopol based on viscosity, the viscosity was determined by rotation method and falling-ball method. Linear regression was made between ln(eta) and concentration, the slope of which was used to explore the relation between viscosity and concentration of different excipients. The viscosity flow active energy (E(eta)) was calculated according to Arrhenius equation and was used to investigate the relation between viscosity and temperature of different excipients. The results showed that viscosities measured by two methods were consistent. Concentration of guargum (GG) and hydroxypropylmethyl cellulose (HPMC) solution had a great influence on the viscosity, k > 5; while concentration of polyvinylpyrrolidone-K30 (PVP-K30) and polyethylene glycol 6000 (PEG6000) exerted a less effect on viscosity, k < 0.2; viscosity flow active energy of different excipients were close, which ranged from 30 to 40 kJ x mol(-1). Therefore, theological properties study could provide the basis for application of excipients and establish a foundation for the research of relation between excipients structure, property and function.

Association between left ventricular hypertrophy and peritoneal transport properties in peritoneal dialysis patients / 中国综合临床

Objective To investigate the association between left ventricular hypertrophy and peritoneal transport properties in Peritoneal Dialysis patients.Methods Sixty-nine Continuous Ambulatory Peritoneal Dialysis (CAPD)patients were enrolled in current study.All patients underwent echocardiography for left ventricular mass index (LVMI).Transport status was categorized as high transport,high average transport,low average transport and low transport based on modified peritoneal equilibration test (PET).The data collected included hemoglobin,albumin,blood urea nitrogen,creatinine,urea clearance (Kt/V) and creatinine clearance rate (Ccr),dialysis vintage,systolic blood pressure,diastolic blood pressure.Results Patients with high transport status were 41 cases,more than those with low transport status(28 cases).The dialysis age of high transport,high average transport,low average transport and low transport vintage were (39.2 ± 21.8),(26.6 ±15.6),(28.6 ± 14.4),(45.7 ± 35.0) years old respectively,and the difference was significant (F =4.128,P ＜ 0.05).The dialysis age in the higher transport group was longer than that in high average transport group and low average transport group (P ＜ 0.05).LVMI has significant positive correlations with D/Pcr at 4th hour,SBP and DBP (r =0.339,0.351,0.316,P ＜ 0.01) and the negative correlation with albmin (r =-0.292,P ＜0.05).Left ventricular hypertrophy(LVH) in all patients was 63.8％,ant it was higher in high transport group than that low average transport and low transport group (x2 =5.455,5.091,P ＜ 0.05) Conclusion High transport status is the most common in CAPD patients.There is high incident rate of LVH in this population.LVH has significant positive correlations with D/Pcr,higher SBP,DBP,and lower albumin.

Clinical validation of detection results of three different blood routine analyzers / 国际检验医学杂志

Objective To evaluate whether the comparability of 3 automatic blood cell analyzers meet the clinical requirements by conducting the comparative study on the detection results of these instruments.Methods With the Sysmex 2100 automatic blood cell analyzer as the reference instrument,Sysmex 1000i and Abbott 1800 as the experimental instrument,the original quality control provided by the instrument factory and the patient′s fresh anticoagulant blood samples in the laboratory were adopted to monitor for continuous 40 d by these three instruments and the detection results of WBC,RBC,HGB,HCT and PLT were analyzed.Results The detection results of these 3 instruments were statistically tested by the F test,the differences showed no statistical significance (P >0.05)and the bias was in 1/2 of the maximum permissible error range in America department clinical test revised regulations (CLIA′88).Conclusion The detection results by these 3 instruments are comparable and can meet the clinical requirements.

