Seroepidemiological Survey of Hydatid cyst by ELISA in Kordestan Province

This study was conducted to evaluate the frequency of human hydatidosis in Kurdestan province by ELISA technique. In this study the sera of 1979 individuals were collected from different area [cities and villages] of Kordestan province. The serum dilution of 1/400 was selected for ELISA test. The results indicated that 1.12% of the individuals from Kordestan province showed positive sera. The results also showed that in Kordestan 0.9% and 1.42% of the people who live in the cities and villages had positive sera respectively. In this study 1.65% of female and 0.45% of male were positive. From the obtained result we found maximum number of infected people were in the range of 30-40 years [1.59%]. According to the results obtained from this study the highest percent of infection was found in the city of Ivandarre, the reason for this difference [1.69%] is due to the fact that most of the people who are involved in animal husbendary in the province live in this city

[Study on variation of the sera folic acid, vitamin B12 and iron level in the 6-12 years old patients infected with Giardia lamblia in south Tehran]

Protozoa Giardia lambelia is caused to diarrhea in human and other mammals in worldwide. Giardia colonizes in duodenum and earliest jejunum. It can cover the intestinal surface and causes the stateorrhea, malabsorbtion syndrome and absorb disorder of vitamins A, E and D. Investigation the effect of Giardia on the vitamin B12, folic acid and iron of patients were the purpose of this study. A total of 30 children with giardiasis, aged between 6-12 years-old, selected from 3000 patients, who were admitted to the Emam Khomeiny hospital and diagnosed by stool examinations [direct and formalin-ether methods]. Blood of patients and control were collected. Amount of vitamin B12 and folic acid were evaluated with radioimmunoassay and iron was evaluated with Ferene method. According to the data, folic acid in the patient had no significant difference against control but vitamin B12 and iron in the patient with giardiosis was less than control and the differences were significant [P=0.01, P=0.04 respectively]. According to the results diagnosis of giardiosis in the early stage may prohibit intestinal damage and clinical symptoms due to vitamins and mineral elements shortage in the children