Clinical study of the impacts of obesity and body weight change on the recurrence rate of colorectal adenoma / 重庆医学

Objective To observe the recurrence rate of colorectal adenoma and to explore its correlations to obesity and body weight changes.Methods A total of 1 236 cases of patients with colorectal adenoma admitted to our hospital from 2010 to 2012 were selected.Among them,913 cases of patients who had completed the 2-years follow-up were recruited in this study.According to body mass index (BMI),patients were divided into three gorups:normal weight group (BMI＜24 kg/m2),overweight group (BMI:24-＜28 kg/m2) and obesity group (BMI≥28 kg/m2).Colonoscopy was defined as the end-point performed after 2-years follow-up,and the body weights were remeasured.The correlations of recurrence rate of colorectal adenoma to patients' basal body mass and body weight change were analysed.Results A total of 361 patients (39.5％) suffered from recurrent colorectal adenoma.The recurrence rates of colorectal adenoma in the normal weight group,overweight group and obesity group were 34.5 ％,41.0％ and 41.9 ％,respectively;the recurrence rates in the overweight group and obesity group were higher than that in the normal weight group,there were statistically significant differences (P＜0.05).However,There was no significant difference in the recurrence rate of colorectal adenoma between patients with body weight changes of 2.5 kg or more and those with body weight changes less than 2.5 kg(P＞0.05).Conclusion The recurrence of colorectal adenoma is associated with obesity,but changes in body weight in the short term (two years) have no significant effect on the recurrence rate.

Clinical study of the impacts of obesity and body weight change on the recurrence rate of colorectal adenoma / 重庆医学

Objective To observe the recurrence rate of colorectal adenoma and to explore its correlations to obesity and body weight changes.Methods A total of 1 236 cases of patients with colorectal adenoma admitted to our hospital from 2010 to 2012 were selected.Among them,913 cases of patients who had completed the 2-years follow-up were recruited in this study.According to body mass index (BMI),patients were divided into three gorups:normal weight group (BMI＜24 kg/m2),overweight group (BMI:24-＜28 kg/m2) and obesity group (BMI≥28 kg/m2).Colonoscopy was defined as the end-point performed after 2-years follow-up,and the body weights were remeasured.The correlations of recurrence rate of colorectal adenoma to patients' basal body mass and body weight change were analysed.Results A total of 361 patients (39.5％) suffered from recurrent colorectal adenoma.The recurrence rates of colorectal adenoma in the normal weight group,overweight group and obesity group were 34.5 ％,41.0％ and 41.9 ％,respectively;the recurrence rates in the overweight group and obesity group were higher than that in the normal weight group,there were statistically significant differences (P＜0.05).However,There was no significant difference in the recurrence rate of colorectal adenoma between patients with body weight changes of 2.5 kg or more and those with body weight changes less than 2.5 kg(P＞0.05).Conclusion The recurrence of colorectal adenoma is associated with obesity,but changes in body weight in the short term (two years) have no significant effect on the recurrence rate.

DNA methylation and affective disorder / 中华行为医学与脑科学杂志

Objective Recent years,epigenetics research mainly focused on DNA methylation.A growing body of research indicated that DNA methylation may play vital role in etiology of affective disorder.With brief introduction of DNA methylation molecular mechanisms and relevance of DNA methylation to affective disorder,this review focuses on some supporting evidence which are recently emerged in major depressive disorder (MDD) and bipolar disorder related gene DNA methylation.Methods We retrievaled related articles from databases,such as PUBMED,Foreign medical information resources retrieval platform,CNKI,Wanfang database,ect,from 2013-06 to 2015-01.Index strategy TI:[depression OR bipolar disorder OR affective disorder] AND All field:DNA methylation.Reading and narrowing the scope of the articles,the experimental design was understanded,such as DNA from either blood cell or brain parenchyma of sample,methods of methylation detection,gene detected and methylation position,etc.The articles included 168 in English and 32 in Chinese.The whole and retained the articles were red about MDD and bipolar disorder related gene DNA methylation in recent five years.Results Eventually,the article includes 29 articles and two of in Chinese.Most of them are receptor gene epigenetics research associated with disease.Conclusion MDD and bipolar disorder are associated with the higher of DNA methylation levels in multiple gene promoter region,such as GR,BDNF,5-HTR,SLC6A4 and the lower of COMT.This article only from the perspective of affective disorder reviewed the relationship between DNA methylation and diseases.None of other diseases epigenetics research,there is to be perfected in future.

The research progress of depression with suicide and event related potential / 中华行为医学与脑科学杂志

Objective ERP research has always been the hot spot of the n~ve electrophysiology research.A gTowing body of research suggests that ERP can effectively reflect the cognitive function in patients with depression,evaluate the risk of suicide.This paper reviews the ERP related components with depression and related research.On the basis of the application of ERP in the depression research discusses limitations and prospects on the later development.Methods The articles were retrievaled from databases,such as P UBMED,Foreign medical information resources retrieval platform,CNKI,Wanfang database,ect,from 2013-06 to 2015-01.Index strategy TI:TI:[depression OR affective disorder] AND [suicide OR suicidal] All field:event related potential.Reading and narrowing the scope of the articles,we should understand the experimental design,such as Sample company have suicidal ideation and (or) behavior of patients with depression.Potential induced way,ERP related component detection method,etc.The articles included 43 in English and 15 in Chinese.Read the full text and retain nearly five years about depression and related literature.Results Eventually,the article includes 34 articles and 3 of in Chinese.Conclusion Associated with suicidal ideation and (or) behavior of the existing cognitive dysfunction in patients with depression,ERP related components such as:abnormal P300 and CNV,MMN.N400 have not found abnormal.This article only from the Angle of depression and related research are reviewed in this paper the characteristics of ERP,ERP of other mental illnesses among related research,and to be perfect in the future.