Effect of Chinese herbal drug-containing serum of Huoxue Bushen prescription on the proliferation of human umbilical vein endothelial cells and on expression of endothelial nitric oxide synthase through p42/44MAPK pathway / 中华老年医学杂志

Objective To observe the drug-containing Huoxue Bushen (HXBS) serum on the proliferation of human umbilical vein endothelial cells (HUVECs) and p42/44MAPK pathways by using human umbilical vein endothelial cells (HUVECs).Methods The proliterative effects of 20％,10％,5％ drug-containing HXBS serum on HUVECs were observed by MTT.Nitric oxide synthase (NOS) activity was detected by endothelial nitric oxide synthase (eNOS) activity kit.The content of nitric oxide (NO) was determined by NO kit.The mRNA expression of eNOS was detected by real-time reverse transcription polymerase chain reaction (RT-PCR).The protein expression of pP42/44 was detected by Western blotting.Results HUVECs proliferation was effectively promoted by drug-containing HXBS serum.The proliferation rate of HUVECs was increased along with the drug-containing HXBS serum concentration increasing after 24 h treatment (10.8 ％,14.7 ％,33.3 ％,t=6.82,6.25,12.16,respectively,P＜0.05 or 0.01).eNOS activity was significantly increased in the drug-containing HXBS serum-treated group as compared with the control group.There was a significant difference in NO content between 20％ drug-containing HXBS serum treated group and control group (P＜0.05).The expression of eNOs mRNA was significantly promoted in 20％ and 10％ drug-containing HXBS serum groups (P ＜ 0.05).P-p42/44 protein level was significantly increased by drug-containing HXBS serum (P ＜ 0.01).Conclusions Conclusions HXBS can promote endothelial cell proliferation and improve endothelial cell NO secretion by increasing the activity and mRNA expression of eNOS through p42/44 signaling pathway,which provides a theoretical basis for improving cardiovascular disease in postmenopausal women.

Application of comprehensive experiments on multiple trauma emergency rescue in intensive training before nursing practice / 中国实用护理杂志

Objective To evaluate the effect of comprehensive experiments on multiple trauma emergency rescue in intensive training before nursing practice. Methods 772 students were divided into groups, 16 to 18 students in each group,each team distributed teaching notes, designed experiment scheme toget her, selected and played roles, undertook tasks, practiced independently before the class,then performed comprehensive training in class. Results 94.8% of the nursing students and 100.0% of the training teachers supported this method of comprehensive experiments in intensive training. Conclusions Nursing comprehensive experiments can shorten the gap between the clinical practice and class teaching, improve the students' professional ability and general quality. It is a popular education mode on intensive training before clinical practice.

Expression and clinical significance of EGFR and Her-2 in gastric cancer / 肿瘤研究与临床

Objective To analyze the relationship between the survival and expressions of EGFR and Her-2, and explore the prognostic significance of EGFR and Her-2 in gastric cancer patient by detecting the expressions of EGFR and Her-2 in gastric cancer specimens. Methods EGFR and Her-2 were detected by immunohistochemistry. The relation between their expressions with their clinical characteristics and prognosis were analyzed. Results The over-expression rates of EGFR and Her-2 are 27.4 % and 32.1% respectively in 84 cases. The over-expression rates of EGFR and Her-2 in patients with Ⅲ + Ⅳ stages were significantly higher than those with Ⅰ + Ⅱ stages(respectively, P =0.014 and P =0.005). The positive rate in patients with T3+T4 stages were significantly higher than those with T1+T2 stages (P=0.033); and the positive rate in patients with positive lymph node was higher significantly than that with negative (P =0.033). The OS and PFS in over-expression patients were significantly longer than low-expression. Conclusion The overexpression of EGFR and Her-2 could be used to evaluate the degree of differentiation and prognosis of patients with gastric cancer.

Portal vein arterialization by right renal artery in portacaval shunt rats / 中华普通外科杂志

Objective To estabhsh a rat portacaval shunt model of portal vein arterialization (PVA)by using right renal artery,andtoinvestigateitsinfluence on portal velnpressure,liver funciton and hepatic pathology.Method Forty-three Sprague-Dawley rats were assigned to PVA group(33 rats)and control group(10 rats).Portal pressure,liver function and pathological changes were evaluated at day 2,2 weeks and 2 months after surgery,respectively.Results The experimental model was successfully established in 30 out of 33(91%)rats.At day 2,2 weeks and 2 months after the operation,the portal pressure increased significanfly(P<0.05)compared with the control group,but no significant differences were found in the senrum level of alanine amlnotransferase,total bilirubin,total bile acid and creatinine.There were no patholgiccal changes from day 2 to 2 weeks after surgery,only postoperative expanslon of the sinusoidal snace was found on 2 months after surgery.Conclusion The rat model of PVA by renal artery was successfully established with the hand-suture mierosurgical techniques.The portal vein pressure maintained at a high level after PVA,meanwhile,no negative effect was found on the liver function and hepatic histological structure changes.

