RESUMO
Objective:To express Gag gp120 fusion protein in the recombinant fowlpox virus.Methods:The Gag gp120 gene of HIV 1 was inserted downstream of the combined promoter in pUTAL,an expressing plasmid was constructed.By transfecting the plasmid into CEF cells pre infected with FPV 282E 4 strain,a recombinant fowlpox virus vUTALG was obtained by selecting in the presence of BUdR and subsequent blue plaque screening.Results:Western blot showed that the recombinant virus expressed the Gag gp120 fusion protein in the infected CEF cells.The virus like particles formed by Gag protein were observed under electron microscope.Mice were immunized with the recombinant virus.The results showed the recombinant virus could induce HIV 1 specific antibody response.Conclusion:The recombinant fowlpox virus could express the Gag gp120 fusion protein.
RESUMO
Chemical terror is one of the main forms of terrorism. The proliferation of chemical weapon and abuse of chemical poisons make chemical terrorism flourish and diversified in occurrence. The features of chemical terrorism are unexpectedness, surreptitiousness, diversity in their vehicles and media, and it could be widespread to involve a broad area to create a serious tumult. Medical protection is the pivotal step to control the influence of chemical terror. Anti-chemical terror medicine will provide new important subjects for researches in chemical-defense medicine, and studies in chemical-defense medicine will lay the foundation of anti-chemical-terror medicine. In conclusion, it is imperative that the principles, main subjects, and strategies of research on anti-chemical-terror medicine should be established as early as possible.