Effect of Berberine on AQP4 Expression in Focal Cerebral Ischemia Area in Diabetic Rats / 中国医科大学学报

Objective to investigate the effect of berberine on the expression of AQP4 and neuronal injury after focal cerebral ischemia in diabetic rats. Methods Male SD rats were randomly divided into four groups:control(sham surgery),middle cerebral artery occlusion and reperfusion (MCAO/R),MCAO/R treated with vehicle(DMSO),MCAO/R treated with berberine. the transient focal ischemia/reperfusion was induced by in-troducing silicone-coated monofilament nylon suture from the right external carotid artery into the origin of the middle cerebral artery,which was re-moved after 60 min. In group treated with berberine,the rats were injected with berberine before and after suffered from cerebral ischemia. Similarly, in group vehicle,the animals received DMSO vehicle at the same time. the score of neurological behavior was evaluated 24 h after reperfusion. Mean-while,the rats were sacrificed for Nissl staining. to estimate cerebral edema,the wet-dry ratio was measured. the expression of AQP4 in the border of the infarct region in different groups was observed by Western blot. Results Compared with the model group,berberine improved neurological deficits(P < 0.05). Berberine treatment inhibited the neuronal deformation shown by Nissl staining(P < 0.05). Berberine significantly decreased the wet-dry ratio and reduced the expression of AQP4(P < 0.05). Conclusion these results suggested that berberine could induce neuroprotection against ischemic injury by inhibiting the expression of AQP4 in diabetic rats.

Thought and method of reproductive toxicity research in traditional Chinese medicine / 药学学报

Reproductive toxicity research takes an important place in traditional Chinese medicine pre-clinical safety evaluation. Modern reproductive toxicity experiment includes drug-related miscarriage, fetal death, teratism, and adverse effects on fertility, genital system, embryonic development and fetus, which is different from contraindicated in pregnancy in traditional Chinese medicine theory. Now the three-phases reproductive toxicity study is the method mainly applied in traditional Chinese medicine reproductive toxicity evaluation. Besides that, alternative methods of whole embryos culture and embryonic stem cell test are also used in traditional Chinese medicine embryo toxicity evaluation. This article reviews research progress and pre-clinical evaluation on reproductive toxicity of traditional Chinese medicine.

Embryotoxicity of Senecionis Scandentis Hebra on in vitro cultured mouse embryos / 药学学报

The purpose of this study is to evaluate the embryotoxicity of alkaloids in Senecionis Scandentis Hebra on in vitro cultured mouse embryos. Mouse whole embryo culture (WEC) was applied in this study. Post-implantation (8.5 d) mouse embryos were isolated from their mothers, and cultured in medium of immediately centrifuged serum (ICS) with different concentrations of seneciphylline (target concentrations were 100, 50, 25 and 12.5 μg x mL(-1)) or senkirkine (target concentrations were 50, 25 and 12.5 μg x mL(-1)) for 48 h. After culturing completed, the development and organic morphodifferentiation of the cultured embryos were evaluated microscopically. Treatment with seneciphylline and senkirkine had adverse effects on the development and organic morphodifferentiation of embryos. The effect also had clear dose-response. Alkaloidals in Senecionis Scandentis Hebra had embryotoxicity on cultured embryos, which indicated that pregnant people exposed to Senecionis Scandentis Hebra may get potential risk on fetus.

Construction of Marek's disease virus serotype 814 strain as an infectious bacterial artificial chromosome / 生物工程学报

The aim of this study was to construct the complete genome of Marek's disease virus serotype 814 strain as an infectious bacterial artificial chromosome (BAC). Using self-designed selection marker Eco-gpt (1.3 kb) and BAC vector pBeloBAC11 (7.5 kb), we constructed the transfer plasmid pUAB-gpt-BAC11. The plasmid pUAB-gpt-BAC11 and MDV total-DNA were cotransfected into secondary CEFs; we put the virus-containing cells in selection medium for eight rounds and obtained purified recombinant viruses. Recombinant viral genomes were extracted and electroporated into E. coli, BAC clones were identified by restriction enzyme digestion and PCR analysis. Finally, we obtained 38 BAC clones, DNA from various MDV-1 BACs was transfected into CEFs, and recombinant virus was reconstituted by transfection of MDV-BAC2 DNA. We successfully cloned the complete genome of MDV-1814 strain as an infectious bacterial artificial chromosome. With these cloned genomes, a revolutionary MDV-DNA engineering platform utilizing RED/ET recombination system was constructed successfully, which can help the understanding of MDV gene functions and promote the using of MDV as a vector for expressing foreign genes. In addition, it opens the possibility to generate novel MDV-1 vaccines based on the BACs.