Effect of fleabane injection on serum D-dimer, fibrinogen and hypersensitive C-reactive protein in patients with IgA nephropathy / 中国综合临床

Objective To observe the effect of fleabane injection on serum level of D-dimer, fibrinogen and hypersensitive C-reactive protein in patients with IgA nephropathy and to explore the mechanism of fleabane injection for treating IgA nephropathy. Methods 29 patients with IgA nephropathy were given fleabane injection together with routine treatment. Another group of 28 patients with IgA nephropathy were only treated with routine treatment as con-trol. Determinations of 24 hours urine protein output(24HPQ), serum level of D-dimer(D-D), Fibrinogen(Fib) and hypersensitive C-reactive protein (hsCRP) were carried out pre- and post-study. Results Significant decrease in 24HPQ , serum level of D-D, Fib and hsCRP were observed in both treatment group and control group(P <0. 01 ～0. 05), but more significant in treatment group as compared with control group ( P < 0. 05). Conclusion Flea-bane Injection plus routine treatment could significantly decrease 24HPQ in patients with IgA nephropathy, and this maybe contribute to regulation of D-D, Fib and hsCRP by fleabane injection.

Clinical Observation on Treatment of 43 Cases of Diabetic Vesical Pathological Changes by Needle-warming Moxibustion / 针灸推拿医学（英文版）

By the needle-warming moxibustion, 43 cases of diabetic vesical pathological changes,together with 43 cases in the control group, were treated for 30 days, to observe the times of urination,volume of urine and residual urine inside the bladder before and after treatment every day. It has been indicated in observation that needle-warming acupuncture therapy can decrease the time of urination every day, elevate the urine volume each time and decrease the residual urine in the bladder, with a significant difference (P＞0.05) in comparison with before the treatment.

INVESTIGATION ON ASSOCIATION OF SERUM BILIRUBIN CONCENTRATION WITH RISK OF CORONARY HEART DISEASE / 中国医师进修杂志

Objective　 To investigate the relationship between of serum bilirubin concentrations and risk of coronary heart drsease (CHD). Methods　 Serum bilirubin concentrations were determined in 12 cases with acute myocardial infarction(AMI)、37 cases with unstable angina pectoris (UAP) and 30 cases with UAP with arrhythmia. Results　 The serum total bilirubin(TB)、direct bilirubin(DB)、indirect bilirubin(IB) concentrations were lower in the three study groups of CHD than in the controls. There was significant difference between the patients with CHD and the controls. However, there was no significant difference among the three study groups of CHD. Conclusion　 Serum bilirubin concentrations was inversely and statistically significantly related to risk of CHD.

The Prevention and Therapeutic Effects of Hsp70-Antigen Peptide Complexes on B16 Tumor Pulmonary Metastasis in Mice / 中国肿瘤生物治疗杂志

Objective: To explore the preventive and therapeutic effects of Hsp70-antigen peptide complexes (HACs) on pulmonary metastasis of malanoma B16 cells in mice. Methods: Antigen peptide mixtures were prepared from massive tumors and metastatic foci respctively, and bound with Hsp70 in vitro. The HACs were used to mice to prevent the formation of micrometastatic foci after the injection of B16 cells through tail vein, or treat the existent micrometastatic foci in lung The number of metastatic foci was counted and the cytotoxicity of splenocytes to B16 cells was determined. Results: After immunization with HACs, the number of metastatic foci in mice decreased significantly (P

Effect of sevoflurance postconditioning on cardiac function and mitochondrial of isolated rat heart with acute myocardial ischemia-reperfusion injury / 中国药理学通报

0.05).After reperfusion,Sevo group and Post group resulted in a significant increase(P