RESUMO
Background: Spermatogonial stem cells are the foundation of spermatogenesis and male fertility. So, their maintenance and culture are very important
Objective: In this study, we assessed protective effects of the Calligonum on in vitro viability and apoptotic and antiapoptotic genes expression of spermatogonial stem cells
Materials and Methods: After 24 hr of culture, the spermatogonial stem cells were treated with 30 MuM dose of H[2]O[2] and then 10 Mug/ml the Calligonum extract was added for 3 wks. Viability was assessed by Trypan blue, apoptosis using PI-Annexin and finally Bax, Bcl-2 and P53 genes expression by Real-Time Polymerase chain reaction
Results: After 3 wk of treatment, viability in the Calligonum extract+H[2]O[2] group was significantly higher than H2O2 group alone [p=0.001]. In the Calligonum extract+H2O2 group, apoptosis, as well as expression of apoptotic genes [Bax and P53], was significantly lower than the group treated with H[2]O[2] alone
Conclusion: The results of this study showed that 30 MuM H[2]O[2] increased apoptosis but decreased viability in spermatogonial stem cells. Calligonum has antioxidant properties that can reduce apoptosis, Bax and P53 expression and increase the viability and Bcl-2 expression